杂交相思组织培养研究初报

【目的】筛选杂交相思组织培养不同培养阶段的培养基配方,为实现杂交相思工厂化育苗提供技术参考。【方法】用杂交相思嫩茎为外植体,探究生长调节剂6-BA、NAA、IBA对其诱导出芽、继代增殖和诱导生根的效果。【结果】在不添加6-BA的情况下,添加0.2mg/LNAA时芽诱导率为60.0%,当添加0.2mg/L的6-BA后,诱导率为42.0%。当NAA为0~0.5mg/L时,随着6-BA的增加,增殖系数随之增加,6-BA为0.8mg/L时,增殖系数为4.2;当6-BA为1.0mg/L以上时,继代苗的增殖系数为1.0。以改良MS为基本培养基,在NAA1.0mg/L的条件下添加0.5、1.0mg/L的IBA生根诱导率分别为17.5%、24.0%;以改良1/2MS为基本培养基,在NAA1.0mg/L的条件下,添加0.5mg/L的IBA生根率为91.7%,当添加IBA为1.0mg/L时,生根率降到50.0%;生根苗移栽基质以黄心土与河沙或蛭石混合较好,移栽成活率在85.0%以上。【结论】改良MS＋NAA0.2mg/L培养基是较适宜芽诱导的培养基;改良MS＋6-BA0.8mg/L＋NAA0.5mg/L培养基较利于继代苗的生长;改良1/2MS＋NAA1.0mg/L＋IBA0.5mg/L培养基较改良MS培养基有利于诱导生根。     

加查县产业化潜力分析与开发途径

本文全面分析了加查县生态及自然、社会等资源要素,就生产要素的合理配置、布局及区域经济发展作了全面探讨,由此,提出了以发展核桃为主导产业的可能性和必要性,找出了一条21世纪本区域内脱贫致富奔小康必由之路,对发展加查县经济有一定的指导意义和经济价值.     

小麦抗麦红吸浆虫品种遗传多样性的表型和SSR标记分析

为更深入地揭示小麦抗麦红吸浆虫品种（系）的遗传多样性,从而为进一步选育抗虫品种提供依据,在对田间虫圃1 562份小麦品种（系）损失率鉴定的基础上,取47份年度间鉴定抗性结果较为一致的材料,利用表型和SSR标记,进行遗传多样性分析。这些抗麦红吸浆虫品种农艺性状表现出较大的差异,表型聚类在遗传距离为0.68处将供试材料分为6个类群。19对SSR标记在47份不同抗性品种中检测到104个等位基因,能够将所有品种区分开来,每对引物可以检测到3～8个等位基因,平均5.47个。47个小麦品种间遗传距离为0.40～0.95,平均为0.71。SSR标记聚类分析在遗传距离为0.74处将供试材料分为6大类群。Mental测验结果表明,表型同基因型距离矩阵间存在显著正相关（r=0.76,P〈0.05）。抗虫品种晋麦65号单独聚为一类,同其余品种具有较远的亲缘关系,可作为新的抗源用于抗虫育种,并在吸浆虫发生地块推广种植。     

Investigation of the Potential Health Benefits as Lipase Inhibitor and Antioxidant of <Emphasis Type="Italic">Leopoldia comosa</Emphasis> (L.) Parl.: Variability of Chemical Composition of Wild and Cultivated Bulbs

There is a great interest in the nutritional value of vegetables and fruits and how the habitat affects nutritive and biological properties. In vitro studies here reported were performed to evaluate the inhibitory activity of formulations from edible plant on pancreatic lipase. The aim of this study was also to evaluate the biovariability of L. comosa (L.) Parl. bulbs from Italy. The wild bulbs were compared with the same cultivated species that are commonly commercialized to identify samples with the best quality for a potential therapeutic application. Hydroalcoholic extract and polar fraction of wild bulbs showed a very important pancreatic lipase inhibitory activity, with IC₅₀ values of 0.166 ± 0.005 and 0.153 ± 0.005 mg/mL, respectively. In order to characterize the extracts, gas chromatography associated with mass spectrometry (GC/MS) analysis was performed, revealing the predominance of palmitic acid. Phenolic and flavonoid composition was also evaluated. L. comosa extract obtained from wild bulbs demonstrated both antioxidant and anti-obesity activities that might be attributed to a wide range of present phenolic compounds.     

The BRCT domain is a phospho-protein binding domain

The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain-containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage-induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.     

青海高原日光温室秋冬茬西芹种植技术

西芹与我县传统种植的本地芹相比,具有药味淡、纤维含量低、脆嫩、耐寒、适应性强、产量高等特点,而且生长旺盛、叶柄变大、营养丰实,有降压、健脑、清肠、利便的作用。     

Low salinity increases survival,body weight and development in tadpoles of the Chinese edible frog Hoplobatrachus rugulosus

Water salinity affects survival, growth and metamorphosis of anuran tadpoles. Hoplobatrachus rugulosus is considered not only as a freshwater amphibian but is also found in brackish wetlands. However, whether salinity change interferes with hatching, survival, body mass and development of H. rugulosus tadpoles is unknown. We found that salinity levels of <4‰ did not affect of survival or hatching of H. rugulosus eggs. At an early larval stage, tadpoles could tolerate up to 9‰ salinity for 96 h; however, body water content decreased when salinity was >5‰. After a 3‐week experiment, body weights of tadpoles exposed to 2‰ and 4‰ salinities were higher but that of the 6‰ group was lower compared with the 0‰ group. More than 90% of tadpoles exposed to 2‰ and 4‰ salinity showed complete metamorphosis. Salinity levels <4‰ promoted survival of tadpoles better than 0‰, whereas none of tadpoles in the 6‰ group became juvenile frogs in 50 days. Time taken to reach metamorphosis was shorter for 2‰ and 4‰ (47.22 ± 0.28 and 47.26 ± 0.33 days, respectively) than for 0‰ (49.31 ± 0.35 days). Juvenile frogs in the 2‰ group had greater body weight than the control. It could be concluded that salinity of <4‰ increased survival and body weight of H. rugulosus tadpoles, and shortened the time taken to reach metamorphosis.     

Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study

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Early life history connectivity of Antarctic silverfish (Pleuragramma antarctica) in the Ross Sea

A recent population hypothesis for Antarctic silverfish (Pleuragramma antarctica), a critical forage species, argued that interactions between life history and circulation associated with glacial trough systems drive circumpolar distributions over the continental shelf. In the Ross Sea, aggregations of eggs and larvae occur under fast ice in Terra Nova Bay, and the hypothesis predicted that dispersing larvae encounter outflow along the western side of Drygalski Trough. The outflow advects larvae towards the shelf‐break, and mixing with trough inflow facilitates return toward the inner shelf. To examine the hypothesis, we compared samples of P. antarctica collected near Coulman Island in the outflow, along Crary Bank in the inflow, and a third set taken over the rest of the Ross Sea. We ruled out misidentification using an innovative genetic validation. Silverfish larvae comprised 99.5% of the catch, and the highest population densities were found in Drygalski Trough. The results provided no evidence to reject the population hypothesis. Abundance indices, back‐calculated hatching dates, length distributions and growth were congruent with a unified early life history in the western Ross Sea, constrained by cryopelagic early stages in Terra Nova Bay. By contrast, a sample in the Bay of Whales revealed much smaller larvae, suggesting either a geographically separate population in the eastern Ross Sea, or westward connectivity with larvae spawned nearby by fish sourced from troughs upstream in the Amundsen Sea. These results illustrate how hypotheses that integrate population structure with life history can provide precise spatial predictions for subsequent testing.