珍稀濒危植物柄扁桃的花部特征比较研究

通过对2种柄扁桃花(白花和粉花)花部特征比较研究.结果表明:2种类型的花在花冠直径、花瓣长度、柱头高度等方面存在差异,而且白花的单花花粉量,花粉/胚珠比率(P/O)少于粉花.另外,白花的花粉活力和柱头可授性显著高于粉花(P0.05).     

黄瓜新品种"吉杂迷你一号"的选育

"吉杂迷你一号"黄瓜是以欧引4-1-1-1-1为母本,以自交系S1-1-4-2为父本配制而成的华南型黄瓜一代杂种.植株生长势强,以主蔓结瓜为主,全株雌性,节成性强,结瓜数多,果实商品性状优良,短棒状,果长14～16 cm,单瓜重50～80 g,果腔小,果皮深绿色,有光泽,品质好,无刺无棱基本无果粉,果型整齐一致,品质好,肉质细脆,微甜有香气,1 hm2产量10万kg.适宜春、秋大棚和日光温室栽培.     

基于ADAMS的拖拉机参数化实用模型的初步研究

提出研究拖拉机虚拟样机模型的重要性。通过应用ADAMS软件,建立拖拉机整车的虚拟样机模型,介绍前后桥系统、转向系统、车身和传动系统模型的创建过程,仿真分析给出了拖拉机行驶速度、运动轨迹和转弯半径的变化曲线,为拖拉机设计和性能分析提供了研究平台。     

Mitochondrial DNA deletion of the brain tissue of aged rats with learning and memory deficit

AIM and METHODS: The ratio of mitochondrial DNA (mtDNA) deletion was measured to find the relationship between mtDNA deletion and aged learning and memory deficit. The aged rats were divided into two groups, aged learning and memory deficit group and aged learning and memory normal group. The ratio of mtDNA deletion was measured by dilution polymerase chain reaction. RESULTS: There are deleted mtDNA (about 4834 bp) in the cerebral cortex, hippocampus and cerebellum of both young and aged rats. The ratios of deleted mtDNA were similar in the cerebral cortex,hippocampus and cerebellum of young rats (about 0.00018%). The ratio mtDNA of aged learning and memory normal rats had increased by five-fold in the cerebral cortex and hippocampus, or one-fold in the cerebellum over young rats. The ratio of aged learning and memory dificit rats had increased by one-fold in the cerebral cortex or 0.8-fold in the hippocampus or two-fold in the cerebellum over aged learning and memory normal rats.CONCLUSIONS: There was really the increase of mtDNA in aging rat brain. And this increase was double in amount in aged learning and memory deficit rats compared to the normal learning and memory aged rats. It is suggested that the mtDNA deletions in the brain regions associated with learning and memory may be contributed to the cellular and molecular mechanism of learning and memory deicit with aged rats.     

Curcumin attenuates rat lung ischemia/reperfusion injury by interfering with autophagy

AIM To investigate the role of curcumin （CUR） in lung ischemia/reperfusion （I/R） injury （LIRI） and its relationship with autophagy. METHODS 40 SD rats were randomly divided into sham operation （sham） group, I/R group, solvent （DMSO） group, CUR group and CUR+rapamycin （CUR-Rap） group. The rats were intraperitoneally injected with normal saline, DMSO, CUR or CUR+Rap before operation. After the rat LIRI model was established, the lung tissues were taken to measure W/D, TLW, IAR, and the contents of SOD and MDA were also measured to indicate the oxidative stress level. Light and electron microscopes were used to observed the morphology and ultrastrucure of lung tissues. The expression levels of autophagy-related proteins were determined by Western blot to evaluate autophagy levels. RESULTS Compared with sham group, wet weight/dry weight （W/D）, total lung water （TLW）, injured alveoli rate（IAR） and malondialdehyde （MDA） content in all other groups were increased, superoxide dismutase （SOD） activity was decreased, the levels of autophagy were increased （P<0.05）, and lung tissue injury and cell ultrastructural damage were aggravated in CUR group. Compared with DMSO group, W/D, TLW and IAR and MDA content were decreased, SOD activity was decreased, autophagy levels were also decreased （P<0.05）, and lung tissue and cell ultrastructural damage were attenuated. Compared with CUR group, W/D, TLW, IAR and MDA content were increased, SOD activity declined, the autophagy levels were increased （P<0.05）, and damage of lung tissues and cells were more serious in CUR-Rap group. CONCLUSION Curcumin attenuates the lung I/R injury in rats, and its mechanism may be related to the reduction of oxidative stress and the inhibition of autophagy.     

不同栽培密度对杂交稻浙优18产量的影响

研究不同栽培密度对杂交稻浙优18产量的影响。结果表明,有效穗数随栽培密度增加而增加,丛有效穗、穗实粒数、结实率、千粒重随栽培密度的增加而降低。在适宜的栽培密度范围内,产量随栽培密度的增加而提高,最适栽培密度为20 cm×23 cm,每667m~2产量为569.7 kg,增产效果显著。     

政和县糯玉米新品种(系)比较试验

对引进的11个糯玉米新品种(系)进行比较试验,结果表明:京科糯2000综合性状表现好,具有产量高、抗性好等特点,适宜在政和县推广种植。总结了表现较好玉米品种的产量表现及主要农艺性状。     

Effects of noninvasive delayed limb ischemic preconditioning on prognosis of myocardial infarction

AIM: To study the effects of noninvasive delayed limb ischemia preconditioning (NDLIP) on animal cardiac function, myocardial morphology and myocardial apoptosis after myocardial infarction (MI). METHODS: Healthy SD male rats[n=45, weighing (250±10) g] were randomly divided into 3 groups:MI group:the animal model of MI was established by surgical ligation of left anterior descending artery (LAD) after 2 weeks; NDLIP group:after the success of the MI animal model, NDLIP was carried out every other day until the 4th, 6th and 8th weeks; sham group:as the negative control group, the animals were taken heart LAD threading but no ligation. All rats were fed conventionally. At the end of the 4th, 6th and 8th weeks, all rats were made ventricular intubation, and then the hemodynamic parameters were recorded. The blood samples were withdrawn from the abdominal aorta and the serum was separated via centrifugation. The serum contents of Bcl-2 and Bax were measured by ELISA. Left ventricular anterior wall was homogenized. The mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ and Ⅳ in the myocardial tissues were detected by ELISA. RESULTS: At the end of the 4th, 6th and 8th weeks, compared with MI group, left ventricular systolic pressure in NDLIP group was significantly increased, while left ventricular end-diastolic pressure in NDLIP group was significantly decreased (both P<0.05). Mitochondrial respiratory chain complexesⅠ, Ⅱ, Ⅲ and Ⅳ in NDLIP group were significantly increased (P<0.05). The serum level of Bcl-2 in NDLIP group was significantly increased and Bax level was reduced remarkably (both P<0.01). CONCLUSION: NDLIP improves the hemodynamic indexes, promotes the mitochondrial respiratory function and inhibits cell apoptosis, thus improving the prognosis of MI.     

氮钾配施对设施番茄土壤微生物群落及土壤养分和盐分的影响

为了探明不同氮钾肥用量对番茄根际土壤细菌群落及土壤养分和盐分的影响,以番茄为研究对象,设置不同氮肥和钾肥用量,通过土培盆栽模拟试验,利用高通量测序技术测定根际土壤微生物及土壤养分的变化。结果表明:1)施肥使主要的优势菌群发生明显改变,过量施肥显著降低根际土壤的细菌数量,根际土壤中肠杆菌科(Enterobacteriaceae)细菌的相对丰度明显下降,但鞘脂单胞菌科(Sphingomonadaceae)和黄单胞菌科(Xanthomonadaceae)相对丰度略有增加,细菌种群分布趋于单一化。2)过量施肥导致的土壤电导率(EC)增加、C/N下降,是土壤根际细菌群落发生改变的主要原因。3)过量施肥显著降低番茄株高、茎粗,但叶片叶绿素SPAD相对含量却呈现增加趋势,叶片光合作用受到抑制,从而产量明显下降。研究表明,施肥过量是导致设施土壤次生盐渍化的主要因素,次生盐渍化导致土壤中细菌群落发生改变,植株生长受到抑制,从而产量下降。     

新疆吐鲁番地区‘无核白’葡萄水分和矿质元素周年吸收规律研究

为探究新疆吐鲁番地区‘无核白’葡萄水分和矿质元素的周年吸收规律,以4年生‘无核白’葡萄为试材,采用密闭式营养液循环供给系统栽培,避免了水分和矿质元素的流失,并设置低、中、高浓度(0.5、1.0和1.5倍标准配方浓度)的Hoagland营养液处理。在葡萄植株的生长发育过程中持续测定营养生长(新梢长度、新梢粗度、叶片面积)和生殖生长(果粒横径、果粒纵径、单果质量、可溶性固形物、可滴定酸)指标,检测和计算了不同发育阶段葡萄植株对水分和矿质元素的吸收量,并测定葡萄叶片和果实中矿质元素的含量变化。结果表明,中浓度营养液栽培的‘无核白’葡萄长势中庸,果实品质最佳,作为吐鲁番地区葡萄水分和矿质营养吸收量的参考依据,其生理需水量为每年4 149.5m~3·hm~(-2),矿质元素每公顷的吸收量分别为N 227.9 kg,P 22.9 kg,K 325.9 kg,Ca 341.8 kg,Mg 117.8 kg,Fe 1.9 kg,Mn 460.6 g,Cu 182.3 g,Zn 198.1 g,B 728.3 g。花后47~75 d,‘无核白’葡萄植株的水分和矿质元素吸收速率最大。叶片和果实中的矿质元素含量总体呈高浓度营养液处理中浓度营养液处理低浓度营养液处理的趋势。