Coronavirus infection of the bovine respiratory tract

Two viruses, morphologically resembling coronaviruses and antigenically indistinguishable from bovine enteric coronavirus, were isolated in bovine tracheal organ cultures from the lungs and trachea of young calves with respiratory disease. Intranasal and intratracheal inoculation of these viruses into neonatal calves resulted in a predominantly upper respiratory tract infection, which was associated with the development of mild respiratory symptoms.     

Conversion of wastes into bioelectricity and chemicals by using microbial electrochemical technologies

Waste biomass is a cheap and relatively abundant source of electrons for microbes capable of producing electrical current outside the cell. Rapidly developing microbial electrochemical technologies, such as microbial fuel cells, are part of a diverse platform of future sustainable energy and chemical production technologies. We review the key advances that will enable the use of exoelectrogenic microorganisms to generate biofuels, hydrogen gas, methane, and other valuable inorganic and organic chemicals. Moreover, we examine the key challenges for implementing these systems and compare them to similar renewable energy technologies. Although commercial development is already underway in several different applications, ranging from wastewater treatment to industrial chemical production, further research is needed regarding efficiency, scalability, system lifetimes, and reliability.     

A glycyl radical site in the crystal structure of a class III ribonucleotide reductase

Ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides. Three classes have been identified, all using free-radical chemistry but based on different cofactors. Classes I and II have been shown to be evolutionarily related, whereas the origin of anaerobic class III has remained elusive. The structure of a class III enzyme suggests a common origin for the three classes but shows differences in the active site that can be understood on the basis of the radical-initiation system and source of reductive electrons, as well as a unique protein glycyl radical site. A possible evolutionary relationship between early deoxyribonucleotide metabolism and primary anaerobic metabolism is suggested.     

细化设备管理 提高使用效益

随着高等教育事业的发展,国家对高校设备经费投入也逐年增加,但目前的投入远不能满足高等教育事业快速发展的需求。同时,普遍存在的一个情况是,各个高校的设备使用率较低,资源闲置。在有限的资金投入下,如何提高现有设备使用效益,成为最有效的解决这一瓶颈的关键。文章根据云南大学教学科研设备使用情况,结合信息学院目前实验室现有仪器设备及教学任务,探讨了一些高校仪器设备管理的经验和方法。尤其探讨了学校根据实际情况,自主研发了资产管理系统,进一步规范了资产管理工作,提高了设备使用效益,特别是针对大型仪器设备,建立共享平台,实行预约制度,充分体现了大型设备的资源优势。     

Space use and inferred spawning activity of three exploited gamefish species on a large artificial reef

Intense fishing pressure has led to changes in species composition, demography and the over‐exploitation of many coastal fishes. To restore populations, artificial reefs (ARs) are used with the assumption that new habitat will increase fish productivity. Kelp bass (Paralabrax clathratus), barred sand bass (P. nebulifer) and California sheephead (Semicossyphus pulcher) are three important recreational gamefish species in southern California. Home ranging behaviour, space use and spawning‐related activity of these three species were quantified on a large AR. Kelp bass used the largest amount of area, but space use was similar among species. While no spawning behaviour could be determined for sheephead, kelp bass exhibited two different activity patterns during spawning months and barred sand bass migrated (indicative of spawning). Results of this study indicate that this large AR is providing suitable habitat for these species, and spawning‐related activity could be a sign that this AR is meeting essential life history demands.     

电控发动机尾气检测与故障诊断的研究

为寻求根据汽车尾气参数来诊断汽车故障的方法，利用径向基函数（RBF）神经网络，对丰田雷克萨斯（LEXUS）400电控发动机进行故障诊断研究。研究结果表明，径向基函数神经网络对发动机故障的诊断是迅速、准确的。     

锦江鱼类资源现状与物种多样性分析

2013年8月—2016年8月对沅江水系支流锦江的鱼类资源现状进行了调查。通过向渔民购买、现场捕捞等方式,共调查野生鱼类43种,隶属于5目11科39属,其中鲤科鱼类25种,占总种类数的58.1%。调查到新记录种5种,分别为大鳞副泥鳅(Paramisgurnus dabryanus)、吻(Rhinogobio typus)、洞庭华鲮(Sinilabeo tungting)、云南盘(Discogobio yunnanensis)和大眼鳜(Siniperca kneri)。干、支流均以底层鱼类为主,种类数分别占各自总数的55.8%和71.4%。对比历史资料,G-F多样性指数呈下降趋势。对干流渔获物种类的周年统计发现,出现频率在50%以上的鱼类有8种,其中泥鳅(Misgurnus anguillicaudatus)、鲤(Cyprinus carpio)、鲫(Carassius auratus)和鲇(Silurus asotus)的出现频率均达到97.3%。     

不同环境因子对草履虫种群生长的影响

为了研究不同环境因子对草履虫种群生长的影响,培养出优质的草履虫,分别在pH梯度为:6.0、6.5、7.0、7.5、8.0,光照梯度为:0lx、500lx、1 000lx、1 500lx条件下培养草履虫,结果表明,小白菜培养液培养草履虫的最适pH值为7.0,并且相比于弱酸性环境,草履虫更适宜在弱碱性环境中生长。在光照为0lx条件下,草履虫数量在第4天时达到最大值4 073ind/100mL,在光照为500~1 500lx条件下,随着光照度的增加,草履虫的种群数量也随之增加。     

基于模型的四足机器人步态转换控制研究

为了解决基于模型的控制方法在四足机器人步态转换过程中稳定控制问题,本文在仿生学和机构学基础上设计了一款四足机器人样机平台,并推导了机器人单腿运动学模型。在足端可达工作空间内规划了机器人抬腿高度和迈步步长,利用理想的复合摆线轨迹,通过合理控制步态周期,提出了一种过渡段变周期控制方法,实现了步态转换前后定速度控制和变步长控制,保证了步态转换前后速度不变或可变。为了验证所提算法的正确性和稳定性,分别开展了单腿足端轨迹实验和整机步态转换实验。在完成整机运动控制的基础上,对比了基于模型的控制方式和基于中枢模式发生器的控制方式在四足机器人步态转换过程的应用。仿真和实验结果表明,在基于模型的控制算法下,四足机器人可以实现步态的平滑转换,且速度能伴随步长和周期的变化实现调节,满足了不同速度下的行走要求,为四足机器的运动控制提供了参考。     

重金属污染土壤原位钝化修复研究进展

\t\t\t\t\t目的\t\t\t\t\t探究根施脯氨酸对铬胁迫下小麦幼苗生长的影响。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t以小麦品种轮选988为材料,采用溶液培养法,研究30 mg/L脯氨酸对160 mg/L Cr³⁺胁迫下的小麦根系的生理影响。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\tCr³⁺胁迫严重抑制小麦生长。与单独Cr³⁺胁迫相比,根施脯氨酸后,小麦幼苗根长、株高、茎叶鲜重、茎叶干重、根鲜重和根干重分别上升了49.4%、17.9%、21.7%、35.4%、52.0%和38.7%;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)分别升高了8.51%、4.50%、8.19%和9.74%;引起超氧阴离子(\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPuepose\t\t\t\t\tThe effect of proline on chrome (Cr³⁺) toxicity in wheat (Triticum aestivum L.) was studied.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tMethod\t\t\t\t\tWheat seedlings exposed to 160 mg/L Cr³⁺ were treated with 30 mg/L proline.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tResults\t\t\t\t\tCr³⁺ exposure depressed wheat growth. Compared with the single Cr³⁺ stress, after proline application, the root length, plant height, fresh weight of stem leaf, dry weight of stem leaf, root fresh weight and root dry weight of the seedlings increased significantly by 49.4%, 17.9%, 21.7%, 52.0% and 38.7%, respectively. And the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate perxoidase (APX), increased by 8.51%, 4.50%, 8.19% and 9.74%, respectively; the contents of superoxide anion (O₂⁻·), hydrogen peroxide (H₂O₂), and malondialdehyde (MDA), rise significantly by 60.32%, 24.56% and 34.20%, respectively. Ultimately, the root activity increased by 1.67 times.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tConclusion\t\t\t\t\tTherefore, exogenous proline could significantly improve the antioxidant capacity of wheat roots, and then it could enhance the resistance ability of wheat and alleviate the toxic effect of chrome on wheat seedlings.\t\t\t\t