Complex interplay between intrinsic and extrinsic drivers of long-term survival trends in southern elephant seals

Background  

Determining the relative contribution of intrinsic and extrinsic factors to fluctuations in population size, trends and demographic composition is analytically complex. It is often only possible to examine the combined effects of these factors through measurements made over long periods, spanning an array of population densities or levels of food availability. Using age-structured mark-recapture models and datasets spanning five decades (1950–1999), and two periods of differing relative population density, we estimated age-specific probabilities of survival and examined the combined effects of population density and environmental conditions on juvenile survival of southern elephant seals at Macquarie Island.     

Technical note: Use of near-infrared reflectance spectroscopy to predict intake and digestibility in bulls and steers

Multiple fecal samples were collected from growing Angus bulls (264 to 419 kg of BW, 3.0 to 11.4 kg/d of DMI) to predict DMI of a corn-silage-based diet. Contemporaneous digestion trials were conducted with the same diet in 12 steers in yr 1 to 3 and bulls in yr 4. Near-infrared spectra from fecal samples (n = 730 from 282 growing bulls, n = 240 from 36 steers and 12 bulls for digestion trials) were obtained from dried and ground fecal samples, and modified partial least squares regression was used to develop equations to predict DMI and DM digestibility (DMD). Although mean predicted DMI of the growing bulls (7.52 ± 0.04 kg/d or 22.4 ± 0.1 g/kg of BW) was within 2% of mean measured DMI (7.63 ± 0.06 kg/d or 22.7 ± 0.1 g/kg of BW), the mean of paired differences within samples (0.11 ± 0.04 kg/d or 0.3 ± 0.1 g/kg of BW) was greater (P < 0.01) than zero. Measured DMD (72.3 ± 0.5%) was identical (P < 0.97) to predicted DMD (72.3 ± 0.5%), and DMD for bulls in the digestion trial did not differ (P < 0.27) from DMD for steers. Prediction of intake requires incorporation of some measured values from the set of fecal samples to be predicted. Lack of similarity between spectra of fecal grab samples from the growing bulls and daily fecal collection of steers and bulls in the digestion trials in this study indicates the need for further verification before prediction of DMD with fecal grab samples.     

Investigation of the heating rate dependency associated with the loss of crystalline structure in sucrose, glucose, and fructose using a thermal analysis approach (part I)

Thermodynamic melting occurs at a single, time-independent temperature with a constant enthalpy value. However, substantial variation in the melting parameters (T(m onset), T(m peak), and ΔH) for sucrose, glucose, and fructose has been reported in the literature. Although a number of explanations have been put forth, they do not completely account for the observed variation. Thus, this research was performed to elucidate the fundamental mechanism underlying the loss of crystalline structure in the sugars using both thermal (Part I) and chemical (Part II) analysis approaches. A strong heating rate dependency observed in the melting parameters for the sugars implies the occurrence of a kinetic process during the loss of crystalline structure. The difference in heat capacity and modulated heat flow amplitude in the stepwise quasi-isothermal modulated differential scanning calorimetry experiments for the sugars compared to indium and mannitol (thermodynamic melting comparison materials) strongly suggests thermal decomposition as the kinetic process responsible for the loss of crystalline structure, which is the critical difference between our conclusion and others. We propose the term "apparent melting" to distinguish the loss of crystalline structure due to a kinetic process, such as thermal decomposition, from thermodynamic melting.     

Rabbiteye blueberry plant response to nitrogen and phosphorus

Acid, sandy soils selected for blueberry production are usually deficient in plant available phosphorus. Fertilizer addition at planting has not been a recommended practice. This research was designed to evaluate potted blueberry (Vaccinium ashei Reade) plant growth response to P applied at establishment and interacted with N applied during the growing season. Four liter containerized ‘Tifblue’ rabbiteye blueberry plants were transplanted into 4.4 kg of 1:1 v/v loamy sand:peat mixture in an 8‐liter container. Preplant P was incorporated into the mixture at 0, 20, 40, 60, and 80 kg/ha rates. Nitrogen was split‐applied post‐plant four times at 60‐day intervals beginning 30 days after blueberry bushes were transplanted. Total N rates were equivalent to 0, 112, 224, or 336 kg/ha. Plants were harvested 270 days after transplanting. Phosphorus at the 60 and 80 kg/ha rates increased plant growth 40 and 31%, respectively. Plant growth responded linearly to increasing N rate. Leaf and stem weight increases contributed to this linear response while root weight was not affected by fertilizer N. Total plant dry weight was increased by N rates at the 60 and 80 kg/ha P levels. Phosphorus applications increased leaf P levels, but did not increase soil test P. Leaf redness, caused by cool temperatures, was reduced by high N rates. The optimum P rate for fertilization of the soilrpeat mixture in the planting hole under conditions of this experiment was the equivalent of 60 kg/ha.     

Krankheitsursachen und Schädlingsbekämpfung

Ohne Zusammenfassung     

Heterozygote detection for maple syrup urine disease in cattle

SUMMARY The clinical, pathological and biochemical manifestations of maple syrup urine disease (MSUD) are similar in Poll Hereford and Poll Shorthorn X Poll Hereford calves. No significant differences were observed in branched-chain amino acid concentrations in plasma, or of branched-chain keto acid dehydrogenase activity in fibroblasts, between Poll Herefords homozygous normal and heterozygous for the mutation responsible for MSUD. Haemopoietic chimerism resulted in incorrect diagnosis of the MSUD genotype in 30% of non-identical twins when blood DNA was analysed using allele-specific amplification. Hair roots are shown to be a suitable source of target DNA for genotyping Poll Hereford cattle for the MSUD mutation. Twelve of 203 (5.8%) aged Poll Hereford bulls, sampled at saleyards during the last 4 months of 1993, were found to be heterozygous for the mutation. In contrast, the mutant sequence was detected in only 1 of 150 (0.7%) 2- and 3-year-old Poll Hereford bulls offered for sale at 2 stud sales held during 1993, suggesting that the prevalence of the disease may decline over the next few years.     

On-column degradation and methylation of phenmedipham and desmedipham with N,N, N-trimethylanilinium hydroxide

Phenmedipham and desmedipham, when injected into a gas chromatograph with N, N, N-trimethylanilinium hydroxide, reacted to give derivatives having good gas chromatographic properties. By this procedure, these two carbamate herbicides yielded N-methylated 3-methoxyphenylcarbamates. Maximum methylation was obtained when the molar ratio of methylating reagent to herbicide was approximately 16: 1. This technique for preparing derivatives provided rapid qualitative and quantitative chromatography of the substances examined, without side-chain decomposition. The derivatives obtained were identified by means of ultraviolet, infrared and mass spectrometric procedures.     

Progressive ethmoidal haematoma in horses

Progressive ethmoidal haematoma was diagnosed in 12 Thoroughbreds, 1 part- Arab and 1 stock horse. Ages ranged from 3 to 18 years and both males and females were affected. Diagnosis was based on history, clinical signs, endoscopic and radiographic findings, and was confirmed histologically in 10 cases. Eleven (78%) of the lesions were unilateral and 3 (22%) were bilateral. Two horses were euthanased on diagnosis, 4 were treated conservatively. Of the latter, 3 were euthanased 6 to 24 months after diagnosis, due to progression of the lesions, while 1 case resolved completely within 13 months. Eight horses were treated by surgical excision of the lesion. Three died in the post-operative period, 3 were euthanased within 48 months after recurrence of the condition, and 2 had no recurrence within 60 and 72 months, respectively.