Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler

This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360 one-day-old broiler chickens were randomly divided into 3 groups with 4 replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7 d,and the experiment lasted for 35 d.The chickens in the group Ⅰ(positive control group) and group Ⅱ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1% fermented herbal preparations,groups Ⅱ and Ⅲ were intraperitoneal injection of 1 mL E.coli at 35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2 and IL-6 content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ (P < 0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P < 0.05),was only 23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly (P < 0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference (P > 0.05).E.coli counts was significantly decreased after injectionin group Ⅲ (P < 0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased (P < 0.05),and inhibited the propagation of E.coli,the counts of E.coli in groups Ⅰ and Ⅲ were no significant difference (P > 0.05).At 42 d,the sIgA of the intestinal fluid in group Ⅲ were higher than that of groups Ⅰ and Ⅱ with 11.99% and 36.56%,respectively(P < 0.05).The serum IgG concentrations of group Ⅲ was higher than that of groupsⅠand Ⅱwith 14.68% and 28.15%,respectively(P < 0.05).At 42 d,the IL-2 content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P < 0.05),the IL-6 of group Ⅲ was significantly lower than group Ⅱ(P < 0.05).     

连续工作方式下掺Yb光纤放大器的增益与噪声特性研究

对连续工作方式下掺Yb光纤放大器的增益与噪声特性进行理论研究,从描述掺Yb光纤放大器的速率方程与光传输方程出发,计算了掺Yb光纤放大器的增益与噪声指数,讨论在975nm泵浦光的作用下,泵浦功率、器件长度对放大器增益和噪声指数的影响。     

氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦产量及氮素利用的影响

于2019—2021年采用再裂区设计,设置氮肥、生物炭和脲酶抑制剂3个因素,主处理设5个氮水平：0、75、150、225 kg·hm^-2和300 kg·hm^-2,副处理设2个生物炭水平：0 t·hm^-2和7.5 t·hm^-2,副副处理设2个脲酶抑制剂水平：0%和2%,共20个处理,研究氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦轮作体系作物产量和氮肥吸收利用的影响。结果表明,施用生物炭显著提高夏玉米和冬小麦产量、植株氮素吸收量、氮肥表观利用率、氮素收获指数以及夏玉米地上部生物量,较不施生物炭处理分别增加4.4%和2.9%、2.3%和3.0%、25.8%和13.5%、4.9%和6.1%、4.5%;氮肥单独配施生物炭可显著提高夏玉米和冬小麦产量、植株氮素吸收量和氮肥表观利用率,且氮肥和生物炭具有显著的交互效应。施用脲酶抑制剂显著增加夏玉米植株氮素吸收量和氮肥表观利用率,较不施脲酶抑制剂处理分别提高1.5%和3.0%;氮肥单独配施脲酶抑制剂可提高夏玉米植株氮素吸收量和氮肥表观利用率,但氮肥与脲酶抑制剂无显著...     

大兴安岭落叶松林土壤水分变化规律研究

通过2003年7-8月对大兴安岭生态定位站落叶松林内0～30cm土壤容重、根系的测定及土壤质量含水量的动态监测得出如下结论10～20cm土层和20～30cm土层土壤容重的平均值分别为0.338g/cm3、1.352g/em3,随深度的增大土壤容重增大;67.9%的根系分布在10～20cm的土层中,32.1%的根系分布在20～30cm的土层中,随深度的增大根系分布减少;从垂直分布看,降雨过后,表层0～10cm土壤质量含水量首先出现最大值且损耗最快;其次为10～20cm,20～30cm土壤质量含水量最大值出现在降雨后的第四天;降雨第四天后三层土壤质量含水量比较接近;从水平分布看,随坡度下降土壤质量含水量增大,即坡底样地土壤质量含水量要高于坡顶样地.     

鄂尔多斯高原北部沙区主要植被类型下土壤要素的空间分析

鄂尔多斯北部沙区存在着极为显著的风沙地貌,在此格局基础之上,特别是在植被与土壤的相互响应过程中,二者的相互关系逐渐密切,这种反馈作用使得该区域的植被与土壤要素形成的空间分布格局具有一定的耦合性。研究这种关系可以为该地区保护植被和合理利用土地资源提供科学依据。     

自吸泵研究现状及发展趋势

自吸泵广泛用于喷灌、消防等场合。全面总结和分析了自吸泵的发展史，归纳了自吸泵自吸时间和最大自吸高度的计算模型，总结了自吸性能影响因素研究现状，介绍了回流孔大小、位置的计算公式，以及隔舌间隙及泵内储水容积和气液分离室容积的控制；阐明了轴封的气密性、吸入管路的气密性、浸入液面下的管路深度和入口管路的容积这些外部因素对自吸性能的影响机理；指出了自吸泵进一步发展的趋势和方向，为后续研究工作提供了指导性建议。     

基于人工神经网络技术的蔬菜施肥决策研究

阐述神经网络的概念和特点，着重介绍BP神经网络并在Maflab环境下建立基于BP神经网络的施肥模型，以土壤养分（氮、磷、钾)和实际产量为模型输入，以氮肥、磷肥和钾肥的施用量为模型数输出．进行网络训练，直至获得满意的施肥模型。结合投入产出物的价格对模型功能进行扩展，按利润最大化目标进行施肥决策。     

引黄济津应急调水工程河北段输水能力计算

针对引黄济津应急调水工程河北段的输水能力进行了研究。渠段的输水能力是指渠段所能通过的最大入流量，即渠段的首端断面所能通过的最大流量。根据引黄济津应急调水工程近4年的实测数据，构建了适于干河床水流推进过程渗漏损失的改进模型、小水深情况下的糙率加大模型，并采用均匀试验优选方法对水力参数进行了反演，利用非恒定流模型对引黄济津河北段渠系输水能力进行了计算。结果表明，建立的渗漏损失改进模型、小水深情况下的糙率加大模型是合理的，反演得到的参数是精确的；由于在水流推进与涨水阶段渠床的非稳定渗漏起了较大作用，所以各渠段的输水能力在非稳定输水阶段比稳定输水阶段稍大。输水能力的计算结果可以为引黄济津未来几年的输水规划与调度提供具体的指导。