黄河流域传统果园发展现状及旅游发展潜力分析

全国重要农业文化遗产系统构成了令人叹为观止的优美人文景观,展现着传统农业生态多样性、生态系统适应性和珍贵的文化遗产。这些世代相传的农业系统构成了当代和未来农业创新和技术的基石。已公布的37个传统果园类全国重要农业文化遗产地,有14个沿黄河流域分布,是我国果业重要的发源地。拟以黄河流域的传统果园为研究对象,系统梳理其发展历史、现状及传统果园农耕系统,同时重点分析黄河流域主要果品的区位发展优劣势,最后结合传统果园的人文价值和区域经济发展因素等,综合探索传统果园的传承和利用途径,为我国果业发展提供传统农业系统和技术创新基础,也为传统果园的保护和传承发展以及农业管理部门挖掘和健全果园文化遗产保护机制提供参考。     

4种黄酒基本营养成分的检测与比较

摘要:目的：为了开发灵芝黄酒,并提高南阳黄酒的知名度,本文测定了绍兴黄酒、南阳黄酒、和两种灵芝黄酒中基本营养元素的含量,并对其含量进行了比较。方法：用氨基酸自动分析仪对四种黄酒中的17种氨基酸进行了全面的定量分析;利用荧光法对四种黄酒中的维生素B1、维生素B2进行了定量分析;采用2,4-二硝基苯肼法检测不同黄酒中的维生素C的含量。结论：四种黄酒在基本营养成分含量上各有所长。     

Molecular cloning and functional analysis of duck Toll-like receptor 5

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.     

激素与营养液配施对平菇产量及品质的影响

植物激素与营养液配合施用可促进平菇菌丝生长和子实体发育，提高产量，改善商品性状，但却不能增加干物质的积累。对营养成分含量的影响随激素与营养液配方的不同而异。     

The role of ectomycorrhizal fungi in alleviating pine decline in semiarid sandy soil of northern China: an experimental approach

The decline of Mongolian pine (Pinus sylvestris var. mongolica) trees on sandy land in northern China has caused serious ecological concerns. Mongolian pine is an ectomycorrhizal fungus (ECM)-dependent species. Three ECM species (Boletus sp., Lactarius deliciosus and L.sp.) were collected from Mongolian pine plantation stands to test their beneficial effects on Mongolian pine seedlings and their responses to environmental factors such as pH, drought stress and temperature. The results indicated that ECM inoculation significantly increased the rate of ECM colonization and the length of seedling shoots. The three ECM could grow in a pH range from 4 to 7, but did not grow under heavy drought stress (?1.53 MPa). High temperatures (over 37 °C) caused death of ECM. When related to soil pH, soil water content and temperature in the Mongolian pine plantations, water conditions and temperature were unfavorable for ECM growth in surface soil, but suitable in deeper soil. Therefore, it was concluded that the failure of natural regeneration in Mongolian pine plantations might be influenced by the lack of ECM in the surface soil because of high temperatures causing ECM death. Moreover, the majority of the root area is distributed in deeper soil, which alleviates the stresses on ECM development and arrests pine decline; this is beneficial for tree growth.     

白牦牛早期胚胎发育条件的初步研究

研究了卵母细胞质量和体外受精卵培养温度对白牦牛早期胚胎发育的影响。结果表明:白牦牛体外受精时要选择A级质量的卵母细胞,体外受精胚胎发育的最适温度为38℃。为完善白牦牛胚胎的体外生产体系提供了实验依据。     

高温胁迫对无柄小叶榕光合作用的影响

以无柄小叶榕为材料,研究了不同时间高温处理对无柄小叶榕气体交换参数、叶绿素荧光参数和Rubisco活力的影响.结果表明,随着高温处理的持续,净光合速率(Pn)持续下降,而气孔导度(Gs)下降非常缓慢,蒸腾速率(Tr)则先开始快速上升随后保持稳定,但是胞间CO2浓度(Ci)却没有明显变化.进一步研究发现,在高温胁迫下,开放PSII反应中心激发能捕获效率(Fv''/Fm'')、光化学猝灭(gP)、PSⅡ实际量子产额(Φ<,PSⅡ>)和电子传递速率(ETR)都持续下降.Rubisco初始活力下降速度则快于这些荧光参数的变化.非光化学猝灭(NPQ)14是先迅速上升后来略有下降.这些结果表明高温抑制无柄小叶榕的光合作用不是由于气孔因素引起的,而是由于高温抑制了Rubisco活力,进而影响PSⅡ活力和电子传递.