细胞色素C氧化酶的纯化及动力学性质研究

采用硫酸铵分级沉淀和CytochromeC -Sepharose 4B亲和层析 ,从牛心线粒体中纯化了细胞色素C氧化酶 (CytochromeCOxidase ,简称CCO ) ,其比活性为 1 7 88s- 1 ·mg- 1 ,聚丙烯酰胺凝胶电泳为一条带 ,血红素A (HemeaA)含量达到 1 5 4nmol/mg。纯化后的CCO具有典型的CCO特征 ,还原态CCO峰值出现在 60 5nm和 443nm ,氧化态CCO峰值出现在 598nm和 42 4nm。CCO的稳态动力学参数Vm1、Km1、Vm2 、Km2 分别为 1 42s- 1 、 0 53μmol/L、 2 71s- 1 、 1 81 μmol/L。     

南美斑潜蝇及其寄生蜂消长规律

对云南省南美斑潜蝇及其主要寄生蜂潜蝇姬小蜂和潜蝇茧蜂的种群消长规律进行了研究。南美斑潜蝇在昆明关上菜区于 4、7、10月出现 3个高峰。潜蝇姬小蜂在春季蚕豆上种群数量较高 ,是控制南美斑潜蝇的主要蜂种。潜蝇茧蜂在秋季大棚内种群数量较高 ,是控制大棚南美斑潜蝇的主要蜂种。这两种寄生蜂对作物寄主有明显的选择性 ,潜蝇姬小蜂在春季的蚕豆、豌豆和芹菜上种群数量较高 ,潜蝇茧蜂在秋季的莴笋上种群数量较高     

农药残留检测技术进展

文章介绍了近年来的环境残留农药检测的方法的进展,包括样品的预处理、净化、定性及定量检测方法。同时着重介绍了超临界流体萃取、超声波萃取样等品预处理领域中的等前沿技术。     

影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件

 间歇性地给菌丝块换水可以诱导菌丝产生孢子囊,进而释放游动孢子。挑取5~8块直径为8mm于利马豆或V₈汁平板上培养4~8d的菌块,置于直径7cm的培养皿内,加入蒸馏水正好没过菌块表面,每30 min换水一次,换水4次后加入15 ml Petri培养液,25℃、黑暗条件下培养18~20 h,可诱发菌块大量产孢。     

基于EOD模式的田园综合体碳平衡初步研究

中国正在不断推进农村绿色经济转型与乡村振兴战略，田园综合体等各种以生态环境为导向的农业开发模式是乡村振兴建设的新途径，驱动农业固碳减排的同时，也带动着传统农业不断向现代农业发展的转变。以EOD（Ecology-Oriented-Development，生态环境导向的开发）模式的上海市金山区枫泾镇郊野村庄田园综合体为研究对象，在现场调研与数据统计的基础上，分析了园区内的碳输入和碳输出以及碳在园内各区域间的流动和储积；同时，根据碳源和碳汇清单和估算结果，分析了田园综合体各区的碳平衡特点。结果显示，上海市金山区郊野村庄田园综合体2021年碳平衡表现为净碳汇区，净碳汇量为152.09 tC，其中排放量为588.79 tC，碳固定量为740.88 tC。田园综合体内，作物种植区通过低碳有机种植模式成为净碳汇量最大的区域，净碳汇量185.76 tC；水产养殖区通过高效的水草多级循环利用模式净碳汇量达62.20 tC；高空间利用率的立体农业区因农肥的碳排放量大表现为碳源区，净碳汇量为-10.81 tC；生产生活区是最大的碳源区，净碳汇量达-85.05 tC，人类文旅活动是其中最大的碳排放源。分析结合该田园综合体的碳流动，提出了发展高物质循环利用率、高空间利用率、低碳有机种植模式和农业科学管理四个农业降碳增汇的方向。     

The interrelationships between serum HDL subpopulations and triacyglycerol

AIM: To Study the influence of plasma TG level on the contents of serum HDL subpopulations. METHODS: Classified by the contents of serum TG, the serum level of HDL subpopulations in 106 normal TC and 183 high TC subjects were analyzed by two-dimensional gel electrophoresis coupled with immunodection method. RESULTS: The apo-AⅠcontents of per-β1-HDL, HDL3c, HDL3b and HDL3a were higher in a certain extent in TC high subjects vs corresponding TC desirable subjects. The apo-AⅠ contents of per-β1-HDL （in borer-line high TG subgroup） and HDL3b （in very high TG subgroup） were significantly higher (P<0.05 or P<0.01), while the apo-AⅠ contents of HDL2b and HDL2a were all lower in TC high subjects vs corresponding TC normal subjects. With the increase in plasma TG levels, the apoA-Ⅰ content of pre-β1-HDL increased, and it was significantly higher (P<0.05 or P<0.01) in borderline-high TG(except TC desirable subjects), high TG and very high TG subgroups vs corresponding normal TG subgroup. Contents of HDL3b and HDL3a had the same tendency, and in TC high subjects contents of HDL3b (in very high TG subgroup) and HDL3a (in every subgroups, in which levels of TG were higher) were significantly higher (P＜0.05 and P＜0.01） than in normal TG subgroup. On contrast, the apoA-Ⅰcontents of HDL2b and HDL2a following with the increase of plasma TG levels tended to become lower. Contents of HDL2b were significantly lower (P<0.05) in high TG subgroup and very high TG subgroup vs corresponding normal TG subgroup, and in TC high subjects contents of HDL2a were significantly lower (P<0.05) in very high TG subgroups vs normal TG subgroup. CONCLUSION: Our data show the general shift toward smaller size of HDL particle size with the increase in TC and TG levels. Contents of TC and TG are very important to contents of HDL subclasses.     

水稻生物技术育种现状及其展望

综述了水稻基因工程育种与细胞工程育种的研究现状与应用。指出了水稻生物技术育种存在的问题,并展望了生物技术在未来水稻育种中的开发应用前景。     

牧草品种大拿鸭茅的特征特性及栽培要点

大拿鸭茅是四川省省级引进牧草新品种,由四川省畜牧科学研究院与百绿(天津)国际草业有限公司联合申报登记。2017年通过四川省草品种审定委员会登记,登记号：2017008。大拿鸭茅是极晚熟品系与高抗寒亲本杂交而成的四倍体晚熟品种。该品种具有适口性好、消化率高、抗锈病、分蘖多等特点,再生快,每年可刈割4～5次,干草产量为10318kg/hm²左右,表现出持久高产,理想条件下可利用6～9年。     

The Transportation and Lifting Facilities in Chongqing and the Industrialised Housing

This article introduces that there are good basic conditions and big development space for the industrialized housing development in Chongqing Municipality-the biggest municipality in the country.And it introduces the capabilities of horizontal transportation by land or sea and vertical lifting in Chongqing. And it puts forward that is more economical, reliable and safer than which has the advantages of best weight lifting capacity, service radium. Lifting height and smallest site occupation and the assemble of bigger components even the whole industrialised house.