药用红花生长发育规律的研究

根据药用红花根、叶、茎、分枝、花及生长中心的变化特点，将药用红花个体发育过程划分为莲座期、伸长期、分枝期、开花期及种子成熟期５个生育时期，红花一生有５２叶左右，以伸长期出叶速度最快，平均每２．０天出一叶，这一时期形成１７叶左右。叶面积在生育前期较小，至分枝期达最高值（４月中旬，３０７４．５ｃｍ＾２／ｐｉａｎｔ，叶面积指数５．５），且保持较高叶面积一定时间，以利于分枝和花的发育。红花在分枝期净同化率     

基于时序NDVI图谱库提高土地覆盖分类精度的方法

为提高MODIS土地覆盖产品的分类精度,该文以河南省为试验区,首先将MODIS土地覆盖产品(MCD12Q1)分为高精度区域和低精度区域,然后通过构建时序NDVI图谱库并利用图谱曲线相似性测定方法,改进MCD12Q1低精度区域的分类精度。结果表明:1)时序NDVI是土地覆盖的重要分类特征,二者之间具有较强的关联性。2)利用时序NDVI图谱库能够明显提高MODIS土地覆盖产品的分类精度,改进后的MCD12Q1的总体分类精度分别由72.76%(比较评价)、64.52%(样本评价)提高到83.05%和81.72%。3)不同土地覆盖类别精度提高的程度不同,林地、草地、耕地、人工地表以及水体的生产者精度分别提高35.36%、29.51%、2.98%、6.96%和6.11%。4)对于判定时序NDVI曲线相似度的2种具体方法而言,最小距离法(minimum distance,MD)总体上优于光谱角度匹配法(spectral angle mapper,SAM)。综上,保留现有土地覆盖产品中分类精度较高的部分,基于时序NDVI图谱库改进分类精度较低的部分,是提高现有土地覆盖产品分类精度的有效方法。     

Isomerization of trans-astaxanthin induced by copper(II) ion in ethanol

Carotenoids are unstable and susceptible to disruption by environmental factors such as heat, light, and solvents. However, there is little information on the effect of metal ions on stability of carotenoids, especially those essential elements in human nutrition. Astaxanthin is one of the few carotenoids containing four oxygen donors. Usually, these oxygen donors can coordinate with heavy metal ions such as Cu(II) and Fe(III). In the present study, the interaction of trans-astaxanthin with Cu(II) was examined. It was found that Cu(II) markedly induces the conversion of trans-astaxanthin to its cis forms, which mainly consist of 9-cis-astaxanthin and 13-cis-astaxanthin as suggested by UV-visible spectra and HPLC measurements. Increasing either incubation time of Cu(II) and trans-astaxanthin in ethanol or the Cu(II)/astaxanthin ratio results in an increased percentage of cis isomers derived from trans-astaxanthin. All these results provide important information on the effects of dietary factors on the bioavailability and bioactivity of trans-astaxanthin.     

Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo

Cancer chemoprevention is employed to block or reverse the progression of malignancies. To date, several thousands of agents have been found to possess chemopreventative activity, one of which is capsaicin, a component of chili peppers that exhibits antigrowth activity against various cancer cell lines. However, the role of capsaicin in tumorigenesis remains controversial because both cancer prevention and promotion have been proposed. Here, we made the unexpected discovery that treatment with low concentrations of capsaicin up-regulates tNOX (tumor-associated NADH oxidase) expression in HCT116 human colon carcinoma cells in association with enhanced cell proliferation and migration, as evidenced by down-regulation of epithelial markers and up-regulation of mesenchymal markers. Importantly, tNOX-knockdown in HCT116 cells by RNA interference reversed capsaicin-induced cell proliferation and migration in vitro and decreased tumor growth in vivo. Collectively, these findings provide a basis for explaining the tumor-promoting effect of capsaicin and might imply that caution should be taken when using capsaicin as a chemopreventive agent.     

有机肥对不同母质菜田土壤磷解吸动力学模拟

通过室内恒温培养试验研究了华南3种典型母质发育的菜田土壤(玄武岩母质发育、花岗岩母质发育、河流冲积物母质发育)在经过两种有机肥(鸡粪堆肥和商品有机肥)预培养30 d和60 d后其土壤有效磷含量及磷解吸动力学过程的影响。结果表明,添加鸡粪堆肥和商品有机肥后,3种母质发育菜田土壤其有效磷含量均显著增加,且添加鸡粪堆肥处理的土壤其有效磷含量的增加量大于添加商品有机肥处理;3种母质发育土壤在添加两种有机肥处理后其磷解吸动力曲线均是先快速解吸然后缓慢达到平衡的过程;磷解吸量不仅与各处理的土壤初始有效磷含量密切有关,而且与土壤成土母质类型、施入有机肥种类相关;3种母质发育的菜田土壤在经过两种有机肥处理后其磷解吸动力曲线用5种动力学方程进行拟合,发现Elovich方程、抛物线方程和幂函数方程能够取得较好的拟合效果。     

RAPID DIAGNOSIS OF NUTRIENT ELEMENTS IN FINGERED CITRON LEAF USING NEAR INFRARED REFLECTANCE SPECTROSCOPY

In order to provide references for leaf nutrition diagnosis of fingered citron, the technique of near infrared reflectance spectroscopy (NIRS) was introduced to analyze nitrogen (N), phosphorus (P), potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) in the dry-leaf samples of fingered citron. The best calibration model for N was developed with high RSQ_CAL (0.90), SD/SECV (2.73) and low SEC (1.06 mg g^?1), good calibration models were obtained for P, K, Fe and Mn, and no significant correlations were found between the spectra and the individual amounts of Zn and Cu. When tested using a validation set (n = 38), N was well predicted with low values of SEP (1.21 mg g^?1) and high RPD (2.5). The values of SEP and RPD were also acceptable for the external validation of P, Fe and Mn. Near-infrared spectroscopy analysis technique shows potential of diagnosing minerals in fingered citron, particularly for N, P, Fe and Mn.     

Single-run electrochemical determination of melamine in dairy products and pet foods

A simple electrochemical approach, which does not require any expensive and complex instruments, is established for the selective and quantitative recognition of melamine in diary products and pet foods. During a preconcentration step (at 1.8 V versus Ag/AgCl), the formation of a polymer film from melamine on a preanodized screen-printed carbon electrode was identified by SEM and XPS. The as-formed polymer was found to be electroactive with a reversible redox peak, and hence square-wave voltammetry was applied to further increase the detection sensitivity to meet the detection limit for application in real sample analysis. Simply with a medium exchange procedure, melamine was selectively detected with a detection limit (S/N=3) of 0.8 μM (i.e., 98.3 ppb) by square-wave voltammetry. Lower than 1 ppm of melamine in real samples can be easily detected with good recoveries of 98.7-100.9% by the proposed approach. The recovery tests established for external calibration and standard addition techniques verified that the analysis can be done in a single-run measurement.     

Studies of Soils, Soil Water and Stream Water at a Small Catchment near Guiyang, China

Acid deposition is considered to be a major environmental problem in China, but information about effects on soils and waters is scarce. To contribute to increased knowledge about the problem a small catchment (about 7 ha) in the outskirts of Guiyang, the provincial capital of Guizhou in south-western China, was instrumented for collection of precipitation, throughfall, soil water and stream water. In addition soil samples have been collected and analyzed for key properties. Median pH in the precipitation is 4.40 (quartiles: 4.19 and 4.77) and the median sulfate concentration 228 µeq/L (quartiles: 147 and 334 µeq/L). The dry deposition of both SO₂ and alkaline dust is considerable. The sum of wet deposition of sulfate and dry deposition of SO₂ has been estimated to about 8.5 gSm^-2yr^-1. The total S-deposition may be somewhat higher due to dry deposition of sulfate and occult deposition. In soil water, SO₄ ^2- is the major anion, generally ranging from 300 to 2500 µeq/L in the different plots. Calcium is an important cation, but there is also a considerable contribution of aluminum from the soil. In some of the plots the concentrations of inorganic monomeric aluminum (Ali) are typically between 200 and 400 µm. Potential harmful levels of aluminum and/or high Ali/(Ca²⁺ + Mg²⁺) molar ratios occur in the catchment, but damages to vegetation have not yet been reported. In most cases exchangeable aluminum accounts for between 75 and 95% of the total effective cation exchange capacity (CEC_E) in the mineral soils. The aluminum chemistry cannot easily be explained by conventional models as the Gaines-Thomas ion-exchange equation or equilibrium with an Al(OH)₃ mineral phase. The stream water is generally less acidic and has considerably lower concentrations of aluminum than the soil water, even though quite acid events have been observed (pH < 4.4). The median pH values are 4.9 and 5.0 in the two first order streams and 6.3 in the dam at the lower boarder of the catchment.     

纸层析分离转氨作用产物方法的改进

本文介绍作者研制的一种用于纸层析分离转氨作用产物──氨基酸的展层剂,它含有乙醇、水和尿素（80：20：0．5）,与传统的展层剂苯酚和水相比,它具有价格便宜（节省经费217％）、安全无毒、操作方便等优点,是一种理想的展层剂。     

Dammarane-type glycosides from steamed notoginseng

Notoginseng, the root of Panax notoginseng (Burk.) F. H. Chen (Araliaceae), is a Chinese traditional medicine, which is used in both raw and processed forms due to their different pharmacological activities. Detailed chemical investigation on steamed notoginseng led to the isolation of 27 dammarane-type triterpenoids (1-27), including 4 new glycosides, namely, notoginsenosides ST-1-ST-3 and ST-5 (1-4), in addition to 3 other known compounds. Of the new compounds, 1-3 possess new aglycones. Their structures were elucidated on the basis of detailed analyses of their 1D and 2D NMR spectra and chemical reactions. The known compounds, koryoginsenoside-R1 (19), yesanchinoside D (20), 6'-O-acetylginsenoside Rg3 (24), and 3beta,6alpha,12beta-trihydroxydammar-20(21),24-diene ( 25), were isolated from notoginseng for the first time.