强麦期价呈现区间波动特征——2009年下举年强麦走势展望

1 行情回顾篇 纵览强麦上半年走势,其特征可以概括为"暴涨阴跌",主导行情的因素为:旱情炒作、强麦供需状况以及国家政策.     

产房内仔猪腹泻的原因及防治措施

仔猪腹泻是目前规模化养猪生产中最严重的仔猪疾病之一.产房内仔猪发生腹泻后,轻者可使患病仔猪体质下降和免疫功能减退从而导致其对其他疾病的抵抗力减弱,严重者可引起仔猪死亡,给养猪生产造成严重经济损失.     

液态奶中三聚氰胺检测方法（GC—MS）的比较

实验根据国标GB/T22388—2008“原料乳与乳制品中三聚氰胺GC—MS检测方法”与农业部饲料效价与安全检测中心的“牛奶中三聚氰胺的GC—MS”检测方法，对三聚氰胺添加量分别为0．05069mg／kg、0．40552mg／kg的牛奶样品进行了测定．对比了两种方法在相同条件下的检测限、回收率、稳定性、线性范围、基质干扰情况。实验结果表明：农业部饲料效价与安全检测中心的检测方法，在检测限、回收率、重复性、线性范围、方法灵敏度上较国标好。同时还发现．当液态奶中的三聚氰胺含量接近检测限时．两种方法的的检测结果受奶样基质的干扰较为明显。     

茄子早熟育种研究Ⅱ选择指数与早熟性选择

研究结果表明,由性状果实最大周长,果实体积,门茄显蕾期,单株产量等性状构成的选择指数较好;进一步进行遗传相关分析结果认为,应用选择指数时应注意果实体积这个性状,单株产量是一个重要的辅助性状,门茄显蕾期是一个限制性因子。应用选择指数对品种评价结果认为,早熟性较好的品种有:天津早茄、西安绿茄、二民、七叶茄、白线茄和汉中荷包。     

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)     

高美施对平菇母种生长发育的影响

本试验是在ＰＤＡ培养丛中，添加不同浓度的高美施微肥，通过两个供试菌株的对比试验，选出了适宜各菌抹的最佳高美施生长浓度，结果，不同苗株在不同浓反的高美施培养基中的在现不一致。因此．在制种工作中，要根据平菇不同品种的特征特性来确定其最佳生长的营养条件。高美施在平菇母种制种技术上的应用在国内属首次报道。     

云南省世界银行贷款“国家造林项目（NAP）”科技推广

本文报告了云南世行贷款“国家造林项目（ＮＡＰ）”的科技推广后所取得的成效。为了增加项目造林的科技含量，项目一启动就重视科技成果的推广，前后推广了省内外重要科技成果６项，对主要造林树种建立了试验林、示范林、环境保护监测样地，以及配套的科学管理等措施。通过这些举措，使项目造林取得显著成效，完成造林任务６４２７５．８１ｈｍ２（９６．４万亩），为协议任务面积的１４６．１％，造林成活率达９５％以上，保存率达９０％以上。由于生产与科技紧密结合，从而大大提高了科学造林水平，使“ＮＡＰ”项目在省内工程造林中起到了示范作用。     

In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.     

东北鼢鼠生活习性初探

本文通过对东北鼢鼠生活习性定位监测,初步掌握了其活动规律与地形、气温、土壤湿度等生态因子变化的关系,同时还探讨了东北鼢鼠的雌雄比例,繁殖情况、洞道结构和食性等,为今后防治工作提供了科学依据。