浅谈自然保护区在湖北生态建设中的地位与作用

随着全国野生动植物保护和自然保护区建设工程的实施 ,自然保护区在我省生态环境建设中的地位与作用越来越重要 ,运用生物多样性管护区的理论指导我省的自然保护区建设将是正确认识自然保护区地位与作用的关键所在。     

反算方位角在计算机编程中的新算法

本文作者根据自己多年的工程实践,对传统的编程方法和现代的编程方法进行了分析,对比并且得出结论。     

Cytoplasmic diversity of the cotton genus as revealed by chloroplast microsatellite markers

The diversity of chloroplast genomes has played an important role, as have those of nuclear and mitochondrial genomes, in the evolution of plants. The sequences of the chloroplast genome supply unsubstituted information for genome analysis. In order to understand the genetic differentiation and relationship of cotton species, we investigated the cytoplasmic diversity of chloroplast genomes in 41 Gossypium accessions with 75 chloroplast simple sequence repeat (cpSSR) markers. The markers were developed from reference sequences of the chloroplast genomes of G. hirsutum and G. barbadense and covered approximately 12.6 kb. Among the 75 markers, 50 were polymorphic, with polymorphism information content values ranging from 0.11 to 0.88. Analyses of the dataset demonstrated that single copy regions were much more informative than inverted repeat regions. The non-coding sequences were well differentiated among these species. For some common cpDNA haplotypes, the E-genome species that may be the oldest of the extant cotton species was deduced. The differentiation of A-genome species lagged behind that of AD-genome species. Neither G. herbaceum nor G. arboreum was the cytoplasmic donor of tetraploid species, strongly suggesting that AD genomes originated from an extinct ancestor of modern A-genome species. We speculate that the genetic differentiation of the chloroplast genome of each cotton species resulted from the dispersal of that species and its adaptations to local ecological conditions. These cpSSR markers provided valuable information to reveal the diversity and differentiation of cotton during evolution.     

采摘机器人位置校正方法研究——基于无线传感网络和超带宽调频方法

超带宽雷达是新近出现的一种高分辨率雷达,当信号带宽和其发射中心频率的比大于0.25时,就被称作超带宽,带宽越大,雷达对目标的分辨能力越高。超带宽雷达具有高分辩能力,如果将其使用在采摘机器人定位导航系统中,可以有效地提高采摘机器人的定位导航精度和效率。为此,提出了一种基于无线传感网络和超带宽调频技术的采摘机器人位置校正方法,并以实际采摘环境作为研究对象,对采摘机器人的位置校正过程进行了测试。测试结果表明:采用超带宽调频无线传感网络方法,定位校正精度要高于传统的定位算法,且定位效率高,对于新型自动化采摘作业机器人的研究具有重要的指导意义。     

微生物菌肥与化学肥料配施对盈江省藤生长的影响

为了解不同肥料种类及其施肥量对盈江省藤生长的影响,采用L₉(3⁴)正交试验设计对其2年生幼林进行连续3年的微生物菌肥与复合肥和磷肥配施试验。结果表明,3年中不同因素的各水平组合间苗木的地径、株高、叶片数和冠幅均呈现极显著的差异(P<0.01)。施用复合肥和磷肥总体呈现显著(P<0.05)或极显著(P<0.01)地抑制盈江省藤生长的现象。不同肥料配施试验组合的地径、株高和叶片数均大于对照的相应指标。试验期间,单施微生物菌肥0.3~0.6 g/株即可满足盈江省藤生长的需求。     

基于FSEC赛车电池箱的结构设计与强度分析

以中国大学生电动方程式赛车的电池箱体研究为背景,对电池箱的结构设计和强度分析方面进行了研究论证,提出了电池箱设计的总体思路,使电池箱的结构设计紧凑合理,工作安全可靠,符合赛事规则。这对纯电动乘用车在电池箱的结构设计方面也具有参考和借鉴的价值。     

危难时刻,训练爱犬救你

犬是最忠于人类的动物。在带犬执行任务中，犬主人经常面临突遭袭击或袭击后的危险，针对这一问题，要培养犬良好的应变能力，培养其在突发事件中护主救人的能力，综合发挥犬的特殊作用。     

Involvement of Sirt1 in mouse myocardial hypertrophy induced by isorenin

AIM: To investigate the expression and function of sirtuin 1 (Sirt1), one of the class III histone deacetylases, in hypertrophied mouse myocardium induced by isorenin (ISO). METHODS: The Kunming mice were randomly divided into 3 groups: control group, ISO group and ISO+nicotinamide (NAM) group. The myocardial hypertrophy was induced by dorsal subcutaneous injection of isorenin. Nicotinamide, an inhibitor against Sirt1, was given by peritoneal injection. Heart weight index (heart weight/body weight), hematoxylin and eosin staining, transmission electron microscope and mRNA expression of brain natriuretic peptide (BNP) were observed to identify the myocardial hypertrophy. The expression of Sirt1 in mRNA and protein levels was detected by RT-PCR and Western blotting, respectively. RESULTS: Compared to control group, the results of heart weight index, hematoxylin and eosin staining, the observation of transmission electron microscopy and mRNA expression of BNP showed that the mouse myocardial hypertrophy was induced by isorenin successfully. The Sirt1 expression was increased in hypertrophy model group (P<0.01 vs control group). Treatment with nicotinamide inhibited the cardiac hypertrophy induced by ISO (P<0.05 vs ISO group) and decreased the expression of Sirt1 (P<0.01 vs ISO group). CONCLUSION: Activation of Sirt1 might be involved in the process of myocardial hypertrophy stimulated by isorenin in mice.     

矾根主要品种的叶色表型性状分析

通过目测进行分类的方式很难准确界定矾根品种叶色。为建立一套更为科学的基于叶色表型的矾根品种分类体系,笔者利用色差仪对72个矾根品种共计432个样品的叶色进行测定,对获得的L、a、b 3个色度值进行聚类分析,将72个矾根品种分为褐色系、深绿色系、黄色系、灰绿色系、浅绿色系5类色系。对矾根品种各色系的L、a、b值进行相关性分析,发现叶片正面的L值与a值、a值与b值呈负相关,L值与b值呈正相关,但相关性不明显;叶片背面的L值与a值、a值与b值呈负相关,L值与b值呈显著正相关。从多重比较的结果中可以看出,矾根品种各色系之间的L、a、b色度值差异显著,尤其是各色系间叶正面的颜色差异较大,在矾根品种选育工作中,叶片正面的颜色将作为主要参考。     

孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响

为了明确ABA对孕穗期低温下水稻结实率及叶片生理特性的调控效应,本试验在盆栽条件下,以2个水稻(Oryza sativa L.)品种‘龙粳11’（冷敏型）和‘龙稻5’（耐冷型）为材料,于孕穗期在人工气候室进行低温（15℃,持续1,2,3,4,5天）处理,并喷施不同浓度的脱落酸(ABA),研究低温条件下ABA对不同水稻结实率的调控效应,同时探讨了ABA和低温对水稻叶片膜透性、抗氧化酶等生理特性的影响。结果表明：低温条件下,‘龙稻5’的结实率变化不大,‘龙粳11’的结实率显著降低,20 mg/L的ABA能够有效缓解低温伤害,相对提高低温胁迫下水稻的结实率。叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性表现出先升高后下降的变化趋势,‘龙稻5’的抗氧化酶活性、可溶性糖和可溶性蛋白含量高于‘龙粳11’,但丙二醛(MDA)含量和相对电导率低于‘龙粳11’。ABA可提高冷敏品种的抗氧化酶活性、可溶性糖和可溶性蛋白的含量,同时可降低MDA含量和相对电导率。这说明ABA可以通过调控水稻抗氧化酶、可溶性糖等生理指标的活性及含量,进而提高冷敏水稻品种的耐冷性。