Antitumor effect of chlorophyllin in vitro

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC₅₀ value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G₁ phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G₁ phase.     

Regulation of MMP-2 and TIMP-3 expression by uPA signal transduction system in human bone giant cell tumor

AIM: To study effects of urokinase-type plasminogen activator (uPA) signal transduction on expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in giant cell tumor of bone (GCT). METHODS: Expression of uPAR, MMP-2 and TIMP-3 in GCT tissue was detected by immunohistochemistry. Phosphorylation level of mitogen-activated protein kinase (p44) in uPA/uPAR signal pathway in cultured GCT cells was detected by immunoprecipitation. The expression of MMP-2 and TIMP-3 in cultured cells after treatment with uPA-ATF or anti-uPAR antibody was also detected by Western blotting. RESULTS: 1) Urokinase-type plasminogen activator receptor (uPAR) was positive on the cell membrane and in cytoplasm of some mononuclear stromal cells (MSCs) and multinucleated giant cells (MGCs); 2) MMP-2 was positive in the cytoplasm and on the cell membrane of almost all of MSCs and some of MGCs. The polar distribution of MMP-2 in the cytoplasm of MGCs was especially obvious; 3) The expression of TIMP-3 of some MSCs and MGCs in GCT was much lower than MMP-2. The positive signal also showed a prominent polarity; 4) After treatment with uPA-ATF, the phosphorylation level of p44 in GCT cultured cells was much higher than the control. Addition of anti-uPAR antibody in the cells remarkably down-regulated the phosphorylation level of p44 as compared with the control group, suggesting that uPA-ATF participates cell signal transduction and this reaction can be inhibited by anti-uPAR antibody; 5) uPA-ATF cell signal pathway up-regulated expression of MMP-2 and TIMP-3, while anti-uPAR antibody down-regulated the expression of MMP-2 and TIMP-3. CONCLUSION: These results demonstrate for the first time that uPA-ATF directly regulates the expression of MMP-2 and TIMP-3 by signal transduction pathway, and the over-expression of MMP-2 and TIMP-3 may play an important role in local osteolysis of GCT.     

Expression of DNA repair gene ERCC1 and its relationship with PAH-DNA adducts in lung cancer tissues

AIM: To investigate the expression of nucleotide excision repair gene ERCC1 and its relationship with PAH (polycyclic aromatic hydrocarbons)-DNA adducts in lung cancer tissues. METHODS: ERCC1 mRNA expression and the PAH-induced DNA adducts were detected in 150 lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The effects of some exposure factors on the expression of ERCC1 gene and the connection between ERCC1 and PAH-DNA adduct was analyzed. RESULTS: Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 1 of 40 (2.5%) normal lung tissues. Smoking may suppress the expression of ERCC1 gene. The level of PAH-DNA adduct was negatively correlated with the expression of ERCC1 gene, the Spearman coefficient was -0.648, P<0.01. CONCLUSION: ERCC1 is an important nucleotide excision repair gene and may participate in the repair of DNA damage, such as PAH-DNA adduct. Low expression of ERCC1 may play an important role in the development of human lung cancer.     

华北农牧交错带退化草地土壤种子库动态变化

对华北农牧交错带不同退化演替阶段土壤种子库的数量和物种组成及与地上植被的关系进行了初步的研究,结果表明:农牧交错带草地在刈割和放牧等干扰下,发生逆行演替,土壤种子库中植物种类及种子数量呈递减的趋势,轻度退化类型土壤种子库物种组成最高,有23种植物,种子数量最多,有4 303粒,禾本科所占比例较大,为32.3%;而极度退化草地物种组成最低,有14种植物,种子数量仅有1 156粒,禾本科只占8.4%.草地逆行演替过程中植被与土壤种子库在物种组成上存在一定差别,中度退化阶段差别最大,有18种植物未在种子库中出现,极度退化阶段差别最小,有2种未在种子库中出现.     

浅谈岩生植物及其在园林中的应用

岩生植物具有植株低矮,株形紧密;根系发达,耐干旱,耐瘠薄土壤;生长缓慢,生活期长;花色艳丽等特点.岩生植物按自然分类系统分为苔藓植物、蕨类植物、裸子植物、被子植物.按光照要求分为喜光类、耐荫类、喜阴湿类.按在园林中的应用分类,分为草本类、藤木类、灌木及矮乔类、蕨类、苔藓地衣类.论述了岩生植物在园林中的应用形式、岩生植物的选择与配置等.     

荒漠草原主导种群与植物群落地上现存量的关系

以内蒙古锡林郭勒盟苏尼特右旗短花针茅荒漠草原为对象,采用随机区组放牧试验设计,结合对比分析、线性回归分析以及结构方程模型等数据分析方法,探讨荒漠草原主要种群与植物群落地上现存量的关系及在放牧条件下的变化特点.结果表明:随放牧强度增大,短花针茅、无芒隐子草和碱韭主导种群地上现存量占植物群落地上现存量的比例下降,且主导种群...     

小麦近缘野生种抗除草剂Dalapon变异体的筛选

为了发掘小麦近缘野生种抗除草剂资源,用在培养基中添加除草剂的方法对几个小麦近缘野生种细胞系进行了变异体筛选。结果表明,从黑麦草T ove中筛选到了抗4.32 mm ol.L-1D alapon的愈伤组织,该浓度下愈伤组织存活率为0.56%;从长穗偃麦草R 123中筛选到了抗2.88 mm ol.L-1D alapon的愈伤组织,存活率为0.59%;从黑麦草N apoleon和卵穗山羊草Y 100中均筛选到了抗1.44 mm ol.L-1D alapon的愈伤组织,存活率分别为0.49%和0.56%;方穗山羊草A e42的抗性水平最低,为0.72 mm ol.L-1,愈伤组织存活率为0.33%。对存活愈伤组织进行细胞水平上的鉴定表明,其仍然保持对D alapon的抗耐性;从不同浓度D alapon下筛选到的存活愈伤组织均得到了有抗性的再生植株。在种子水平上对纤毛鹅观草进行筛选,得到了0.195%的抗性植株。     

大麦开颖性状的遗传分析Ⅰ.大麦开颖性状的遗传效应和遗传模型

为了研究大麦开颖性状的遗传规律,以5个开颖品种（系）（黄长芒大麦、沪1154、1430R、99-7122、扬0187）和1个闭颖品种（91单2）为亲本,采用6^2完全双列设计,配制杂种F1．开花期利用图象处理软件Adobe photoshop测量亲本及杂种F1的开颖角度,并对所测的数据进行方差分析．同时计算公共亲本的系列方差Vr和系列协方差Wr,以测验开颖性状的遗传模型．推断其显（隐）性基因频率分布特点。结果表明：（1）亲本间和杂种间的F值分别为237．71^＊＊和162．48^＊＊,表明开颖角度在亲本间和杂种间的差异均这到极显著水平;（2）Wr依Vr的回归方程为Wr=4,5128＋0．8968Vr,r=0＋9339^＊＊,回归系数与0的差异（t=5．23^＊＊,df=4）达到极显著水平,与1的差异（t=-0．60,df=4）不显著,即开颖性状的遗传符合加性-显性模型;回归截距与0的差异（t=0．86,df=4）也不显著,即开颖性状的遗传属完全显性类型;加性、显性和环境方差的估计数依次为45．922、20．321和1．210,说明加性遗传方差比非加性遗传方差更重要;开颖显性方向和程度随亲本和组合而有所不同,平均而言．开颖性状是减效基因表现为显性,即闭颖对开颖表现为显性;（3）15对正反交杂种F1中有3对的开颖角度存在正反交差异,表明大麦开颖性状有时还受到细胞质基因的影响。     

辽宁省水稻新品种产量及产量结构与环境效应的方差分析

以辽宁省2004～2005年中晚熟水稻区试参试品种为试材,分析了水稻品种在不同试点的产量和产量结构的差异,方差分析表明:水稻品种产量的变化主要受环境效应影响,年份×地点互作效应是影响产量的主要随机因素。每穴穗数、每穗总粒数、成粒率、千粒重都与产量有显著线性效应,每穴穗数和每穗总粒数对产量的直接作用较大,每穗总粒数通过每穴穗数对产量表现为显著负向作用。     

秸秆连续还田对苏打盐碱水稻土养分及真菌群落的影响

为探明水稻秸秆长期还田对苏打盐碱土养分状况以及土壤真菌群落结构的影响,采用盆栽试验,设置0(CK)、3.0(RS1)、7.5(RS2)、12.0(RS3)、16.5(RS4)t·hm-25个梯度秸秆还田量,于连续还田5 a后测定土壤的pH值、有机质、全氮、全磷、全钾、速效养分含量以及真菌的相对丰度、Alpha多样性和群...