慢性冷暴露对小鼠肝脏抗氧化功能的影响

本试验旨在探讨慢性冷暴露对小鼠肝脏抗氧化功能的影响。体内慢性冷暴露模型建立：试验将12只3周龄C57BL/6雄性小鼠饲养1周后随机分为2组（对照组和冷暴露组）,每组6只。冷暴露组小鼠每日随机放置在4℃环境中3 h,连续4周。冷暴露结束后将所有小鼠同时安乐死,并收集其血液和肝脏。体外冷暴露模型建立：将小鼠肝细胞系AML12细胞随机分为4组（对照组、冷暴露1 h组、冷暴露3 h组、冷暴露6 h组）,先在37℃培养箱中培养24 h,然后将冷暴露组细胞置于32℃培养箱中分别亚低温冷刺激0、12、24、36 h,冷刺激结束后将细胞刮取下来进行后续试验。用酶联免疫吸附测定（ELISA）法对小鼠血清中谷胱甘肽（GSH）和丙二醛（MDA）含量进行检测,用Western Blot检测小鼠肝细胞和肝脏组织中抗氧化蛋白硫氧还蛋白-1(Trx-1)和硫氧还蛋白互作蛋白（TXNIP）的表达情况。结果显示：与对照组相比,冷刺激36 h后AML12细胞数量显著下降（P<0.05）。与对照组相比,Trx-1的蛋白表达量在组织和细胞中都显著降低（P<0.05）,TXNIP的蛋白表达量显著增加（P<0....     

Macaca irus bone mesenchymal stem cells differentiate into neuron-like cells induced by cryptotanshinone in vitro

AIM: To determine the optimal protocol and condition in which macaca irus mesenchymal stem cells (MSCs) are induced to differentiate into neuron-like cells by cryptotanshinone in vitro. METHODS: MSCs from macaca irus bone marrow were generated in vitro and induced with cryptotanshinone. The morphological changes of MSCs were evaluated by microscope. The positive percentages of neurofilament (NF), neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) expression were measured by immunocytochemistry with ABC staining. RESULTS: The result showed that MSCs were positive for CD29, CD44, CD105, CD166, and negative for CD34, CD71, CD80 and CD86. After induced with cryptotanshinone, MSCs began to display neuronal morphologies, such as contracted multipolar cell body and formed extensive networks. The percentages of positive NSE, NF expression were 68.3%±3.5%, 70.3%±1.5%， respectively. CONCLUSION: Macaca irus MSCs could be induced to differentiate into neuron-like cells in vitro by cryptotanshinone and might be applied in cell transplantation and gene therapy in nervous system disorders.     

Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

Significance of expression of extracellular matrix metalloproteinase inducer and endothelin converting enzymes in lymphoid metastasis and prognosis in non-small-cell lung carcinoma

AIM: To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and endothelin converting enzymes (ECE) in non-small-cell lung carcinoma (NSCLC), and their relationship with lymphoid metastasis and prognosis.METHODS: Expression of EMMPRIN and ECE in 77 cases of patients with NSCLC was detected immunohistochemically.The relationship of the expression of EMMPRIN and ECE with tumor size, histological type, differentiation, lymphoid metastasis, clinical stage, and prognosis were analyzed.RESULTS: The expressive rate of EMMPRIN and ECE was 66% and 45%, respectively.The expression of EMMPRIN and ECE was associated positively with lymphoid metastasis (both P<0.01, r=0.371 and 0.467, respectively), and inversely with survival time (P<0.01 and P<0.01).No relationship was found in the expression of EMMPRIN and ECE with tumor size, histological type, and differentiation (P>0.05).The expression of EMMPRIN was associated with the expression of HGF in NSCLC (P<0.05, r=0.243).CONCLUSION: The expression of EMMPRIN and ECE is associated with lymphoid metastasis and prognosis in NSCLC.Overexpression of EMMPRIN and ECE implies infavourable prognosis in NSCLC.     

Effect of glycine on body weight and lipid metabolism in mice

AIM: To study the effect of glycine on body weight and lipid metabolism in mice. METHODS: Mice were randomly divided into four groups, control, high-fat diet group (HF), glycine group (Gly), and glycine treatment group (Gly+HF). The mice were fed with normal diet and high-fat diet for 12 days, respectively, and then were treated with glycine solution by intraperitoneal injection. Finally, the serum was collected to measure serum lipid, epididymal fat-pads, pararenal fat-pads and abdominal fat-pads were taken out to weight. Moreover, pathologic changes in liver were observed. RESULTS: The body weight of HF group was obviously lower than that in control; the body weight and adipose tissue content of Gly group were obviously lower than those in control; the body weight and adipose tissue content of Gly+HF group were obviously lower than those in HF group; the levels of TC and TG in Gly group were obviously lower than those in control; there was no statistical difference in serum lipid between Gly+HF and HF groups. In addition, marked hepatic steatosis in HF group, and slight hepatic steatosis in Gly+HF group were found. CONCLUSION: Gly can decrease the body weight and adipose tissue content and serum lipid in normal diet and high-fat diet mice, and it also can prevent hepatic steatosis.     

Correlation of Plant Morphological and Grain Quality Traits with Mineral Element Contents in Yunnan Rice

Correlations between four grain quality, 24 plant morphological traits and eight element contents of 653 accessions from Yunnan rice were analyzed. P, K, Ca and Mg contents of core collection were closely correlated to the most plant morphological and grain quality traits, and there were highly significant correlations （P 〈0.01） among some traits including P content to number of stems and tillers, K content and amylose content, Ca content and plant height, Mg content and protein content. Mn, Zn, Cu and Fe contents of core collection were closely related to a few traits, such as Fe content and gel consistency （-0.1121^＊＊）, Zn content and seed setting rate （-0.1411^＊＊）, Cu content and number of grains per panicle （-0.1398^＊＊）, Mn content and plant height （-0.2492^＊＊）.     

自制包膜复合肥的养分释放特征及其对番茄光合作用的影响

氮肥施用量大,氮素利用效率低一直是困扰我国农业生产的突出问题。如何提高氮肥利用效率、减少因过量施用氮肥而引起的环境污染问题是目前国内外肥料研究的热点问题。通过水培和盆栽试验,研究了几种自制包膜复合肥的氮释放速率及其对番茄光合作用的影响。结果表明:聚合物包膜的复合肥能明显克服未包膜复合肥破裂释放模式的缺点,使养分释放更加平缓;聚合物包膜的膜厚度对肥料的养分释放期起决定性作用,养分初期溶出率随着膜厚度的增加而降低,养分释放期相对延长;增加抑制剂可以明显降低复合肥的氮释放速率。对比盆栽试验表明:随着包膜厚度的增加,番茄叶片的净光合作用、蒸腾速率和叶绿素含量明显增加;包膜中加入抑制剂也可以提高番茄叶片的净光合作用、蒸腾速率和叶绿素含量,各处理之间呈显著水平,说明对复合肥进行包膜可以降低氮的释放速率,提高番茄叶片的光能利用效率。