绵羊卵泡发育过程中关键信号通路研究进展

卵泡从原始卵泡发育为成熟卵泡，直至排卵、黄体发育等过程都受到精密的调控，产生大量的优势卵泡是绵羊产多羔及实现快速扩繁的关键因素。研究发现，相关信号通路和转录因子通过影响绵羊卵泡中卵母细胞、颗粒细胞的生长，进而调控卵泡的发育成熟，对这些信号通路进行深入了解，有助于探索卵泡发育的调控机制，早日实现绵羊高效繁育。Notch是卵泡发育过程中发挥重要作用的高度保守信号通路，PI3K/AKT/mTOR信号通路各成员都是广泛存在于细胞内的信号转导分子，在卵泡发育早期发挥了主要作用，还有间隙连接（gap junction，GJ）和跨带突触（transzonal projections，TZPs）等物理连接方式，在细胞间的交流通讯起到重要作用。作者详细介绍了Notch信号通路、PI3K/AKT/mTOR信号通路、间隙连接及跨带突触的结构功能在绵羊卵泡发育中的调控作用，为进一步探明绵羊卵泡发育的调控机制提供参考。     

Phylogenetic analysis of Myanmar indigenous chickens using mitochondrial D-loop sequence reveals their characteristics as a genetic resource

Myanmar indigenous chickens play important roles in food, entertainment, and farm business for the people of Myanmar. In this study, complete mitochondrial D-loop sequences (1232 bp) were analyzed using 176 chickens, including three indigenous breeds, two fighting cock populations, and three indigenous populations to elucidate genetic diversity and accomplish a phylogenetic analysis of Myanmar indigenous chickens. The average haplotype and nucleotide diversities were 0.948 ± 0.009 and 0.00814 ± 0.00024, respectively, exhibiting high genetic diversity of Myanmar indigenous chickens. Sixty-four haplotypes were classified as seven haplogroups, with the majority being haplogroup F. The breeds and populations except Inbinwa had multiple maternal haplogroups, suggesting that they experienced no recent purifying selection and bottleneck events. All breeds and populations examined shared haplogroup F. When 232 sequences belonging to haplogroup F (79 from Myanmar and 153 deposited sequences from other Asian countries/region) were analyzed together, the highest genetic diversity was observed in Myanmar indigenous chickens. Furthermore, Myanmar indigenous chickens and red junglefowls were observed in the center of the star-like median-joining network of 37 F-haplotypes, suggesting that Myanmar is one of the origins of haplogroup F. These findings revealed the unique genetic characteristic of Myanmar indigenous chickens as important genetic resources.     

睾丸支持细胞的功能、分离纯化及鉴定研究进展

支持细胞对维持精子形成过程中的微环境起决定作用,它可以通过分泌功能、细胞间连接形成的血睾屏障功能以及吞噬功能等来促进精子的形成过程,其发育异常会导致不同程度的雄性生殖缺陷。基于支持细胞在雄性动物生殖过程中的作用,体外培养高纯度支持细胞可成为研究睾丸两大核心功能-精子发生和性激素分泌功能相关调节机制重要的细胞模型。此外,体外培养睾丸支持细胞也可作为生殖毒理学等新兴热点领域的细胞模型,为评估和研究环境因素对雄性生殖的影响提供便利。因此,作者系统地归纳、总结了目前关于动物支持细胞生物功能的研究及常用的体外分离纯化、培养及鉴定方法,以期为利用动物支持细胞开展雄性生殖领域的研究提供参考。     

小麦品种贵农21,贵农22抗病性研究

采用温室苗期测定和成株期人工诱发接种鉴定方法研究了簇毛麦——硬粒小麦杂种后代贵农21、22的抗病性。苗期抗性测定表明,贵农21、22对国外15个小麦条锈菌生理小种表现为免疫至近免疫反应,仅对具有毒性基因Yr10的菌系82E16表现出中度感病,对供试的国内14个主要条锈菌菌系呈观免疫至近免疫反应。田间成株期抗性试验结果显示,贵农21、22对小麦条锈病、白粉病免疫,高抗秆锈病,中抗叶锈病。小麦品种贵农21、22可作为抗病资源和生产品种加以推广应用。     

ON THE INTERFACE BETWEEN AUTOCAD AND THE ADVANCED LANGUAGE

This paper comprehensively discusses the interface between AutoCAD and the advanced language. It presents three main ways: by means of . DXF file, SCR file and AutoLISP language. The former two have been used by some people in China. This paper presents the method with its programs for its accomplishment. As to the last one, the author has developed its application with the method for its accomplishment, the problem and the handling method for deletion of library function. AutoCAD can be used as support software for microcomputer CAD. This paper provides some references for technical personnel concerned. The programs in this paper have been all verified by IBM PC/XT, GWO520-CH. The pictures are drawn by DMP-56A.     

对两个玉米雄性不育系的细胞质分类研究

本文从普通遗传学、植物病理学和分子生物学的水平对玉米雄花不育系—元徐cms—小黄和恩二激cms—大黄进行了综合性的分类研究。大田的育性反应表明，这两个不育系的育性恢复受一对显性基因控制。对花粉染色的观察发现F1可育花粉与败育花粉基本上呈1：1分离。用小斑病T小种接种，这两个不育系不表现专化感染。通过mtDNA的分析     

中间砧对“茂谷橘橙”树体和果实品质的影响

以蕉柑、芦柑和改良橙作为茂谷橘橙的中间砧，对各中间砧组合的亲和性、树体及枝梢生长表现、产量和果实品质等进行比较研究，结果表明，3种中间砧与接穗的亲和性无显著差异，均表现为亲和力强；改良橙作中间砧的冠径，春梢、夏梢、秋梢的生长量和早期产量均显著地低于蕉柑、芦柑的。高接5年后，3种中间砧对产量的影响无显著差异。3种中间砧对茂谷橘橙果实外观品质和内部品质的影响都不明显。     

罗湾水电站引水系统过渡过程研究

为了对罗湾水电站增容前后的引水系统过渡过程和机组增容改造对引水系统建筑物的影响进行研究，应用水击计算方法中的电算法进行计算，得出了调压室最低、最高涌浪水位，蜗壳处最大压力水头和机组转速最大升高。结果表明，电站增容后引水系统完全能满足有关规范和设计的要求。     

保种群体遗传结构变化的模拟研究

本文运用电子计算机模拟的方法,分析了影响畜禽保种的主要因素,并通过不同参数组合(包括起始基因频率、群体有效含量和世代数等)的模拟试验得出三个主要结论: 1 在有性繁殖的有限群体中,原封不动地保存群体基因库中的每个基因(包括频率很低的基因)是根本不可能的。 2 在完全实行随机交配的情况下,长期保存频率较高的基因也需要相当大的群体才有可能。 3 频率较高基因的保存也不能单纯依靠群体大小,还需借助于选择。只有这样才能在小群体内长期保存频率较高的基因。