Diversity of the human intestinal microbial flora

The human endogenous intestinal microflora is an essential "organ" in providing nourishment, regulating epithelial development, and instructing innate immunity; yet, surprisingly, basic features remain poorly described. We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity. A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms. We discovered significant intersubject variability and differences between stool and mucosa community composition. Characterization of this immensely diverse ecosystem is the first step in elucidating its role in health and disease.     

Sex-Reversal of Oreochromis aureus by Immersion in Mibolerone,a Synthetic Steroid 1

Mibolerone (MI), a synthetic steroid, was used to sex-reverse undifferentiated Oreochromis aureus fry. Fry were exposed to 0.0, 0.3, 0.6, or 1.0 ppm MI in static-water solutions for five weeks (immersion treatments) or were fed a diet containing 1.0 ppm MI for four weeks in a flow-through system. Following hormone treatment, the fish were grown to over 60 mm total length prior to sex determination using gonadal squash examination. Fish immersed in 1.0, 0.6, or 0.3 ppm MI had average tissue MI concentrations of 14.4, 5.6, and 3.3 ppm, respectively. Immersion in either 1.0 or 0.6 ppm MI for five weeks resulted in an average of 82% males and 18% ovo-testicular fish (inter-sex fish) with no gonadal females being produced. Exposure to 0.3 ppm MI resulted in 78.7% males, 20.7% ovo-testicular fish, and 0.7% gonadal females. Feeding a diet containing 1.0 ppm MI resulted in 85% males, 11% ovo-testicular fish, and 4% gonadal females. Fry growth and survival were negatively correlated with the MI concentration of the immersion treatments. Exposing tilapia fry to static-water solutions of 0.6 ppm MI for five weeks appears to be a feasible method of eliminating the production of functional females. Immersion solutions should be changed at least weekly to maintain an effective hormone concentration.     

Rapport General

Sans résumé     

Food insecurity among homeless and runaway adolescents

OBJECTIVE: The purpose of this study was to investigate the prevalence of food insecurity and factors related to it among homeless and runaway adolescents. DESIGN: Computer-assisted personal interviews were conducted with homeless and runaway adolescents directly on the streets and in shelters. SETTING: Interviews were conducted in eight Midwest cities: Des Moines, Cedar Rapids, Iowa City, Kansas City, Lincoln, Omaha, St. Louis and Wichita. SUBJECTS: The subjects were 428 (187 males; 241 females) homeless and runaway adolescents aged 16-19 years. Average age of the adolescents was 17.4 (standard deviation 1.05) years. RESULTS: About one-third of the adolescents had experienced food insecurity in the past 30 days. Factors associated with food insecurity were age of adolescent, a history of caretaker neglect and abuse, having ever spent time directly on the street, a small post-runaway social network, and engaging in deviant and non-deviant street food-acquisition strategies. CONCLUSIONS: Based on these findings, our conservative estimate is that nationally more than 165,000 homeless and runaway adolescents experienced food insecurity in the past 30 days. These adolescents are largely hidden from public notice and they are usually missed in studies that address national hunger.     

Effectiveness of insecticide-treated and non-treated trap plants for the management of Frankliniella occidentalis (Thysanoptera: Thripidae) in greenhouse ornamentals

The effectiveness of trap cropping as an integrated control strategy against western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), was explored in potted chrysanthemum, Dendranthema grandiflora (Tzvelev), greenhouse crops. The efficacy of flowering chrysanthemum trap plants, either treated with the insecticide spinosad or untreated, to regulate F. occidentalis populations was tested at different spatial scales (small cage, large cage and commercial greenhouse) and for different time periods (1 or 4 weeks). It was demonstrated that flowering chrysanthemums as trap plants lower the number of adult F. occidentalis in a vegetative chrysanthemum crop and, as a result, reduce crop damage. In the 4 week large-cage trial and the commercial trial, significant differences between the control and the trap plant treatments started to appear in the third week of the experiment. Larvae were only significantly reduced by the presence of trap plants in the 1 week small-cage trials. There were no significant differences between treatments with spinosad-treated and untreated trap plants in the number of F. occidentalis on the crop. This suggests that there was minimal movement of adult F. occidentalis back and forth between the trap plants and the crop to feed and oviposit. It is concluded that the trap plant strategy is a useful tool for integrated pest management against F. occidentalis in greenhouses.     

Comparison of a novel distillation method versus a traditional distillation method in a model gin system using liquid/liquid extraction

This research studied a novel form of distillation (high vacuum distillation) as a method for preserving volatile aroma chemicals important to the organoleptic attributes of a four botanical model gin as well as the degradation products generated during the heating required in traditional methods of gin distillation. A 2 (5) factorial experiment was conducted in a partially confounded incomplete block design and analyzed using the PROC MIXED procedure from SAS. A model gin was made of dried juniper berries (Juniperus communis), coriander seed (Coriandrum sativum), angelica root (Angelica archangelica), and dry lemon peel (Citrus limonum). This was distilled on a traditional still utilizing atmospheric pressure and a heating mantel to initiate phase separation as well as a novel still (high vacuum) utilizing high vacuum pressures below 0.1 mmHg and temperatures below -15 degrees C to initiate phase separation. The degradation products (alpha-pinene, alpha-phellandrene, E-caryophyllene, and beta-myrcene) were present at greater levels (approximately 10 times) in the traditional still-made gin as compared to the novel gin.     

Long-term survival of de-oiled Cape gannets Morus capensis after the Castillo de Bellver oil spill of 1983

After oil spills, oiled wildlife are regularly cleaned at considerable cost. Yet the conservation value of this intervention has been questioned, mostly because cleaned animals appear to have poor post-release survival. However, reliable long-term data are needed to judge the success of such programs. We used 16 ring recoveries to estimate survival of 932 Cape gannets (Morus capensis) that were oiled, cleaned and released in 1983. For the period 1989-2006, we further compared survival of 162 surviving cleaned gannets to that of 10,558 non-oiled gannets using capture-recapture data. We used modern statistical tools that account for recapture probabilities, recovery rates, transience, and temporary absence from the breeding colonies. Mean annual survival rates of de-oiled gannets ranged from 0.84 (se = 0.05) to 0.88 (se = 0.02), depending on analysis and colony. Between 1989 and 2006, rehabilitated gannets survived slightly less well than unoiled birds, but the difference was similar to the difference in survival between the two colonies where the birds were studied. Our results show subtle long-term effects of oiling and subsequent treatment. However, they also show that cleaned gannets can survive at almost the same rate as unoiled birds, at least if they survived the initial years after release. Rehabilitation of these birds may thus be a valuable conservation intervention for this localised species where a single spill can threaten a large proportion of the world population.     

Prediction of atrazine sorption coefficients in soils using mid-infrared spectroscopy and partial least-squares analysis

This study explored the potential of mid-infrared spectroscopy (MIR) with partial least-squares (PLS) analysis to predict sorption coefficients (Kd) of pesticides in soil. The MIR technique has the advantage of being sensitive to both the content and the chemistry of soil organic matter and mineralogy, the important factors in the sorption of nonionic pesticides. MIR spectra and batch Kd values of atrazine were determined on a set of 31 soil samples as reference data for PLS calibration. The samples, with high variability in soil organic carbon content (SOC), were chosen from 10 southern Australian soil profiles (A1, A2, B, and C in one case). PLS calibrations, developed for the prediction of Kd from the MIR spectra and reference Kd data, were compared with predictions from Koc-based indirect estimation using SOC content. The reference Kd data for the 31 samples ranged from 0.31 to 5.48 L/kg, whereas Koc ranged from 30 to 680 L/kg. Both coefficients generally increased with total SOC content but showed a relatively poor coefficient of determination (R2 = 0.53; P > 0.0001) and a high standard error of prediction (SEP =1.22) for the prediction of Kd from Koc. This poor prediction suggested that total SOC content alone could explain only half of the variation in Kd. In contrast, the regression plot of PLS predicted versus measured Kd resulted in an improved correlation, with R2 = 0.72 ( P > 0.0001) and standard error of cross-validation (SECV) = 0.63 for three PLS factors. With the advantages of MIR-PLS in mind, (i) more accurate prediction of Kd, (ii) an ability to reflect the nature and content of SOC as well as mineralogy, and (iii) high repeatability and throughput, it is proposed that MIR-PLS has the potential for an improved and rapid assessment of pesticide sorption in soils.     

Diversity of the composition and content of soluble carbohydrates in seeds of the genus <Emphasis Type="Italic">Vicia</Emphasis> (Leguminosae)

Low molecular weight carbohydrates of seeds of 10 species of Vicia, namely: V. angustifolia, V. articulata, V. cordata, V. ervilia, V. johannis, V. macrocarpa, V. monantha, V. narbonensis, V. pannonica and V. sativa were analyzed by the high resolution gas chromatography method. Seeds of the investigated species contain common (glucose, fructose, myo-inositol, sucrose, galactinol, di-galactosyl myo-inositol and raffinose family oligosaccharides—RFOs) and species-specific carbohydrates (d-pinitol and its α-d-galactosides—in V. articulata, V. monantha and V. pannonica or d-ononitol and its galactoside—in V. ervilia). Among the species containing in seeds RFOs as the main α-d-galactosides (V. angustifolia, V. cordata, V. johanensis, V. macrocarpa, V. narbonensis and V. sativa), an additional subgroup can be separated, which contains a set of unknown compounds (found in V. angustifolia, V. cordata and V. macrocarpa). Moreover, several other unidentified carbohydrate-containing compounds were detected exclusively in seeds of V. ervilia. The concentrations of total soluble carbohydrates (TSCs), including sugars, RFOs, cyclitols and galactosyl cyclitols and unknown compounds, in seeds differ significantly (P < 0.05) among the species. RFOs and sucrose are the main fractions in TSCs, with the exception of V. pannonica seeds, containing more galactosyl pinitols, and V. ervilia, in which unknown compounds account for 50% of TSCs. In V. ervilia RFOs occur at the lowest concentration (9.32 mg g^?1 seeds), and the concentration of DGMI (di-galactosyl myo-inositol) is comparable with that of RFOs, which is unusual among all the investigated Vicia species.     

Urban forest assessment in Bangkok,Thailand

The objective of this study was to conduct an urban forest assessment for Bangkok and pilot the appropriateness of adapting the i-Tree Eco International software in Thailand. Urban forest structure, function, and value were estimated from 184 stratified random sample plots. A total 48 tree species were encountered and the three most common that contributed 34.1% of total tree population were Polyalthia longifolia Sonn. (15.7%), Mangifera indica L. (13.0%), and Pithecellobium dulce (Roxb.) Benth. (5.4%). The majority of trees (approximately 70%) were <23 cm in diameter. An estimated 2.5 (0.4 SEM) million trees with a canopy cover of 8.6% (1.3 SEM) exist in the Bangkok study area. These trees store an approximate total of 309,700 (73,726 SEM) metric tons of carbon and sequester 16,271 (2965 SEM) metric tons of CO₂ annually. Additional research is needed to develop functional relationships for urban trees in Thailand to overcome a current limitation for using i-Tree Eco in Thailand to estimate structural and functional value of urban forests. Study results also provide land-use planners information on the current urban forest resource and the potential for future urban greening.