全文获取类型
收费全文 | 5317篇 |
免费 | 324篇 |
国内免费 | 4篇 |
专业分类
林业 | 269篇 |
农学 | 244篇 |
基础科学 | 9篇 |
1003篇 | |
综合类 | 609篇 |
农作物 | 587篇 |
水产渔业 | 424篇 |
畜牧兽医 | 2092篇 |
园艺 | 74篇 |
植物保护 | 334篇 |
出版年
2023年 | 28篇 |
2022年 | 89篇 |
2021年 | 116篇 |
2020年 | 98篇 |
2019年 | 110篇 |
2018年 | 141篇 |
2017年 | 162篇 |
2016年 | 184篇 |
2015年 | 165篇 |
2014年 | 199篇 |
2013年 | 287篇 |
2012年 | 350篇 |
2011年 | 418篇 |
2010年 | 226篇 |
2009年 | 222篇 |
2008年 | 316篇 |
2007年 | 301篇 |
2006年 | 271篇 |
2005年 | 248篇 |
2004年 | 232篇 |
2003年 | 189篇 |
2002年 | 203篇 |
2001年 | 145篇 |
2000年 | 119篇 |
1999年 | 77篇 |
1998年 | 32篇 |
1997年 | 18篇 |
1996年 | 21篇 |
1995年 | 21篇 |
1993年 | 23篇 |
1992年 | 28篇 |
1991年 | 27篇 |
1990年 | 34篇 |
1989年 | 37篇 |
1988年 | 32篇 |
1987年 | 40篇 |
1986年 | 18篇 |
1985年 | 32篇 |
1984年 | 23篇 |
1983年 | 27篇 |
1981年 | 19篇 |
1980年 | 15篇 |
1978年 | 21篇 |
1977年 | 19篇 |
1976年 | 28篇 |
1975年 | 14篇 |
1974年 | 20篇 |
1973年 | 22篇 |
1972年 | 22篇 |
1971年 | 15篇 |
排序方式: 共有5645条查询结果,搜索用时 15 毫秒
71.
There are several strategies that can be applied in SNP discovery as for example the locus-specific amplification of target genome regions 《分子植物育种》2007,5(2):248-249
There are several strategies that can be applied in SNP discovery, as for example the locus-specific amplification of target genome regions (Primmer et al., 2002; Van et al., 2004) or simultaneous assembly of anonymous sequences which are the product of whole genome shotgun sequencing (Webber and Myers, 1997) or reduced representation shotgun sequencing (Altshuler et al., 2000). The majority of these methods are highly cost-prohibitive, however, and there is a necessity for development of new strategies that would be more suitable for small and medium-scale laboratories. 相似文献
72.
Mun Sup Yoon Jeongran Lee Chang Yung Kim Jung Hoon Kang Eun Gi Cho Hyung Jin Baek 《Euphytica》2009,165(1):69-77
Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS),
the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean
(KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within
collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for
cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate
genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per
SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection
to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from
0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation
(G
st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies
that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions
in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions
for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide
indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information
on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop
a core collection. 相似文献
73.
Kyung-Ho Ma Jae-Gyun Kwag Weiguo Zhao Anupam Dixit Gi-An Lee Haeng-Hoon Kim Ill-Min Chung Nam-Soo Kim Jae-Sun Lee Jae-Jun Ji Tae-San Kim Yong-Jin Park 《Scientia Horticulturae》2009,122(3):355-361
Here we characterized eight novel polymorphic SSR markers, developed from an enriched genomic library of garlic (Allium sativum L.). These SSRs produced a total of 64 alleles across 90 garlic accessions, with an average of 8 alleles per locus. Values for observed (HO) and expected (HE) heterozygosity ranged from 0.16 to 0.77 (mean = 0.44) and from 0.22 to 0.86 (mean = 0.65), respectively. Six loci deviated significantly (P < 0.05) from Hardy–Weinberg equilibrium (HWE). The averages of gene diversity and PIC values were 0.65 and 0.62, respectively. The mean genetic similarity coefficient was 0.4380, indicating that among garlic accessions existed wide genetic variation. Based on 64 alleles obtained by 8 SSRs, a phenogram was constructed to understand the relationships among the 90 accessions. These newly developed SSRs should prove very useful tools for genotypes identification, assessment of genetic diversity and population structure in garlic. 相似文献
74.
Eun Ju Cho Do Jin Lee Chi Do Wee Hong Lim Kim Yong Hwa Cheong Ju Sik Cho Bo Kyoon Sohn 《Scientia Horticulturae》2009,122(4):633-637
We aimed to investigate the effects of inoculating Panax ginseng C.A. Meyer seedlings with arbuscular mycorrhizal fungi (AMF) by examining the root colonization, plant nutrition uptake, growth characteristics, and soil aggregation of P. ginseng seedlings inoculated at the time of transplantation. At 16 weeks, the AMF spore density per 30 g of fresh mycorrhizosphere in seedlings inoculated with AMF (AMF+ seedlings) was 256.8 and that in seedlings not inoculated with AMF (AMF− seedlings) was 186.3, respectively. The colonization rate of AMF in the lateral roots of AMF+ seedlings was approximately 19% higher than that in the lateral roots of AMF− seedlings. The patterns of AMF colonization in ginseng roots were similar to those of the Paris-type mycorrhizal association. Plant growth characteristics, such as plant height, root length, leaf area, number of lateral roots, fresh weight of shoots and roots, and chlorophyll content, were significantly enhanced in AMF+ seedlings compared to AMF− seedlings. The macronutrient content (P, K, and Ca) and micronutrient content (Cu, Fe, and Zn) of both shoots and roots were also significantly higher in AMF+ seedlings compared to AMF− seedlings. Furthermore, glomalin content and soil aggregation were significantly enhanced in AMF inoculated areas. Our results indicate that AMF inoculation may enhance the growth of ginseng seedlings by improving the uptake of mineral nutrients and the soil structure in mycorrhizosphere. 相似文献
75.
The primary objective of this study was to evaluate the effects of foliar boron and calcium application after harvest on the quantity and activity of pollen in the ‘Housui’ and ‘Wonwhang’ pears on a subsequent year. Pollen grains of the ‘Housui’ pear were cultured on germination medium, to which had been added boric acid (0, 25, 75, 100, 200, 300, 400, and 500 mg L−1) and calcium nitrate (0, 10, 25, 50, 100, 150, 200, 250, 300, 400, and 500 mg L−1). Boric acid, which was added to the germination media, exerted a significant stimulatory effect on both pollen germination and pollen tube growth, although pollen tube growth was inhibited at higher concentrations than 300 mg L−1. Calcium nitrate addition stimulated pollen germination, except at concentration of 500 mg L−1. However, pollen tube growth was significantly inhibited with increasing concentrations of calcium nitrate. In the orchard experiment, boron and calcium were sprayed at concentrations of 0, 100, 200, 500 or 1000 mg L−1 onto leaves after harvest, respectively. Boron and calcium content in the tissues as well as pollen production and growth were determined after these treatments. The foliar application of boron mainly resulted in an increase of boron concentration in buds. It also induced an increase in the weight of the anther and pollen in the following year. On the other hand, the foliar application of calcium resulted in an increase of calcium concentration mainly in the leaves, but pollen weight was decreased at high concentration treatment in the following year. The germination rate and tube growth of collected pollen were highest in the trees which had received boron treatment at a concentration of 200 mg L−1. In contrast, the germination rate and tube growth of collected pollen were decreased by calcium application at concentrations of 500 and 1000 mg L−1 without significant increase at lower concentrations. Consequently, the accumulation of boron in the developed buds of pear trees subjected to post-harvest foliar boron application generated positive effects on both the quantity and quality of pollen in the following year. 相似文献
76.
Fruit abscission in water dropwort (Oenanthe stolonifera DC.) was investigated by measuring fruit removal force (FRF) affected by fruit ages and plant hormones. FRF decreased rapidly 30 days after anthesis (DAA). Ethylene evolution increased rapidly at 35 DAA and ABA concentration increased gradually after 20 DAA. ACC and ethephon decreased the FRF of fruit explants and the promoting effect of ACC was delayed by silver thiosulphate and cycloheximide, but not by norbornadiene and actinomycin D. ABA enhanced fruit abscission without increasing ethylene evolution. However, the observation that CoCl2 delayed abscission induced by ABA indicates possible involvement of ethylene in the ABA effect. Treatment with NAA, fenoprop and IAA had no effect on 30 DAA fruit explants, but IAA delayed abscission of 25 DAA explants. This suggests that the effect of auxins may differ with fruit age when used as control agents. 相似文献
77.
Fu-Hui Wu Shu-Chen Shen Lan-Ying Lee Shu-Hong Lee Ming-Tsar Chan Choun-Sea Lin 《Plant methods》2009,5(1):16-10
Background
Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts, a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments. 相似文献78.
ABSTRACT: Acoustic Doppler current profilers (ADCPs) have recently been used to estimate the dynamic characteristics and biomass of sound scattering layers (SSLs) or swimming speed of fish schools and to analyze SSL spatial distribution or various behavior patterns. This paper shows that it is necessary to verify mean volume backscattering strength (MVBS, dB) values acquired from each beam for quantitative analysis of the spatial distribution or the biomass estimates of such specific targets as SSL or a fish school when using an ADCP. In this study, the SSL was selected to be a homogeneous density layer over a large area and two methods were used to verify the MVBS values from each beam of the ADCP. First, a mutual comparison among four beams was conducted after calculating MVBS from the measured echo intensity. Second, the MVBS values were verified using comparison between the calculated MVBS from the 153.6 kHz ADCP and MVBS from three frequencies of a well-calibrated scientific echosounder. Moreover, the dominant scatterers (euphausiids) were collected by a framed midwater trawl. From these samples, biological data were used to identify the different frequency characteristics between two systems, using a distorted-wave Born approximation (DWBA) theoretical backscattering model in order to assess the averaged target strength and target strength TS differences for the three frequencies. 相似文献
79.
In this study, the energy budget of the Manila clam, Ruditapes philippinarum, was evaluated after one-week acclimation periods at 5, 10, 15, 20, and 25°C. Small clams (151 ± 12 mg DW) and large clams
(353 ± 16 mg DW) were fed with the microalgae, Isochrysis galbana. Filtration rate, ingestion rate, assimilation efficiency, oxygen-consumption rate, and ammonia excretion rate were measured.
Both filtration rate and ingestion rate of small and large clams were found to be related to temperature. The highest Q
10 values were measured in the range 15–20°C for both small and large clams. Assimilation efficiency of both small and large
clams was not significantly influenced by temperature, although the maximum mean values were detected at 20°C. Oxygen consumption
rate and ammonia excretion rate of small and large clams were found to be related directly to temperature over the entire
range, with a maximum being detected at 25°C. The highest Q
10 value was estimated in the range 10–15°C with regard to oxygen consumption rate, and in the range of 15–20°C with regard
to ammonia excretion rate. Scope for growth (SFG) was positive at all temperatures, achieving a maximum value at 20°C in both
small and large clams, primarily as a consequence of the enhanced ingestion rate which offset the concomitant elevation in
the metabolic rate. In this study we have estimated the thermal optimum for this species at 20°C. 相似文献
80.
An 8-week feeding experiment was conducted to investigate the effects of different dietary macronutrient level and feeding
frequency on the growth, feed utilization, and body composition of juvenile rockfish. Triplicate groups of fish (body weight
of 4.1 g) were fed the experimental diets containing either high levels of carbohydrate (HC, 35%), lipid (HL, 13%), or protein
(HP, 55%) at different feeding frequencies (twice daily, once daily, and once every 2 days). Weight gain was affected by feeding
frequency but not by dietary composition. Weight gain of fish fed the diets once every 2 days was significantly (P < 0.05) lower than that of other groups. Daily feed intake and energy intake were affected by both dietary composition and
feeding frequency. Daily feed intake of fish fed the HC diet was significantly (P < 0.05) higher than that of fish fed the HL and HP diets at the same feeding frequency. Feed efficiency and protein efficiency
ratio were affected by both dietary composition and feeding frequency and decreased with increasing feeding frequency in the
same dietary composition. Feed efficiency and protein efficiency ratio of fish fed the HC diet were significantly (P < 0.05) lower than those of fish fed the HL diet at the same feeding frequency. Whole-body lipid content of fish fed the
HL diet was significantly (P < 0.05) higher than that of fish fed the HC and HP diets at the same feeding frequency. These results indicate that an increase
of dietary lipid level compared with dietary carbohydrate level may have the advantage of a protein-sparing effect at same
feeding frequency, and a once-daily feeding regime is more effective than twice daily or one feeding every 2 days to improve
growth performance of juvenile rockfish grown from 4 to 21 g. 相似文献