个体、家系和指数选择方法实验对比

用三种不同选择方法,对赤拟谷盗(Tribolium castaneum)21日龄蛹重向上和向下两个方向进行5个世代的选择,以比较选择方法的效果。三种方法是:个体选择法(ID),家系选择法(F)和指数选择法(IX)。试验设两个重复,每个重复含有一个相同条件下的随机交配对照组(C)。对蛹重5代选择的结果为:无论上选还是下选,各方法与对照组间差异均极显著(P<0.01),且选择反应呈不对称状。在上选组中,雌、雄、及雌雄合并时所得反应排序都是IX>ID>F。在雌雄合并中,各方法间差异极显著;雌性中 ID 和 F 之间差异显著(P<0.05),其他各方法间差异极显著;雄性中 ID 和 IX 之间差异不显著,ID 与 F 间差异显著,IX 和 F 之间差异极显著。在下选组中,排序均为 IX相似文献   

肺癌腔内后装治疗方法改进的研究

对７０例肺癌患者随机分为两组，用改进麻醉及插管方法与常规方法作了对经研究，结果表明：在改进法组中，患者剧烈咳嗽，支气管痉挛和咯血等并发症明显减少，并对两组并发症的治疗作了讨论。     

Probing gene expression in live cells, one protein molecule at a time

We directly observed real-time production of single protein molecules in individual Escherichia coli cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) and a membrane-targeting peptide was expressed under a repressed condition. The membrane-localized YFP can be detected with single-molecule sensitivity. We found that the protein molecules are produced in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule, and that protein copy numbers in the bursts follow a geometric distribution. The quantitative study of low-level gene expression demonstrates the potential of single-molecule experiments in elucidating the workings of fundamental biological processes in living cells.     

中间罐尾气回收装置的运行及其改进措施

针对金陵分公司油品储运部中间罐区储存含有H2S粗汽油、粗柴油中间料挥发外泄带来的环境问题,采用抚顺石化研究院开发的“液态吸收”法对中间储罐排出的含硫、含烃尾气进行有效吸收:经冷冻的粗柴油作为贫吸收油,吸收尾气中的烃类组分,再经30％碱液重力反应器吸收含硫物质,尾气经上述处理过程后实现达标排放.通过对运行中存在的系统压力、吸收剂温度、碱液浓度等问题进行分析,提出了相关改进措施.改进后的装置运行稳定,连续尾气吸收效果较好.     

人工林落叶松髓心方材的高频真空干燥(英文)

对截面尺寸为100mm×100mm的人工林落叶松髓心方材进行系列高频真空干燥试验,分析研究不同高频真空干燥条件下干燥质量和干燥速度的变化规律,获得合理的高频真空干燥工艺基准;为了进一步减少试材表裂,干燥前对试材进行切口和过热蒸汽预处理。结果表明:沿试材长度方向的中心位置切口和过热蒸汽预处理都能有效减少试材表裂,提高干燥质量。     

不同氮肥运筹方法对免耕抛秧稻产量影响的对比试验

免耕抛秧晚稻在氮、磷、钾肥施用量相同条件下．氮肥以基肥占20％、立苗后第一次追肥占30％、分蘖肥占20％、穗前肥占30％的运筹方法较适宜免耕抛秧稻前缓中快后稳等生长发育特点的需要,可为穗大粒多打下良好基础,从而获得较高的产量。     

Technical knitting and ergonomical design of 3D seamless compression hosiery and pressure performances in vivo and in vitro

The present study was conducted from a new and innovative view to analyze pressure performances of elastic compression hosieries (ECHs) based on a technical knitting and ergonomic design and development methodology. ECHs with pressure classes I and II have been designed and developed. Multiple influencing factors and their dynamic interaction involved in the working mechanisms of ECHs were analyzed and discussed in this study. Yarn properties, material combinations, knitting structural complex, and parameters setting of machinery elements determine the fabric elasticity, stiffness, hosiery configuration, pressure magnitudes and gradient distribution. A good agreement and significant correlation on pressure performances of the developed hosieries are present from positions B1 to D when being tested on the wooden leg model by using Medical stocking tester in vitro and on the studied subjects’ legs in vivo. A reasonable pressure gradient was presented on the leg model (i.e. 70–100 % pressure at B1 and 50–80 % at C compared with the pressure exerted at ankle B), which supported the technical knitting design methodology applied in this study. However, some differences at ankle region B existed between the in vivo and in vitro values due to differences of anatomic structures that existed on the tested ankle areas. The location of testing points and body postures being undertaken by the wearers also significantly influenced the pressure profiles in vivo. More stretch deformations in hosiery fabric are produced, as assessed by static stiffness index evaluation, at the ankle when the body posture changes from standing to supine. The developed ECHs with pressure classes I and II received positive feedback on pressure and comfort perceptions on testing spot. More explorations are needed to elevate the consistency of the in vitro and in vivo pressure values at ankle region and to investigate their long-term pressure performances in practical wear through innovative knitting technical design with considerations of ergonomic factors and conducting larger scale of wear trials in future studies.     

不同条件下冬小麦F1杂种优势效应

在灌溉和雨养条件下,研究了冬小麦Ｆ１组合的平均杂种优势和平均超标优势。结果表明,小麦杂种生态型在水地是以穗粒数为主,兼顾成穗数;在旱地是以千粒重为主,兼顾成粒数。灌溉条件下小麦强优势组合最佳配置模式为均衡型×多穗型;雨养条件下以多穗×多穗或均衡型×多穗型为主。小麦杂种优势正反交差异在水地不明显,在旱地则具有较强的正反交效应。旱地小麦强优势组合亲本不仅要具有高的一般配合力,而且要具有较强优势的特异性状。     

脱涩与1-MCP处理对柿果实生理生化特性的影响

为了测试脱涩与1-MCP处理对柿果实室温贮藏品质的作用,探讨1-MCP对柿果实成熟衰老影响的生理生化机制,以‘火柿’为材料,分别进行1-MCP、脱涩和CO2+1-MCP处理,以未处理果实作为对照(CK),测定各处理果实室温贮藏期间呼吸、乙烯释放速率、果实品质、保护酶活性等的变化。结果表明:1-MCP处理推迟了CK和脱涩果实乙烯高峰的出现,延缓了果实硬度下降,有助于保持CK果实可溶性固形物和可滴定酸质量分数。1-MCP处理降低了果实过氧化物酶活性,减少了丙二醛生成,使CK果实和CO2脱涩果实室温贮藏期分别从25d和5d延长到37d和25d。1-MCP对涩柿果实商业化保鲜有较好的应用前景。     

I群禽腺病毒血清4型广东株的分离鉴定及分子特性

[目的]对广东地区疑似心包积液–肝炎综合征患病鸡进行腺病毒分离鉴定并研究其分子特征.[方法]采集的病料样品,通过鸡胚有限稀释法经SPF鸡胚传代分离纯化病毒,进行致病性试验,并对病毒的hexon、fiber-2、ORF19和ORF29基因进行PCR扩增,分析其分子特征.[结果]hexon基因序列分析显示此次分离的毒株为Ⅰ群禽腺病毒血清4型(命名为WM-XC株),SPF鸡接种分离毒株后,致死率达90%,死亡鸡只出现明显的心包积液、肝炎等变化,肝脏病理切片可观察到明显的嗜酸性包涵体,显示分离毒株具有致病性.fiber-2基因序列同经典毒株ON1和KR5相应序列的相似性达96.1%和97.0%,与2015年以来国内分离的毒株序列相似性则达到100%;ORF29序列与2013年国内分离到的毒株JSJ13和JN13的相应序列相比存在33个碱基的缺失;WM-XC分离株与2015年以来国内分离毒株序列相同,但相比经典株ON1和KR-5,缺失ORF19序列.[结论]此次从广东地区分离到的血清4型Ⅰ群腺病毒,和近年国内其他地区分离的流行毒株一样,其fiber-2、ORF19和ORF29基因序列与经典毒株差异明显.