Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V_H gene segments, V_H families, J_H and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V_H, D, and J_H genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3n-2, 3n-1 and 3n, suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.     

基于LED技术的现代化图书馆建设

LED照明、LED显示和LED通信等技术的发展,极大的改善了图书馆照明、展示与宣传和大数据传输的条件,带动了现代图书馆的建设。光源布局的优化、通信系统的合理设计是图书馆有效利用LED技术的基础,LED技术的发展有力推动了图书馆新兴服务的出现。     

四倍体高粱及其后代不同生长阶段营养成分的分析

对约翰逊草、四沈甜、四6A及其杂交后代饲草的营养成分进行分析,结果表明,四倍体高粱与约翰逊草杂交种及分离世代粗蛋白在孕穗期达到最高,粗脂肪含量以抽穗期最高,单位能量值和单株生物产量以抽穗期最高。综合各营养成分含量分析认为,抽穗期有效养分含量较高,能量达到最大值,为刈割的最佳时期。四沈甜×约翰逊草杂交种粗蛋白含量在8%~10.3%,F3达到10.36%。为开展高粱与约翰逊草杂交选育优质饲料作物提供参考及依据。     

Expression Profile Analysis of Genes Involved in Brassinosteroid Biosynthesis Pathway in Cotton Fiber Development

Cotton (Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear structures derived from the epidermis of the ovule.Cotton fiber development consists of four discrete yet overlapping developmental stages: initiation,elongation,secondary wall deposition,and maturation.     

灌溉模式对华南广适型超级稻产量、品质及水分利用效率的影响

以4个早晚兼用型超级稻品种(桂农占、玉香油占、合美占和胜泰1号)为材料,研究了大田条件下灌溉模式对不同基因型水稻品种的产量、品质及水分利用效率的影响。结果表明,与常规灌溉相比,半量灌溉条件下各水稻品种的每穗总粒数显著增加,稻米的整精米率和蛋白质含量显著提高,而且灌溉用水量显著减少,水分利用率的提高也达到显著水平。     

我国现代农业机械的发展方向

现代农业机械的发展是现代农业的一个重要组成部分,在信息知识和技术、全球卫星定位技术、遥感技术、地理信息技术日益成熟的今天,农业的发展越来越走向信息化智能化.从先进国家的经验和我国农业机械的现状决定了我国现代农业机械的发展方向--信息化、智能化.     

毛竹竹筒干燥技术研究

本文研究了干燥窑中毛竹竹筒干燥时,不同因素对竹筒干燥开裂的影响。重点研究了竹筒长度、壁厚、开槽处理与不开槽处理与毛竹竹筒开裂的关系。结果表明:竹筒长度越长,开裂程度较小,干燥速度较慢,所用时间越长;竹壁越厚,竹筒越不容易开裂;开槽处理的竹筒较不做处理的竹筒更容易开裂,开槽处理干燥速度更快。分析了竹筒干燥后发生变形的原因。     

水稻超高产栽培技术规范

本标准规定了水稻超高产栽培对品种要求,适宜的稻田类型．气候条件,水源条件及本田的具体栽培技术。本标准适用贵州一季杂交中稻,中、晚熟品种适宜稻区,稻田肥力中、上等,排灌水方便,地下水位低,以地表水为灌溉水,光热充足的稻田。     

热激处理对甘蓝幼苗叶片细胞膜系统热稳定性的影响

采用二因素(热激温度、热激时间)二次回归正交旋转组合设计,对耐热性不同的2个甘蓝品种的种子进行了热激处理,然后在苗期测定了甘蓝幼叶中细胞膜的电解质渗漏量。结果表明:热激处理后不耐热品种叶片中细胞膜透性的增幅大于耐热品种,热激温度、热激时间二因素与细胞膜热稳定性之间有显著的回归关系,并且回归分析出最优热激处理组合为42℃2.3 h。