全文获取类型
收费全文 | 5283篇 |
免费 | 319篇 |
国内免费 | 4篇 |
专业分类
林业 | 269篇 |
农学 | 244篇 |
基础科学 | 9篇 |
1002篇 | |
综合类 | 610篇 |
农作物 | 575篇 |
水产渔业 | 425篇 |
畜牧兽医 | 2067篇 |
园艺 | 74篇 |
植物保护 | 331篇 |
出版年
2023年 | 28篇 |
2022年 | 70篇 |
2021年 | 116篇 |
2020年 | 97篇 |
2019年 | 107篇 |
2018年 | 137篇 |
2017年 | 160篇 |
2016年 | 185篇 |
2015年 | 165篇 |
2014年 | 200篇 |
2013年 | 285篇 |
2012年 | 350篇 |
2011年 | 419篇 |
2010年 | 223篇 |
2009年 | 221篇 |
2008年 | 314篇 |
2007年 | 301篇 |
2006年 | 269篇 |
2005年 | 248篇 |
2004年 | 232篇 |
2003年 | 189篇 |
2002年 | 203篇 |
2001年 | 144篇 |
2000年 | 120篇 |
1999年 | 77篇 |
1998年 | 33篇 |
1997年 | 18篇 |
1996年 | 20篇 |
1995年 | 21篇 |
1993年 | 21篇 |
1992年 | 28篇 |
1991年 | 28篇 |
1990年 | 33篇 |
1989年 | 37篇 |
1988年 | 31篇 |
1987年 | 40篇 |
1986年 | 18篇 |
1985年 | 32篇 |
1984年 | 23篇 |
1983年 | 27篇 |
1981年 | 19篇 |
1980年 | 15篇 |
1978年 | 21篇 |
1977年 | 19篇 |
1976年 | 28篇 |
1975年 | 14篇 |
1974年 | 20篇 |
1973年 | 22篇 |
1972年 | 22篇 |
1971年 | 15篇 |
排序方式: 共有5606条查询结果,搜索用时 312 毫秒
931.
Jeong O-Young Park Hyeon-Su Baek Man-Kee Kim Woo-Jae Lee Geon-Mi Lee Chang-Min Bombay Maurene Ancheta Melanie Banzon Lee Jeom-Ho 《Journal of Crop Science and Biotechnology》2021,24(1):1-11
Journal of Crop Science and Biotechnology - Rice has been grown as a staple food in South Korea for several millennia. However, its status has not stayed constant as a result of changes in South... 相似文献
932.
为了解2009年8月莫拉克台风的强大降雨在台湾南部地区引发严重的崩塌,造成500多人死亡的灾害情况.采用ArcGIS判释卫星影像崩塌地,统计分析降雨条件包括降雨强度、累积降雨量、降雨延时及重现期距等,对高屏溪流域在莫拉克台风期间之降雨与崩塌进行探讨.结果表明:1)莫拉克台风的降雨特性为长降雨延时(平均为3.3d)、高累积降雨量(平均为1 677 mm)及高降雨强度(平均为80.5 mm/h);2)尾寮山雨量站在48 h之累积降雨量(2319 mm)接近台湾降雨量纪录(2361 mm);3)尾寮山、御油山与溪南雨量站降雨延时在10~72 h之间,降雨强度皆大于尾寮山雨量站200 a之重现期距;4)风灾后新增崩塌面积及新增崩塌率分别为141.24 km2及4.92%,当累积降雨量达500 mm或降雨强度达55 mm/h时,崩塌地明显增加,崩塌率随累积降雨量及降雨强度的增加而有上升的趋势. 相似文献
933.
It was reported that increased plasma levels of free fatty acids (FFAs) are associated with profound insulin resistance in skeletal muscle and may also play a critical role in the insulin resistance of obesity and type 2 diabetes mellitus. Skeletal muscle is the major site for insulin-stimulated glucose uptake and is involved in energy regulation and homeostasis. In this study, we used 12-O-tetradecanoylphorbol 13-acetate (TPA), a protein kinase C (PKC) activator, and palmitate to induce insulin resistance in C2C12 mouse skeletal muscle cells. Our data show that epigallocatechin gallate (EGCG) and curcumin treatment reduce insulin receptor substrate-1 (IRS-1) Ser307 phosphorylation, and curcumin is more potent to increase Akt phosphorylation in TPA induction. Moreover, we found that after 5 h of palmitate incubation, epicatechin gallate (ECG) can suppress IRS-1 Ser307 phosphorylation and significantly promote Akt, ERK1/2, p38 MAPK, and AMP-activated protein kinase activation. With a longer incubation with palmitate, IRS-1 exhibited a dramatic depletion, and treatment with EGCG, ECG, and curcumin could reverse IRS-1 expression, Akt phosphorylation, and MAPK signaling cascade activation and improve glucose uptake in C2C12 skeletal muscle cells, especially ECG and curcumin. In addition, treatment with these polyphenols can suppress acetyl-CoA carboxylase activation, but only EGCG could inhibit lipid accumulation in the intracellular site. These findings may suggest that curcumin shows the best capacity to improve FFA-induced insulin resistance than the other two, and ECG was more effective than EGCG in attenuating insulin resistance. 相似文献
934.
Park JM Kim M Min J Lee SM Shin KS Oh SD Oh SJ Kim YH 《Journal of agricultural and food chemistry》2012,60(16):4053-4059
For the identification of a novel insecticidal protein, a two-dimensional liquid chromatography (PF-2D) system was used in a quantitative proteomic analysis of Xenorhabdus nematophila CBNU strain isolated from entomophagous nematode Steinernema carpocapsae . Protein patterns obtained from minimum and maximum insecticidal activities during cultivation were contrasted, and a novel toxin protein (Txp40) was identified by MALDI-TOF/MS. The DNA sequence of the cloned toxin gene (1089 bp) has an open reading frame encoding 363 amino acids with a predicted molecular mass of 41162 Da. The txp40 identified in this study is most closely related to the known txp40 cloned from X. nematophila EB (ADQ92844) with 94.4% identical sequence residues. Following the expression of the newly identified toxin gene in Escherichia coli , the insecticidal activity of the recombinant toxin protein was determined against Plutella xylostella larvae; a 56.7% mortality rate was observed within 24 h. 相似文献
935.
Kang MH Lee MS Choi MK Min KS Shibamoto T 《Journal of agricultural and food chemistry》2012,60(10):2517-2524
Diabetes mellitus, which is associated with oxidative damage, has a significant impact on health, quality of life, and life expectancy. An ethanol extract of Gymnema sylvestre leaf was examined in vitro and in vivo to investigate the role of antioxidants in diabetic rats. The extract exhibited strong antioxidant activity in the assays, including TBA (56%), SOD-like (92%), and ABTS (54%). Blood glucose levels in the diabetic rats fed G. sylvestre extract decreased to normal levels. The presence of the antihyperglycemic compounds gymnemagenin and gymnemic acids in G. sylvestre extract was detected by LC/MS analysis. Lipid peroxidation levels were decreased by 31.7% in serum, 9.9% in liver, and 9.1% in kidney in the diabetic rats fed the extract. Feeding G. sylvestre extract to the diabetic rats decreased the activity of glutathione peroxidase in cytosolic liver and glutamate pyruvate transaminase in serum to normal levels. 相似文献
936.
This paper investigated the feasibility of manipulating packaging polymers with various degrees of hydrophobicity to release two antioxidants, tocopherol and quercetin, at rates suitable for long-term inhibition of lipid oxidation in food. For example, one antioxidant can be released at a fast rate to provide short-term/intermediate protection, whereas the other antioxidant can be released at a slower rate to provide intermediate/long-term protection of lipid oxidation. Controlled-release packaging films containing tocopherol and quercetin were produced using ethylene vinyl alcohol (EVOH), ethylene vinyl acetate (EVA), low-density polyethylene (LDPE), and polypropylene (PP) polymers; the release of these antioxidants to 95% ethanol (a fatty food simulant) was measured using UV-vis spectrophotometry, and Fickian diffusion models with appropriate initial and boundary conditions were used to fit the data. For films containing only quercetin, the results show that the release of quercetin was much faster but lasted for a much shorter time for hydrophilic polymers (EVOH and EVA) than for hydrophobic polymers (LDPE and PP). For binary antioxidant films containing tocopherol and quercetin, the results show that tocopherol released more rapidly but for a shorter period of time than quercetin in LDPE and EVOH films, and the difference is more pronounced for LDPE films than EVOH films. The results also show the presence of tocopherol can accelerate the release of quercetin. Although none of the films produced is acceptable for long-term lipid oxidation inhibition, the study provides encouraging results suggesting that acceptable films may be produced in the future using polymer blend films. 相似文献
937.
Lee ST Davis TZ Cook D Stegelmeier BL 《Journal of agricultural and food chemistry》2012,60(19):4849-4853
White snakeroot and rayless goldenrod cause "trembles" and "milk sickness" in livestock and humans, respectively. The toxin in white snakeroot and rayless goldenrod was identified in 1927 and 1930, respectively, as tremetol. It was reported that the toxin in white snakeroot disappears as it is dried and that completely dried plants were incapable of producing trembles or milk sickness. Conversely, it has been reported that the rayless goldenrod toxin was not destroyed by drying and that the plant is toxic either fresh or dry. In this study the concentrations of tremetone, dehydrotremetone, and structurally similar compounds were determined in white snakeroot and rayless goldenrod before and after various drying conditions. Tremetone, dehydrotremetone, and structurally similar compounds in rayless goldenrod and white snakeroot are most stable upon freeze-drying, followed by air-drying, and least stable upon oven-drying (60 °C). Also demonstrated is that tremetone is stable and that dried white snakeroot and rayless goldenrod are capable of inducing toxicosis in livestock. 相似文献
938.
Black BA Lee VS Zhao YY Hu Y Curtis JM Gänzle MG 《Journal of agricultural and food chemistry》2012,60(19):4886-4894
β-Galactosidases (β-Gal) of lactic acid bacteria produce oligosaccharides from lactose when suitable acceptor carbohydrates are present. This study aimed to elucidate the structure of oligosaccharides formed by galactosylation of N-acetylglucosamine (GlcNAc) and fucose. Crude cellular extract of Lactobacillus bulgaricus and LacLM of Lactobacillus plantarum were used as sources of β-Gal activity. Disaccharides obtained by galactosylation of GlcNAc were identified as Gal-β-(1→4)-GlcNAc or Gal-β-(1→6)-GlcNAc by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and comparison with external standards. Trisaccharides were identified as Gal-β-(1→6)-Gal-β-(1→[4 or 6])-GlcNAc by LC-MS, analysis of the MS/MS spectra of selected in-source fragment ions, and their relative retention times. LC-MS analysis revealed the presence of five galactosylated fucosides, but their linkage type could not be identified, partly due to the lack of reference compounds. β-Gal of lactic acid bacteria may serve as suitable tools for the chemoenzymatic synthesis of therapeutic oligosaccharides. 相似文献
939.
Ko JA Kim YM Ryu YB Jeong HJ Park TS Park SJ Wee YJ Kim JS Kim D Lee WS 《Journal of agricultural and food chemistry》2012,60(24):6210-6216
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries. 相似文献
940.
PD Duh ZT Chen SW Lee TP Lin YT Wang WJ Yen LF Kuo HL Chu 《Journal of agricultural and food chemistry》2012,60(32):7866-7872
Antiproliferative activity and apoptosis induction of ethyl acetate of Eucalyptus citriodora resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Δψ(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent. 相似文献