承德避暑山庄杜梨瘿螨发生规律研究

[目的]研究杜梨瘿螨在杜梨树上的自然发生规律,并观察评估杜梨瘿螨对其他果树、蔬菜为害的可能性。[方法]野外调查结合室内观察了解杜梨瘿螨在杜梨树上的自然发生规律 室内人工接虫饲育观察杜梨瘿螨对其他多种果树、蔬菜为害的可能性。[结果]基本摸清了杜梨瘿螨在杜梨树上的自然发生规律,杜梨瘿螨在承德一年发生2代,以成螨于杜梨芽鳞内越冬,春季4月中旬在杜梨树初展叶时,越冬螨虫开始出蛰活动 7月下旬叶面上的虫瘿数达到最高峰,8月上旬后螨虫开始蛰伏越冬。接虫饲育试验表明,杜梨瘿螨仅在桃、山楂叶片上能存活较长时间,但不能形成虫瘿,在所有的供试果树和蔬菜上均不能最终成活。[结论]杜梨瘿螨活动为害期集中在4~7月,具有生态脆弱性,属单食性害虫,只能在杜梨树上形成虫瘿,对供试的多种植物不构成危害。     

可编程控制器实验箱的研制

针对可编程控制器实践教学中存在的问题,研究制作符合实际教学特点的实验箱,从而在课程实验、课程设计、毕业设计、学生科技创新以及科学研究等方面发挥作用.可编程控制器实验箱主要进行了电源部分、输入端子、输出端子、实验面板及箱体的设计,在实际应用取得了较好的效果.     

红掌缺素症状研究

对红掌亚利桑那品种进行了缺素症的研究，分别观察并分析了植株和叶片在缺乏某一种大量元素的情况下，表现出来的典型症状。     

茄子早熟育种研究Ⅱ选择指数与早熟性选择

研究结果表明,由性状果实最大周长,果实体积,门茄显蕾期,单株产量等性状构成的选择指数较好;进一步进行遗传相关分析结果认为,应用选择指数时应注意果实体积这个性状,单株产量是一个重要的辅助性状,门茄显蕾期是一个限制性因子。应用选择指数对品种评价结果认为,早熟性较好的品种有:天津早茄、西安绿茄、二民、七叶茄、白线茄和汉中荷包。     

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)     

Destruction of Trichinella spiralis spiralis during the preparation of the "dry cured" pork products proscuitto, proscuittini and Genoa salami

Genoa salami, proscuittini and proscuitto were prepared from pork carcasses that were heavily infected experimentally with Trichinella spiralis spiralis. Genoa salami was prepared with salt concentrations of 2.0%, 2.75% and 3.3%. Proscuitto was prepared by two procedures approved by Agriculture Canada. At various times postpreparation, samples of the various cured products were taken and examined by pepsin digestion and rat bioassay for the presence of viable trichinae. Water activity and pH of the cured meat were also determined. Curing of the various products was shown to destroy the Trichinella larvae. Pepsin digestion revealed that larvae progressively became loosely coiled, uncoiled and more subject to digestion (ghost larvae) during the curing process. Rat bioassay revealed the presence of viable trichinae in the proscuitto prepared using a sodium chloride salt mixture at day 34 but not at day 48 postpreparation. All other bioassays carried out on Genoa salami between 13 and 42 days postpreparation, on proscuittini between days 27 and 69 and on proscuitto between days 34 and 69 were negative for viable trichinae. Under the conditions of this study, preparing Genoa salami with salt concentrations as low as 2% did not appear to affect the destruction of Trichinella larvae.     

果树授粉昆虫——紫壁蜂、凹唇壁蜂生物学研究

紫壁蜂、凹唇壁蜂喜在人工巢管内营巢,在果园中用芦苇管和纸管设巢可回收大量蜂种.它们均为一年一代.卵、幼虫、蛹在巢管内生长发育,成虫羽化后以滞育状态在茧内越冬.早春用0—4℃冰箱冷藏蜂茧,待果树开花前1周左右在园内释放.两种壁蜂访花范围是杏、樱桃、李、桃、梨、苹果等.壁蜂访花采蜜繁衍后代,同时提高了花朵坐果率及果品质量.两种壁蜂访花速度快、工作时间长、授粉能力强,因此成为我国北方果的优良授粉昆虫.     

原发性肾病综合征患者血、尿γ-干扰素的检测及其临床意义

目的：为进一步阐明γ—干扰素(IFN—γ)在原发性肾病综合征(PNS)发生发展中的作用及检测IFN—γ的临床诊断价值。方法：对32例PNS患者及10例正常人血清、尿液IFN—γ的水平进行检测，并结合PNS病理类型及其他临床指标进行分析。结果：系膜增生性肾炎(MesPGN)患者尿IFN—γ水平显著高于正常对照组，而非增生性肾炎组却与对照组差异无显著性；其次，尿IFN—γ水平与尿纤维蛋白(原)降解产物(FDP)浓度呈正相关；但与血肌酐(Ser)、血清肌酐清除率(Ccr)、24h尿蛋白定量(24hUPQ)无相关性。血清IFN—γ水平各组问差别无统计学意义。结论：IFN—γ参与增生型PNS免疫性肾损伤过程，并可能通过局部方式起作用，尿IFN—γ检测有可能作为区分增生性肾炎和非增生性肾炎的指标。     

In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.