节水灌溉对葡萄及葡萄酒质量的影响

 研究了不同灌溉方法对葡萄生长发育、浆果品质和葡萄酒质量的影响。结果表明, 节水灌溉显著改善了土壤结构, 增加了深层土壤的根系密度; 提高了果汁糖含量, 降低了酸含量和全盐含量, 改善了pH 值; 提高了葡萄酒的感官质量, 酒体丰满, 香气浓郁, 滋味更好; 年灌溉用水量3825 ~ 4800m3# hm^-2, 比常规灌溉节水36%。     

Preparation and characterization of anti-hBLyS monoclonal antibody by DNA immunization

AIM:To establish the monoclonal antibody against human B lymphocyte stimulator (hBLyS) by DNA immunization and analyse its characterization. METHODS:The 858 bp DNA fragment of hBLyS was cloned into pcDNA3 plasmids. The cloned insert was identified by both sequence analysis and double digestion of the recombinant plasmid with restriction enzymesXho Iand EcoR I. After the splenocytes from BALB/c mice immunized with the recombinant plasmid of pcDNA3/hBLyS were fused with myeloma cells SP2/0,the hybridoma which can produce monoclonal antibodies against hBLyS were obtained. The specificity of anti-BLyS monoclonal antibody from hybridoma was verified by ELISA, Western blot and flow cytometry. RESULTS:The recombinant mammalian cell expression vector of pcDNA3/hBLyS was constructed,the sequence of the insert gene was identified to be the sequence encoding hBLy S antigen. The culture supernatants of hybridoma 9c10 were tested to be the monoclonal antibody with specificity against hBLyS on human peripheral blood CD3⁺T cell activated by hIFN-γ by ELISA,Western blot and flow cytometry.CONCLUSION:The monoclonal antibodies against hBLyS with high activity and specificity have been established successfully, and will be an useful tool in the studies of relationship between hBLyS and human autoimmunity diseases.     

Effect of lansoprazole on gastric ulceration in rats

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.     

The genetic susceptibility of HLA-DRB1 alleles in esophageal neoplasm of Hubei Han Chinese

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO².053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN²)published in GenBank.     

新疆美洲斑潜蝇寄主种类及适合度的调查

通过乌鲁木齐、昌吉等地调查，发现新疆美洲斑潜蝇的寄主植物计有17科55种，其中蔬菜28种(变种)、大田作物8种，花卉和草坪植物13种、野生植物6种。以豆科、葫芦科植物受害最重。应用欧氏距离法的系统聚类分析，研究了美洲斑潜蝇对14种常见寄主植物的适合度；将其划分为5个类型，即最优适宜寄主、良好适宜寄主、适宜寄主、稍次适宜寄主和非适宜寄主。为害虫的防治决策提供了科学依据。     

粉红女士苹果品质特性分析评价

观察测定了粉红女士苹果成熟采收时和常温贮藏后果实的外观、抗性、果实硬度、总酸、可溶性固形物、维生素C、蒸腾失水率、出汁率、果汁密度以及果汁性状;全面分析评价了该品种的外在、内在、贮藏品质和果汁加工特性。结果表明,粉红女士苹果是一个综合性状优良、耐贮藏的鲜食、果汁加工兼用型苹果新品种。     

疫霉菌对霜脲氰抗性遗传及对霜脲氰和甲霜灵的交互抗性

就疫霉菌对霜脲氰抗性的产生和遗传，以及疫霉菌对霜脲氰和甲霜灵的交互抗性进行了研究。结果表明，苎麻疫霉容易对霜脲氰产生抗药性突变，但抗性在连续３代单游动孢子分离过程中逐渐丧失。大雄疫霉对霜脲氰不易产生抗性突变，恶疫霉、大雄疫霉和苎麻疫霉对甲霜灵和霜脲氰不存在交互抗性     

粳稻品种抗稻瘟病性与其生物学性状的相关关系研究

对３４２份粳稻材料进行抗稻瘟病性（抗叶瘟、穗颈瘟）与其生物学性状（株高、穗长、每穗粒数、分蘖率、单株有效分蘖数、空秕率、着粒密度，以及成熟期等）相关关系研究，结果不同品种（系）间存在着一定差异。抗叶瘟性与株高、穗长、每穗粒数、单株有效分蘖数、着粒密度呈显著正相关，相关系数依次为：０．４０９、０．４９１、０．４２６、０．６５７、０．４７１；与空秕率呈显著负相关，相关系数为－０．５９１。抗穗颈瘟性与株高、每穗粒数、单株有效分蘖数呈显著正相关，相关系数分别为：０．７１７、０．４６２、０．３６２；与空秕率、分蘖率、着粒密度呈显著负相关，相关系数依次为：－０．３４３、－０．３３２、－０．４７６。试验表明，株高、单株有效分蘖率、每穗粒数、空秕率等性状，可做为主要标记，在粳稻品种（系）抗稻瘟病性选鉴上应用     

温度对稻茎毛眼水蝇实验种群增长的影响

试验结果表明:在17～31℃恒温范围内,稻茎毛眼水蝇的发育速率随温度上升呈逻辑斯蒂曲线趋势。幼虫、蛹存活率、成虫产卵量、种群增长指数随温度的上升呈抛物线趋势。卵孵化率在20～31℃范围内无显著差异,均在92％以上。在17℃和35℃时,孵化率下降至86.67％和76.36％。在35℃时,幼虫不能存活,成虫不产卵。     

大米粉的猪回肠氨基酸真消化率测定

大米加工副产品大米粉长期以来被添加到米糠中利用，显著降低了其利用率。本试验用三头施以回直肠吻合术猪，采用单因子设计，以大米粉为半纯合日粮，无氮日粮由醋酸纤维素提供粗纤维来测定内源值，测定了大米粉的猪回肠末端氨基酸表观消化率和真消化率。大米粉的回肠末端氨基酸的表观消化率的平均值为76．20％，真消化率的平均值为85.86％。这些数据可为我国氨基酸消化率数据库提供参考，并且可作为以可消化氨基酸为基础配制日粮时的依据。