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961.
Determination of the potassium balances in diary cows and the examination of daily and lactation period-associated variations 下载免费PDF全文
Sattler N Fecteau G Couture Y Tremblay A 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(2):107-115
The objectives of this study were to evaluate a method of determining the internal and external potassium balances in dairy cows and to determine daily and lactation period-associated variations of these balances. Ingested potassium, milk potassium concentration (LK) and potassium urinary fractional clearance (FeK) defined the potassium external balance. Serum (SK) and red blood cell (CIEK) potassium concentrations defined the potassium internal balance. Whole blood, urine, and milk were collected at different times of the day from 19 cows, grouped in 5 lactation periods. Significant differences were observed for LK between lactation periods. Significant variations occurred during the day for CIEK, LK, and FeK. Thus, in order to monitor potassium balances during a time period, samples should be collected at the same time each day. This method of determining both potassium balances simultaneously could be useful in the study of the bovine hypokalemia syndrome. 相似文献
962.
1. The perception of UVA light (320 < lambda < 400 nm) by domestic fowl may be an adaptation to naturally lit habitats and may still be functional in farmed varieties, although not utilised owing to the absence of these wavelengths in commercial house lighting. One function may be the mediation of sexually related signalling or communication. 2. In experiment 1, two groups of 41 adult broiler breeders (four cockerels, 37 hens) were kept under conventional fluorescent light, with or without fluorescent UVA supplementation amounting to 16.9% of the total spectral power output of the luminaires. Each light environment was approximately iso-illuminant as perceived by the birds. The two groups were exposed to the light environments alternately for five 2-day periods in a cross-over design. Mating behaviour, production measures and time budgets were recorded on the second day of each period. 3. A UVA-enriched environment increased the number of attempted matings (1.27 vs 0.99 matings/cockerel.h) and locomotion (5.3 vs 3.7 min/bird.hour). 4. In a second experiment, 10 hens were allowed to choose between four cockerels lit under different power levels of UVA (1.6%, 14.6%, 43.5%, 57.5% of the total spectral power output of the luminaires) in a four-armed maze. Again, each light environment was approximately iso-illuminant as perceived by the birds. Each hen was allowed to make one choice per day over four days, with the position of the cockerels and the UVA levels interchanged each day. This schedule was repeated with the same hens for two other groups of four cockerels. 5. At a distance of no less than 60 cm from the cockerel, the hens spent most time inspecting whichever cockerel was lit by 1.6% or 14.6% UVA (1.33 vs 1.37 vs 1.22 vs 1.16 log seconds/hen.choice, respectively for increasing UVA level). Similarly, when allowed to approach closer to the cockerels, the hens spent most time in the arm which contained a cockerel lit by 14.6% UVA light, (1.62 vs 1.88 vs 1.69 vs 1.51 log s/hen.choice, respectively for increasing UVA level). 6. UVA is clearly implicated in the transmission of sexual signals or communication which may have implications for the welfare and production of broiler breeders. 相似文献
963.
Mycoplasma synoviae (MS) was isolated from a flock of commercial tom turkeys in which a small percentage of the birds exhibited clinical signs and lesions typical of MS synovitis. However, serologic testing of such flocks revealed poor to inconsistent reactivity by agglutination, enzyme-linked immunosorbent assay (ELISA) or hemagglutination inhibition; isolation of MS from such flocks proved to be very difficult. Turkeys were challenged with one of the isolates (K4463B) either by aerosol or systemically by a combination of intravenous, foot pad, and eyedrop routes. Turkeys challenged by the systemic route responded normally to all serologic tests, whereas those challenged by aerosol either responded very poorly on all serologic tests or were seronegative up to 6 wk postchallenge even though they were positive for MS by tracheal culture. These results suggest that turkeys may harbor an upper respiratory infection with MS while remaining serologically negative. 相似文献
964.
The objective of this study was to determine the effect of varying concentrations of dietary fat and carbohydrate on changes in body composition of Holstein bull calves fed under isocaloric and isonitrogenous intake conditions. Thirty-two calves were assigned to a randomized block design with three dietary treatments, eight calves per treatment, and one baseline group of eight calves. Animals were reared from birth to 85 kg live BW (SEM = 0.57). All calves began treatments between 2 and 6 d of age. Three different milk replacer treatment diets were designed to deliver 14.8 (low fat, LF), 21.6 (medium fat, MF), or 30.6% of DM (high fat, HF) fat; 55.3, 46.7, or 35.4% of DM lactose; and 23.5, 24.8, or 27.0% of DM CP, respectively. Gross energy values were 4.62, 5.09, and 5.77 Mcal/kg for the LF, MF, and HF milk replacers, respectively. From d 1 to 14, calves were offered 0.24 Mcal intake energy/kg BW(0.75), adjusted weekly based on increases in BW, and 0.28 Mcal intake energy/kg BW(0.75) from d 15 to slaughter. Dry feed was not offered. Dry matter, energy, CP, and fat intakes were 55.2 kg, 257.6 Mcal, 13.0 kg, and 8.2 kg; 52.8 kg, 268.8 Mcal, 13.1 kg, and 11.4 kg; and 46.8 kg, 270.3 Mcal, 12.6 kg, and 14.3 kg for the LF, MF and HF treatments, respectively. Energy and CP intakes did not differ among treatments (P = 0.63 and 0.79, respectively). Fat and DMI were different among treatments (P = 0.001 and 0.02, respectively). Empty BW gains were 0.61, 0.61, and 0.65 kg/d for LF, MF, and HF, respectively, and were not different among treatments (P = 0.27). Empty body CP, water, and ash gain were not different among treatments (P = 0.65, 0.99, and 0.57, respectively). Empty body retained energy and fat gain were 27.2 and 57.7% greater for calves fed the HF than for those fed the LF diet (P = 0.06 and 0.006, respectively). Fat as a percentage of the whole empty body on a water-free basis was lower for calves consuming the LF diet (28.6%) than for those fed the HF diet (34.6%), whereas percentage of CP on an empty body, water-free basis was greater for calves consuming the LF diet (59.2%) than for those fed the HF diet (54.9%) (P = 0.006). The results of this study demonstrated that, under isocaloric and isonitrogenous intake conditions, equivalent dietary energy from fat compared to carbohydrate, above 15% fat, has no beneficial purpose unless additional fat deposition is required in the animal. Further, the data demonstrate significant changes in body composition independent of growth rate. 相似文献
965.
Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro 总被引:4,自引:0,他引:4
Wallace RJ Wallace SJ McKain N Nsereko VL Hartnell GF 《Journal of animal science》2001,79(7):1905-1916
This study was done to determine the effectiveness of supplementary enzymes at increasing the fiber digestion by ruminal microorganisms and to assess whether enzyme activity limits the rate of fiber digestion in ruminal digesta. In vitro comparisons of enzyme activities in two feed enzyme preparations (A and B) with enzyme activities extracted from ruminal fluid indicated that the addition of fibrolytic enzymes at the application rates recommended by the manufacturers would not be expected to increase significantly glycanase and polysaccharidase activities in ruminal fluid. Preparations A and B both increased (P < 0.001) the rate of gas production from freeze-dried corn and grass silages in in vitro incubations with ruminal fluid, but only at concentrations much higher than recommended application rates. Autoclaved controls had little or no effect. Ultrafiltration of enzyme B indicated that most stimulation was due to components >100 kDa, which is consistent with the cause of the stimulation being enzyme activity. Fibrolytic enzymes from other sources were also able to stimulate gas production: increased rates of gas production were observed in seven out of eight combinations of "cellulase" and corn or grass silage (P < 0.05). The comparison of glycanase and polysaccharidase activities with gas-stimulatory activity in the different enzyme preparations indicated that the highest correlation was between increased gas production and enzyme activity against microgranular cellulose (P < 0.05). In a wider range of fibrolytic enzyme preparations, those with endo-(beta-1,4)- or exo-(beta-1,4)-xylanase activity equal to that of preparation A did not produce similar increased rates of fermentation of corn silage when glucanase activity was low (P > 0.05). In contrast, preparations with glucanase activity similar to enzyme A gave at least as great (P < 0.05) an improvement in gas production than enzyme A, irrespective of xylanase activity. It was concluded that enzyme activity, probably a type of endo-(beta-1,4)-glucanase activity, limits the rate of fermentation of corn and grass silage in the rumen. Enzyme supplements of the type used in these experiments are unlikely to possess sufficient activity to overcome this limitation by direct application to ruminal digesta, implying that treatment of the ration prefeeding will be key to harnessing the potential of exogenous fibrolytic enzymes in ruminant nutrition. 相似文献
966.
Stöcklin-Gautschi NM Guscetti F Reichler IM Geissbühler U Braun SA Arnold S 《The Journal of small animal practice》2001,42(8):413-416
A focal uterine adenomyosis is described in two bitches. In both cases, the uterus showed knobbly enlargements of 4 to 8 cm in diameter, which resulted in distinct clinical symptoms. Other pathological changes of the uterus were not present. One bitch was presented because of a history of vaginal discharge of several months' duration. Radiographs, as well as ultrasonography, revealed a soft tissue lesion at the cervix. The other bitch showed a marked reduction in its general condition and a sudden onset of a tense abdomen. Radiologically, a lesion of soft tissue opacity was observed in the mid-abdomen and was seen to originate from the left uterine horn during exploratory laparotomy. A torsion of the lesion was present, which explained the clinical signs in this second case. 相似文献
967.
Effect of K-diformate in starter diets on acidity, microbiota, and the amount of organic acids in the digestive tract of piglets, and on gastric alterations 总被引:17,自引:0,他引:17
The aim of this investigation was to study the effect of K-diformate on the intraluminal pH, microbial composition in digesta and feces, organic acids along the digestive tract, and alterations of the gastric epithelium of pigs. Pigs (n = 36) weaned at 28 d of age were allotted to two groups and fed without (control diet) or with 1.8% supplemental K-diformate. Fecal samples were taken from the rectum on d 0, 1, 3, 5, 7, 14, 21, and 28 postweaning. Half of the animals from each group were killed on d 7 and the other half on d 29 postweaning. Growth performance was not different for both groups (P > or = 0.73). The gastric epithelium was not negatively affected by K-diformate (P = 0.25). Potassium-diformate decreased (P < or = 0.04) or tended to decrease (P < or = 0.10) the counts of total anaerobic bacteria, lactic acid bacteria, and yeasts in feces and digesta samples from the stomach, distal small intestine, cecum, and middle segment of the colon. The pH along the gastrointestinal tract of piglets was not affected by K-diformate (P > or = 0.30). On d 7, the concentration of lactic acid along the gastrointestinal tract was similar with both diets (P = 0.15). On d 29, the concentrations of lactic acid tended to be lower along the small intestine (P < or = 0.08) and the stomach (P = 0.11) of the pigs fed K-diformate. Formic acid in digesta was detected at significant levels only in the distal segment of the small intestine of the control pigs (from 4 to 11 mmol/kg of wet digesta), whereas considerable amounts were measured in the stomach (from 23 to 40 mmol/kg of wet digesta) and all segments of the small intestine (from 7 to 25 mmol/kg of wet digesta) in the K-diformate-fed pigs on both days. On d 7, pigs fed the K-diformate diet had a tendency (P < or = 0.08) to have higher concentrations of organic acids (acetic + propionic + butyric) in the digesta of the distal small intestine, cecum, and proximal colon. On d 29, both groups had similar concentrations of these acids, irrespective of the segment of the gastrointestinal tract (P = 0.95). Our study showed that the addition of K-diformate to a starter diet for piglets decreased total anaerobic bacteria, lactic acid bacteria, coliforms, and yeasts in feces and in digesta from various segments of the gastrointestinal tract, without affecting the gastric or intestinal pH. 相似文献
968.
Kristensen NB 《Journal of animal science》2001,79(9):2491-2498
To investigate the impact of rumen microbial sequestration of VFA carbon on estimates of acetate availability based on intraruminal infusion of [2-(13)C] acetate, three nonlactating or low-yielding dairy cows were continuously intraruminally infused with [2-(13)C]acetate for 26 h. The 13C content of ruminal VFA, duodenal carbon, and fatty acids (FA) and AA isolated from liquid-associated ruminal microbes and duodenal DM was measured by an isotope ratio mass spectrometer interfaced to an elemental analyzer or a gas-liquid chromatograph. The ruminal gross production of acetate was 38 +/- 4 mol/d and could account for about 38% of the DE intake. Of the intraruminally infused 13C in [2-(13)C]acetate, 7.6 +/- 0.9% was recovered at the duodenum. The 13C content of ruminal propionate, butyrate, and valerate increased (P < 0.05) with intraruminal infusion of [2-(13)C]acetate. It was estimated that about 28% of the 13C intraruminally infused in [2-(13)C]acetate could be accounted for by duodenal 13C flow and absorption of non-acetate VFA. A number of FA isolated from liquid-associated ruminal microbes (C6, C12, C14, anteiso C15, and iso C15) were enriched with 13C (P < 0.05) at a level comparable to the enrichment of ruminal butyrate. Any absorption of these FA from the rumen would further contribute to non-acetate 13C uptake. A maximum of 72% of the ruminal gross production of acetate represented acetate absorption from the rumen in the present study. Consequently, previously used models using intraruminal isotope dilution techniques seem not to be appropriate for measuring acetate availability in ruminants. The number of metabolites exchanging carbon with acetate was found to be so high that assessments of the entire range of inter conversions seem to be practically impossible. Portal absorption studies are discussed as an alternative method of estimating VFA availability to the metabolism in ruminants. 相似文献
969.
Nsoso SJ Machete JB Molatole M Ndebele RT Lebani NN Chabo RG Kalake AM Jacyna L Segadimo BW Mine OM 《The Onderstepoort journal of veterinary research》2001,68(2):101-104
Data collected monthly over a period of two years were live weight, packed cell volume (PCV), nematode faecal egg counts (FECs) and coccidial oocyst counts from faecal analyses for 100 mixed age (3-7 years) indigenous Tswana does. The aims of this experiment were to determine seasonal FECs and coccidial oocysts in these goats and quantify the relationships of these burdens to liveweight and PCV. FECs significantly (P < 0.05) varied with season, with the warmer seasons viz spring, summer and autumn having higher log (x + 1) parasite burdens than the cooler winter, while seasonal trends for coccidial oocysts were not obvious. PCV was also significantly (P < 0.05) lower in the warmer seasons than winter. FECs and coccidial oocysts in all seasons were less than the mean log (x + 1) of 3.3 inferred to reduce production in small stock. Correlation coefficients were strongly negative: -0.95 for FECs and liveweight and -0.84 for FECS and PCV, indicating that these worms had a negative impact on productivity. A further study should be conducted to quantify the effects of controlling these parasites during the warm seasons on productivity. 相似文献
970.
Schultz RA Fourie N Basson KM Labuschagne L Prozesky L 《The Onderstepoort journal of veterinary research》2001,68(4):325-330
Pavetamine, the active principle of plants causing gousiekte in ruminants, was found in this study to be an inhibitor of protein synthesis in the rat heart. Sprague-Dawley rats were injected intra-peritoneally with 8-10 mg/kg pavetamine and the levels of protein synthesis in different organs determined utilizing L-[4-3H]phenylalanine incorporation. In contrast to the more than 23% inhibition found in heart tissue at 4, 24 and 48 h after administration of pavetamine, the effect on the kidney, liver, spleen, intestine and skeletal muscle was minimal or returned to pretreatment levels within 48 h. These results may offer an explanation for the clinical signs observed in ruminants with gousiekte, where the heart only is affected. 相似文献