引种珐菲亚与原产地珐菲亚的氨基酸及微量元素分析

采用原子荧光法对引种栽培9个月的药用植物珐菲亚和原产地珐菲亚的氨基酸及微量元素进行比较分析,探讨引种栽培珐菲亚与原产地珐菲亚氨基酸及微量元素的变化。结果表明,引种栽培的珐菲亚与原产地珐菲亚中的氨基酸和微量元素种类相同,含量稍有差异。     

文县杨个体生物量生长规律研究

采用标准木法对文县杨地上部分生物量进行了调查,分析了地上部分各器官生物量与株高、胸径的相关关系,结果表明:文县杨地上部分生物量各器官的比例为树干56.12%、干皮7.8%、叶9.5%、枝21.4%、枝皮5.2%,其大小顺序依次为树干＞枝＞叶＞干皮＞枝皮;地上部分质量、树干质量、树皮质量、叶质量、枝质量等与株高和胸径均呈...     

川中丘陵区城墙岩群和蓬莱镇组紫色岩上土壤性质研究

采用野外调查、取样和室内实验分析相结合的方法,研究了川中丘陵区白垩系城墙岩群(K1cg)和侏罗系蓬莱镇组(J3p)两种紫色母岩上土壤理化性质特征和差异,结果表明:两种紫色岩母质发育的土壤粘粒含量低,而砂粒含量高;土壤有机质和氮素含量低,而矿质养分含量丰富;在相同的地形部位上,J3p发育的土壤粘粒、全钾、速效钾和速效磷含量高于K1cg发育的土壤,而K1cg发育的土壤pH和土壤全磷均高于J3p发育的土壤,两种紫色岩母质形成的土壤有机质和氮素差异不明显。从丘顶到丘脚,土层逐渐增厚,土壤粘粒含量逐渐递增,砂粒含量逐渐递减;土壤有机质及各种矿质养分含量均呈递增趋势。     

普通小麦品种小偃54中α/β-醇溶蛋白编码基因的克隆与序列分析

醇溶蛋白是小麦籽粒贮藏蛋白的重要组分,其组成与含量对小麦加工品质具有重要影响。本研究建立了利用PCR从普通小麦基因组BAC文库中筛选含有α/β-醇溶蛋白基因序列BAC克隆的方法,并获得9个不同的含有α/β-醇溶蛋白基因的BAC克隆。从其中鉴定出17个α/β-醇溶蛋白基因,其编码区序列长度为852~957 bp。12个序列在编码区内存在提前终止密码子,推测为假基因。其他5个成员(Gli-Xy54-1、Gli-Xy54-2、Gli-Xy54-3、Gli-Xy54-7和Gli-Xy54-13)分别编码291、310、311、287和317个氨基酸残基,都具有α/β-醇溶蛋白一级结构的典型特征。根据推导的氨基酸序列中乳糜泻病诱发因子的分布情况及多聚谷氨酰胺重复区的长度差异,推测Gli-Xy54-7可能定位于6A染色体,Gli-Xy54-2、Gli-Xy54-3和Gli-Xy54-13可能定位于6B染色体,Gli-Xy54-1可能定位于6D染色体。基因聚类分析支持了上述推论。这是第一次从普通小麦中筛选到包含α/β-醇溶蛋白基因的BAC克隆,并从中得到目标基因全长,对进一步研究普通小麦基因组中α/β-醇溶蛋白编码基因的组成、表达与功能有较好的参考价值。     

Surface discharge characteristics and gas generation law inoil-paper insulation of transformer

Based on a surface discharge model, partial discharge experiment in the simulation transformer tank is carried out to research the development characteristics and characteristic parameters’ variation law of surface discharge in transformer oil-paper insulation, and the variation characteristic of dissolved gases in oil in the discharge process is emphatically researched. The results show that the surface discharge value increases at the beginning stage of the discharge process, and reaches its maximum after 2~3 h, then it decreases gradually. As the development of partial discharge, the absolute generation speed of CO fluctuates around a certain value with small variation. While, the absolute generation speeds of H2 and CH4 increase first and then decrease, and the variation of discharge spectrum is highly similar to the variation of oil-dissolved gas’ absolute generation speed.     

Study of the Protective Effects in PEPC Transgenic Rice

The diurnal course of chlorophyll fluorescence parameter and active oxygen metabolism of flag leaves in PEPC transgenic and untransformed rice Kitaake were studied. The results showed that the photosynthetic rate under high light intensity has been increased by 50% and photoinhibition of photosynthesis in PEPC transgenic was alleviated after the introduction of PEPC gene from maize into rice. It was demonstrated that the increment of photosynthesis in PEPC transgenic was related to the introduction of PEPC gene using specific inhibitor of PEPC. Photoinhibition of photosynthesis in different genotypes exists at noon under natural condition. PEPC transgenic rice exhibited a less decrease in Fv/Fm, a less photoinhibition and a higher efficiency of light energy conversion to chemical energy and lower thermal energy dissipation.These results provided the physiological basis on the mechanism of tolerance to photoinhibition and rice breeding with high photosynthetic efficiency.     

新鲜和盐渍F21金针菇的主要成分测定

[目的]研究新鲜和盐渍F21金针菇主要成分的含量。[方法]选取F21金针菇的新鲜和盐渍品,用凯氏定氮法、索氏脂肪提取法等方法对其蛋白质、脂肪、多糖和磷元素进行了测定。[结果]F21金针菇新鲜干品和盐渍品的蛋白质的含量分别为21.63%、15.04%,脂肪含量分别为4.70%、7.94%,多糖含量分别为33.70%、45.28%,总磷含量分别为0.2837%、0.1591%。F21金针菇新鲜干品的蛋白质和磷元素含量均高于其盐渍品,分别高出30.46%、43.91%,而脂肪和多糖的含量则低于其盐渍品,分别减少68.93%、34.36%。[结论]盐渍金针菇是金针菇生产加工的有效途径。     

玉米氮营养基因型差异与生产的关系

对玉米不同氮营养基因型根系特性、氮素吸收与利用效率的差异及营养生理基础,筛选指标进行了介绍。     

吉林农业大学水稻育种的成就、进展与前景

回顾了吉林农业大学水稻育种的发展历程,水稻育种成就及进展。提出了“十五”期间水稻优质育种和超高产育种的发展战略,使吉林农业大学水稻优质育种和超高育种向以有性杂交及“籼粳架桥”为主、现代生物技术为辅的育种新目标发展。     

Distribution of Leaf Color and Nitrogen Nutrition Diagnosis in Rice Plant

Greenness and nitrogen content of each leaf on main stem of different japonica and indica ricevarieties under different nitrogen levels were investigated. Results showed that the fourth leaf from the top ex-hibited active changes with the change of plant nitrogen status. When the plant nitrogen content was low, itscolor and nitrogen content were obviously lower than those of the three top leaves. With the increase of plantnitrogen content, the color and nitrogen content of the fourth leaf increased quickly, and the differences ofcolor and nitrogen content between the fourth leaf and the three top leaves decreased. So, the fourth leaf wasan ideal indication of plant nutrition status. In addition, color difference between the fourth and the third leaffrom the top was highly related to the plant nitrogen content regardless of the variety and development stage.Therefore, color difference between the fourth and the third leaf could be widely used for diagnosis of plantnutrition. Results also indicated that the minimized color difference between the fourth and the third leaf at the criticaleffective tillering, the emergence of the second leaf from the top, and the heading was the symbol of high yield. Plantnitrogen content of 27 g kg-1 DW for japonica rice and 25 g kg-1 DW for indica were the critical nitrogen concentrations.