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51.
Mapping human brain monoamine oxidase A and B with 11C-labeled suicide inactivators and PET 总被引:1,自引:0,他引:1
J S Fowler R R MacGregor A P Wolf C D Arnett S L Dewey D Schlyer D Christman J Logan M Smith H Sachs 《Science (New York, N.Y.)》1987,235(4787):481-485
The regional distributions of monoamine oxidase (MAO) types A and B have been identified in human brain in vivo with intravenously injected 11C-labeled suicide enzyme inactivators, clorgyline and L-deprenyl, and positron emission tomography. The rapid brain uptake and retention of radioactivity for both 11C tracers indicated irreversible trapping. The anatomical distribution of 11C paralleled the distribution of MAO A and MAO B in human brain in autopsy material. The corpus striatum, thalamus, and brainstem contained high MAO activity. The magnitudes of uptake of both [11C]clorgyline and L-[11C]deprenyl were markedly reduced in one subject treated with the antidepressant MAO inhibitor phenelzine. A comparison of the brain uptake and retention of the 11C-labeled inactive (D-) and active (L-) enantiomers of deprenyl showed rapid clearance of the inactive enantiomer and retention of the active enantiomer within MAO B-rich brain structures, in agreement with the known stereoselectivity of MAO B for L-deprenyl. Prior treatment with unlabeled L-deprenyl prevented retention of L-[11C]deprenyl. Thus, suicide enzyme inactivators labeled with positron emitters can be used to quantitate the distribution and kinetic characteristics of MAO in human brain structures. 相似文献
52.
53.
Rock avalanches blocked streams in the Olympic Mountains southwest of Puget Sound during the past few thousand years. Limiting radiocarbon ages indicated that three or four of six avalanches occurred from 1000 to 1300 years ago or shortly thereafter. Most of the dates were from the outer preserved rings of trees drowned behind avalanche dams. These three or four avalanches may be coeval not only with one another but also with abrupt tectonic deformation in western Washington. No rock avalanches in the Olympic Mountains are known to have resulted from storms or earthquakes during the past century. The avalanches strengthen the case that a large prehistoric earthquake occurred in the Puget Sound region. 相似文献
54.
Ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides. Three classes have been identified, all using free-radical chemistry but based on different cofactors. Classes I and II have been shown to be evolutionarily related, whereas the origin of anaerobic class III has remained elusive. The structure of a class III enzyme suggests a common origin for the three classes but shows differences in the active site that can be understood on the basis of the radical-initiation system and source of reductive electrons, as well as a unique protein glycyl radical site. A possible evolutionary relationship between early deoxyribonucleotide metabolism and primary anaerobic metabolism is suggested. 相似文献
55.
A. G. Bobbal D. C. L. Lam D. S. Jeffries D. Bottomley J. Y. Charette P. J. Dillon L. Logan 《Water, air, and soil pollution》1986,31(1-2):155-163
Application of a hydrological model to three Canadian watersheds forms part of a verification package to test its accuracy and portability. Agreement of model results with observed data on hydrograph, groundwater flow and snowpack is reasonably good. The model results are consistent with the observed pH and with many of the episodic events that have occurred in these acidified watersheds. Contrasts have also been made on the different calibrated coefficients at several locations in these watersheds. An attempt to relate them to the geology and soil characteristics at the site has led to realistic estimation of the soil contact times. 相似文献
56.
Effects of 12 years of 4 tillage systems, were studied on soil bulk density, infiltration rate, penetration resistance, structural stability of aggregates, root length density, moisture release characteristics and grain yield. The objective of this study was to assess long-term effects of tillage methods and guided traffic on soil properties and crop response on a heavy-textured poorly drained soil. Tillage systems included: (A) continuous no-till for 12 years; (B) no-till for 10 years followed by plow-till for 2 years; (C) plow-till for 10 years followed by no-till for 2 years; (D) continuous plow-till for 12 years. Wheel tracks had significant effects on soil physical properties. Soil in the traffic zone (TZ) in no-till treatment had higher bulk density and penetration resistance (PR) for the upper 0–30-cm layer than plow-till treatment. The PR for the surface layer in TZ was 25–46% more than in the row zone (RZ). Mean soil bulk density in the TZ of no-till plots was about 12% more than in the RZ (1.53 vs. 1.36 g cm−3). Changeover from no-till to plow-till decreased PR in RZ and TZ by 50–60% while that from plow-till to no-till increased PR by 10–20%. Similar effects were observed in percent aggregation and the mean weight diameter. In no-till treatments both initial and equilibrium infiltration rates were significantly lower in TZ than RZ (27.5 vs. 6.8 cm h−1 initial and 10.0 vs. 1.5 cm h−1 final). There were more macropores (> 2 mm) for the TZ in no-till compared with the plow-till treatments. Fine or micropores were comparatively more numerous in the sub-soil of no-till than plow-till treatments. The median aggregate size (D50) was 6.2, 4.2, 4.8 and 3.5 mm for Treatments A, B, C and D, respectively. Root length density of corn in plow-till plots was significantly more than that in no-till plots for the top 0–20-cm layer. Corn and soybean yields were greater in plow-till than no-till treatments. Grain yields in Treatment D were greater than those in Treatment A by 10% for corn and by 6% for soybean. 相似文献
57.
纳他霉素处理对火龙果贮藏品质的影响 总被引:1,自引:0,他引:1
研究不同浓度的纳他霉素对火龙果采后贮藏保鲜效果的影响。以火龙果为试验材料,研究不同浓度(0、400、800、1200 mg/L)纳他霉素对火龙果贮藏期生理、营养指标及果实表面微生物菌落情况的影响。结果表明:与对照相比,不同浓度的纳他霉素处理均可显著降低火龙果呼吸强度,抑制腐烂指数、鳞片黄化率、质量损失率、丙二醛含量的增加,延缓鳞片叶绿素、可溶性固形物、可滴定酸、维生素C含量的降低,提高果实SOD、POD、CAT等抗氧化相关酶活性,在贮藏末期时仍能保持较低的细菌菌落总数、酵母菌和霉菌总数。综合认为,纳他霉素处理可以明显延缓火龙果果实衰老,有效保持果实的贮藏品质,其中以800 mg/L纳他霉素处理效果最佳。 相似文献
58.
冬枣溃疡病病原初步鉴定 总被引:3,自引:0,他引:3
2003~2004年在山东省滨州市沾化县、无棣县等冬枣产区调查发现,冬枣生长前期在枣吊和叶片上发生了一种细菌性新病害。通过对典型病害样本进行病原菌分离、纯化获得20个菌株。从中选出4个菌株进行致病性测定,同时对其细菌形态、培养性状、染色反应、生理生化测定及血清学反应等性状进行了较为系统研究。结果表明,该地区冬枣溃疡病是由油菜黄单胞Xanthom onas campestrisPamm e(Dowson,1939)侵染引起的。 相似文献
59.
D P Mackie D J Meneely D A Pollock E F Logan 《Veterinary immunology and immunopathology》1986,11(2):193-198
The role of the IgA antibody to Streptococcus agalactiae found in the whey of milks 12 hours after the first intramammary infection of six Friesian first lactation heifers was assessed using an in vitro bactericidal assay. The mean percentage kill of the streptococci by neutrophils in the presence of these wheys was 36.2% while the equivalent figure for the non-infected quarter whey was 0%. When the IgA antibody was absorbed from the infected quarter wheys using class specific IgA antiserum cross linked with glutaraldehyde the percentage kill of the test system fell to 0%. Elution of the absorbed antibody partially restored the activity to a mean percentage kill of 18.2%. The results indicated that the IgA antibody found in infected quarter whey during the acute stages of intramammary infection with Streptococcus agalactiae was responsible for the opsonic activity which pertained at that time. 相似文献
60.
以与东北马鹿物种最近的牛(Bos taurus cattle,序列号为AB076403)的肌肉生长抑制素(myostatin,MSTN)基因序列为模版,根据该基因保守序列进行引物设计,扩增产物连接入pMD18-T载体,克隆出3个外显子片段.根据5′-GU-AG-3′规则和同源基因比对拼接出带有部分5′、3′末端的cDNA,并采用生物信息学技术对编码氨基酸序列进行蛋白质同源性比较和二级、三级结构等分析.结果表明:所获序列全长为1 128 bp,编码375个氨基酸;具有较明显的螺旋、片层和无规卷曲等二级结构;有1个较明显的跨膜区和1个疏水区,并发现明显的信号肽和糖基化位点.三级结构同源建模预测结果显示与Human Activin A的相似性为42%.结果说明东北马鹿和其他哺乳类生物的肌肉生长抑制素基因之间关系密切,并同属于TGF-β家族. 相似文献