Solubility and uptake of heavy metals from a Swedish soil

Samples of the surface layer (Ap) and of grass, collected from: (1) grass ley fertilized in the normal way; (2) permanent pasture fertilized in the normal way; and (3) permanent pasture treated with large amounts of sewage sludge five years earlier, were analysed for Mn, Zn, Cu, Cr, Co, Ni, Pb, and Cd.The soil samples were extracted with: (1) distilled water saturated with CO₂; (2) 1 M neutral ammonium acetate; (3) ammonium acetate + acetic acid, pH 4.75; and (4) 2 M nitric acid on a waterbath. The efficiency of these extractants differed greatly and, in relative values, was: 1 for H₂O + CO₂, 3.4 for NH₄OAc, 20.7 for NH₄OAc + HOAc, and 343 for 2 M HNO_3- The dissolving effects of the extractants differed markedly with the kind of element.Grass from the field treated with sewage sludge showed much higher contents of Mn and Zn and somewhat higher contents of Cu and Pb than grass from the untreated field. The levels of Cr, Co, Ni, and Cd were practically uninfluenced by the treatment. Grass from a field close to a highway accumulated large amounts of air-borne Pb and Cd during the summer.It is concluded that the total contents of heavy metals in soils have only limited importance for the uptake by plants. Weak extractants therefore give better information about the plant-available amounts in soils.     

Comparison of three enzyme-linked immunosorbent assays for serologic diagnosis of contagious agalactia in sheep.

Serologic diagnosis of ovine contagious agalactia (Mycoplasma agalactiae) with the enzyme-linked immunosorbent assay (ELISA) developed by Agence Fran?aise de Sécurité Sanitaire des Aliments (AFSSA) may produce a few false-positive (FP) and false-negative (FN) results. When the prevalence of disease is low, these erroneous results may generate problems for eradication schemes. To prevent this, 2 commercial ELISAs were compared with the AFSSA ELISA. Flocks of known status were selected and classified into 4 categories: true positive (TP), FP, true negative (TN), and FN; 20 sheep per flock were submitted for blood sampling. A flock was considered positive when at least 1 out of 20 sera was positive or 2 sera were doubtful. In the flock, the diagnostic sensitivity of the 3 kits was very good (100%), and the diagnostic specificity showed an improvement from 46% (AFSSA test) to 88% and 92% (commercial tests). Considering individual animals, very few positive ewes were detected within TN or FP flocks; the proportion of positive ewes varied greatly from one kit to another (48% to 82%) within TP flocks. The kinetics of antibody response in sheep experimentally infected with various field strains of M. agalactiae were quite similar with all 3 ELISAs. The agreement between the 3 tests, assessed using the kappa value, varied from moderate to good (respective values of 0.56, 0.61, and 0.86). The 2 commercial ELISAs showed better performances, probably because of a superior analytical sensitivity, and are a good alternative for the serodiagnosis of contagious agalactia in sheep.     

Environment and host-parasite relationships in Monogenea

The interest of a new approach in taking up the problems of host-parasite relationships with environment is proposed in terms of population biology. Two different integrating levels are considered: a microenvironment that corresponds particularly to the host and a macroenvironment corresponding to the whole host-parasite system. Some examples are given that provide significant reasons in perceiving the biology of Monogenea under these aspects. To conclude we emphasize the importance of developing this new way for the study of hybrids models in relation to parental host systems.     

Time budget and colour preferences (with specific reference to feeding) of ostrich (struthio camelus) chicks in captivity

1. Time budget analysis of the typical behaviours of young ostrich chicks showed that chicks spent over 50% of their time foraging and walking.

2. Food presented in bowls was largely ignored whereas food scattered on the floor was readily taken.

3. A colour preference test showed that green was the preferred colour.

3 Reprint requests to Dr Deeming at Hangland Farm Ostriches Ltd, Upper Wardington, Banbury, Oxon OX 17 1SU, England     

Experience with patterns of change in soil carbon resulting from forest plantation establishment in eastern Australia

Forest plantations are extensively established in eastern Australia for production of timber products and more recently, potentially for sequestration of carbon. Intensive management of these plantations involves clearing of existing vegetation, often using fire, ripping and/or mounding of the soil and weed control, followed by planting, use of fertilizers, and subsequent tending. The plantations are managed on a 10–30-year rotation and often have high growth rates and accumulation of carbon. However, after establishment, there are reduced inputs of carbon into the soil from prior vegetation or rapidly growing weeds, together with accelerated decomposition of soil organic matter as a result of disturbance, and this leads to a net loss of soil organic carbon. In some systems this loss of soil organic carbon is not balanced by carbon biomass sequestration until 5–10 years after establishment and on some sites, a reduction in soil organic carbon may remain until the end of the rotation. The patterns of accumulation and loss of carbon vary according to location, soil type and plantation management system. The effects of intensive forest establishment on soil organic carbon were evaluated in a number of studies in eastern Australia using time sequence and chronosequence studies and comparisons of plantation soils with those from adjacent undisturbed sites. There was a general pattern of reduced carbon in surface soil immediately after plantation establishment and with time this extended deeper into the soil profile. The actual quantities varied greatly depending on the soil type. The decline was primarily a result of losses of labile carbon and was greater when the previous land use had essentially been native vegetation or highly improved pastures as opposed to regrowth woodland, or native pasture, or degraded land. In the absence of further disturbance, soil organic carbon can accumulate to pre-establishment levels but many short rotation plantations are terminated prior to this being attained. The potential upper level of accumulation of soil carbon can be increased by alteration of the soil nutritional status using fertilizer application.     

Boron nutrition of Pinus radiata in relation to soil development and management

Nutritional surveys in New South Wales Pinus radiata plantations have shown differences in both foliage and soil boron status, particularly as a result of variations in soil parent materials. Plantations on acid igneous parent materials are particularly susceptible to the development of B deficiency, which appears to be further exacerbated bu soil/landscape relationships, water stress and certain management practices.

Boron deficiency in P. radiata on acid igneous soils at Sunny Corner State Forest, N.S.W., was particularly noticeable on lower slopes which usually have relatively high available moisture. These lower-slope soils had severely leached surface horizons, less extractable B, and fewer B-sorption characteristics than the upper sections of the soil catena. These soil properties also adversely affect the capacity of these soils to retain either Na-borate or Ca-borate fertilizers (borax and colemanite, respectively). For equal amounts of B, up to 95% of the borax applied to in-situ soil columns rapidly moved beyond the top 0.3 m of the soil profiles within 21 months, while less than 16% of the colemanite had moved beyond the columns. Leached surface soils of the lower-slope site retained lesser amounts of either B-fertilizer.

Colemanite offers the most cost-effective means of maintaining adequate levels of soil-B in these soil types due to its lower solubility. Management of B deficiency in P. radiata involves appropriate site selection, forest management practices and fertilizers usage.     

Site variation in Pinus radiata plantations and implications for site specific management

685,000 ha of Pinus radiata(D. Don) plantations in Australia werecategorized using a matrix of mean annualrainfall, rainfall regime and Parent Rock Codefrom a Technical Soil Classification. Datawere obtained from a number of differentorganizations which in total managed nearly allthe Australian P. radiata plantations. The selected characteristics were those whichcould be obtained at an acceptable level ofconfidence from all sources. Such acharacterization of plantation sites provided asystem for utilization of technical informationand extrapolation of research results and alsoa basis for the development of Site SpecificManagement systems. Analysis of theinformation indicated the high degree ofvariability of environments in whichplantations have been established withinAustralia and also the distinctive nature ofindividual regions. Such differences betweenareas make extension of silvicultural andrelated information difficult from one area toanother without appropriate validation. Abroad comparison with 1.26 million hectares ofP. radiata plantations in New Zealandindicated only a small overlap of areas of sitetypes between Australia and New Zealand. Assumptions derived in one location on thesites, characteristics, models and/or genotypeswould require significant testing beforeconfident application could be undertaken toanother area. The value of a Site SpecificManagement system for research planning andapplication of research and operational resultswas demonstrated from analysis of a series ofexperimental trials assessing application offertilizer after thinning and from evaluationof soil carbon in P. radiataplantations.     

Comparison of four diagnostic tests for the identification of serum antibodies in small ruminants infected with Mycoplasma agalactiae

AIM: To determine the diagnostic capability of a newly developed Western blot (WB) assay for the detection of serum antibodies against Mycoplasma agalactiae compared with conventional serological tests, and to identify the best test for routine diagnostic use. METHODS: The serological test methods used were: two commercial indirect enzyme-linked immunosorbent assays (ELISA), viz ELISA-1, using a bacterial antigen preparation, and ELISA-2, using a recombinant protein (lipoprotein p48) antigen; the complement fixation test (CFT); and a newly developed WB assay, the latter both using a bacterial antigen preparation. Thirty sera from goats infected with M. agalactiae and 97 sera from non-infected sheep were tested using all four methods. RESULTS: Staining patterns in the WB were quite variable. An immuno-dominant band of 41 kDa was detected in 63% of sera from infected animals. The same band also appeared, although mostly very weakly, in 10% of sera from non-infected animals. When suspicious or very weak reactors were omitted, the diagnostic sensitivity (DSE) and diagnostic specificity (DSP), respectively, for the four assays were: WB=56.7%, 97.9%; ELISA-1=76.7%, 99.0%; ELISA-2=56.7%, 100%; and CFT=40.0%, 94.8%. CONCLUSIONS: ELISA-1 performed best in this comparison. While the WB can be used, it did not have a technical advantage over the ELISA. The CFT should be discouraged as the primary screening method for contagious agalactia and should be replaced by ELISA-1. Results from this study confirm that serological test methods for contagious agalactia are useful for the detection of infected flocks but will not detect every individual infected animal.     

The use of tramadol in a Labrador retriever presenting with self-mutilation of the tail

A 30-month-old Labrador retriever bitch with a history of atopy was presented for acute-onset tail biting that was leading to self-mutilation. The problem began 8 months before consultation and was resolved after 2 months, but it recurred 3 months later and continued for 3 months until the time of consultation. The current episode was difficult to interrupt and was being controlled through the use of an Elizabethan collar. There had been no history of injury to the tail. On examination of the tail, an irregularity was palpated approximately midway on the dorsal surface. Radiographs of the tail showed soft-tissue swelling cranially and presence of an ossicle between the mid-caudal vertebrae. Mutilation stopped with administration of tramadol and paracetamol and started again when the medication was discontinued. No behavior modification was implemented. Although there is a possibility that the tramadol was treating a psychogenically driven self-mutilation behavior, it is more likely that pain was the initiating cause for the behavior. This case report highlights the importance of careful medical evaluation of suspected behavioral problems and discusses the possible use of tramadol in self-mutilation.