鲜食型超甜玉米新品种朝甜603

<正>朝甜603为早熟鲜食加工兼用型超甜玉米新品种,鲜穗籽粒排列整齐、致密、金黄色,饱满度好、甜度高、皮薄无渣适口性好;植株耐热耐湿,抗倒伏、抗病性强。朝甜603( 原名超甜603) 是以E09Z28为母本,E09Z31为父本配制而成的超甜玉米杂交种。母本E09Z28是以SHE-03与金甜甜为基础材料杂交后经多代自交系选而成,父本E09Z31是以RDE-05与金凤超甜玉米(05)为基础材料杂交后     

朗乡省级自然保护区露营旅游SWOT分析与开发对策

以黑龙江省朗乡省级自然保护区为研究对象,运用SWOT方法对其露营旅游进行分析。结果表明:黑龙江省朗乡省级自然保护区发展露营旅游存在一定的劣势与威胁,但总体上的优势与机会明显,并在此基础上提出有利于朗乡露营旅游可持续发展的对策,旨在为朗乡自然保护区露营旅游的合理开发和利用提供参考。     

南瓜新品种科栗1号的选育

科栗1号是以S31-1为母本、S4-1为父本配制而成的南瓜一代杂种。植株蔓生,长势中等;分枝较少,叶小,节间短;第1雌花节位第9节左右;嫩瓜扁圆形,瓜皮绿色覆绿色斑点和浅色纵条斑纹,老瓜皮墨绿色。嫩瓜单瓜质量0.5~1.5kg,果实横径9cm左右,纵径13cm左右,糯性好,粉质,品质佳;春季露地和秋季设施栽培嫩瓜产量1500~2000kg·(667m2)-1,早春设施栽培嫩瓜产量3000kg·(667m2)-1左右。适宜浙江省及栽培条件相似的地区推广种植。     

吴茱萸有效部位的提取纯化及含量测定

目的:对吴茱萸挥发油、吴茱萸总生物碱进行提取纯化,使其纯度达到有效部位的要求。方法:采用水蒸气蒸馏法提取挥发油,采用浓度为70%乙醇对挥发油提取后的药渣提取吴茱萸总生物碱,用中性氧化铝柱层析进行纯化,采用可见-紫外分光光度法测定含量。结果:吴茱萸碱和吴茱萸次碱分别在0.404-4.040μg/m L和0.484-5.808μg/m L范围内呈良好的线性关系,回归方程分别为A=0.193C+0.0231(r=0.999 9)和A=0.1327C-0.0012(r=0.999 8),平均回收率和RSD分别为100.3%、99.6%和1.80%、1.12%,吴茱萸挥发油、吴茱萸总生物碱含量分别为0.38%和80%以上。结论:该提取纯化方法所得样品纯度高,达到了有效部位要求。     

猪用加味玉屏风缓释微丸水提醇沉工艺研究

目的:考察猪用加味玉屏风缓释微丸的水提醇沉工艺。方法:以黄芪甲苷的含量及干膏得率作为评价指标,选用L9(34)进行正交试验。结果:猪用加味玉屏风缓释微丸最佳水提取工艺为药材分别加12倍量水,提取2次,提取时间分别为0.5 h;最佳醇沉工艺为浓缩药液相对密度1.15,加醇浓度70%,静置时间为12 h。结论:优选出的水提醇沉工艺科学、合理。     

毕节地区干草调制研究

中国南方喀斯特地区干草调制受限于诸多自然因素,客观上阻碍了该类地区草食畜牧业的发展,本研究在喀斯特典型地区贵州毕节撒拉溪示范区选取扁穗雀麦(Bromus cartharticus)、高羊茅(Festuca elata)、鸭茅(Dactylis glomerata)、菊苣(Cichorium intybus)、紫花苜蓿(Medicago sativa)、白三叶(Trifolium repens)、红三叶(T.pratense)、多年生黑麦(Lolium perenne)8种牧草进行田间自然晾晒,记录各时间段牧草的重量、空气温度和湿度,并对历年该地区气候进行浅析,重点分析近3年来4、5、6、7月份的最高温和最低温变化,通过对该地区8种主要栽培牧草的干燥过程和水分散失规律的研究,结合多年降水和气温变化规律,分析该地区调制干草的可行性,以期为喀斯特地区畜牧业的发展提供一些数据支撑。结果表明,1)4种禾本科牧草的干燥速率明显优于3种豆科牧草和菊苣;2)从外观评价角度出发,白三叶、红三叶、菊苣均不适合进行牧草晾晒;3)在选择的影响指标中,对干草晾晒的影响程度表现为晾晒时间空气湿度温度;4)综合分析各气候因子,在贵州毕节地区7月份最适合进行干草调制;5)干草调制具备可观的经济收益。     

Effect of Lysine Supplement on the Digestion and Metabolism of the Rumen in Small-tail Han Sheep

5 male Small-tail Han sheep with the body weight of 45 kg at 1.5 years old,fitted with permanent rumen fistula,to study of the effect of the supplement of the untreated commercial lysine on the rumen microbiocoenosis,rumen metabolism and whole body metabolism by 5×5 Latin square design (5 groups:control,control with low urea (equal nitrogen),low lysine,control with high urea (equal nitrogen),high lysine).The amounts of lysine-HCl supplement were 0,4.0 and 8.0 g/kg diet (as DM basis) in each group respectively,with two urea supplement control groups of equal amount of nitrogen.The results showed that by supplement of 4.0 and 8.0 g/kg of lysine-HCl diet (as DM basis) respectively,the voluntary intake of dry matter of sheep were increased by 5.5%(P>0.05) and 11.8%(P<0.05) respectively.The total amount of rumen bacteria were increased by 18.9%(P<0.01) and 23.9%(P<0.01) respectively,as the amount of rumen Coccus was increased by 21.2%(P<0.01) and 30.1%(P<0.01) respectively.The amount of rumen big Bacillus was decreased by 16.7%(P<0.05) and 33.3%(P<0.01) respectively.The total VFA of was increased by 9.6%(P<0.05) and 12.2%(P<0.01) respectively,as the butyric acid was increased by 12.8%(P>0.05) and 20.2%(P<0.01) respectively.The apparent digestibility of organic matter was increased by 6.4%(P>0.05) and 10.0%(P<0.05) respectively,the apparent digestibility of crude protein was increased by 7.0%(P>0.05) and 18.3%(P<0.01) respectively;And nitrogen retention was increased by 46.4%(P>0.05) and 110.7%(P<0.01) respectively.However,there was no effect of lysine supplement on the amounts of the rumen protozoa and fungi,and the concentration of rumen ammonia nitrogen.It was concluded that the supplement of untreated commercial lysine to adult sheep would increase the voluntary intake,digestibility,nitrogen retention the rumen microbiocoenosis and the total amount of rumen bacteria,especially the rumen Coccus of adult sheep.The effect of lysine supplement could not be replaced by urea supplement completely.     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.     

不同粗纤维水平饲粮对烟台黑猪肥育性能及胴体肉品质的影响

选择2014年春季出生的生长发育良好、胎次相近、体重35 kg左右的烟台黑猪仔猪95头为研究对象,随机分为3组,低纤维水平组31头饲喂低纤维水平饲粮,中纤维水平组34头饲喂中等纤维水平饲粮,高纤维水平组30头饲喂高纤维水平饲粮。每组3个重复,每个重复10~12头。结果表明:不同粗纤维水平饲粮对烟台黑猪35~75 kg生长期和75~95 kg肥育期的平均日增重影响显著。其中,生长期平均日增重以低纤维水平组最高,分别比中纤维水平组和高纤维水平组提高14.91%(P0.05)、23.05%(P0.01);肥育期平均日增重以中纤维水平组最高,分别比低纤维水平组、高纤维水平组提高20.96%(P0.05)、5.62%(P0.05)。生长期和全期料重比以低纤维水平组最低即饲料报酬最高,分别比中纤维水平组、高纤维水平组降低6.59%、15.51%、2.85%、6.14%。饲粮粗纤维水平对烟台黑猪胴体性能影响不显著,对肉质性状中的a值及干物质含量影响显著。a值、干物质和肌内脂肪含量都以低纤维水平组最高,分别比中纤维水平组和高纤维水平组提高9.75%(P0.05)、13.72%(P0.05)、6.09%(P0.05)、8.31%(P0.05)和110%(P0.05)、122.35%(P0.05)。不同粗纤维水平饲粮对烟台黑猪背最长肌的酪氨酸和苯丙氨酸影响显著,酪氨酸和苯丙氨酸含量都以高纤维水平组最高,分别比低纤维水平组和中纤维水平组提高10.00%(P0.05)、4.05%(P0.05)、11.90%(P0.05)、5.62%(P0.05)。不同粗纤维水平饲粮对烟台黑猪背最长肌亚油酸含量影响显著,对其他3种脂肪酸影响不显著。亚油酸含量以高纤维水平组最高,比低纤维水平组、中纤维水平组分别提高35.14%(P0.05)、4.74%(P0.05),其次是中纤维水平组较高,比低纤维水平组提高29.02%(P0.05)。综合分析,烟台黑猪适宜的饲粮粗纤维水平生长期为10%,肥育期为12%。