Cardiac L-type calcium channel and its alterations in cardiac hypertrophy and heart failure

There are two type of cardiac calcium channel current, both are inward current: (1) L-type Ca²⁺channel current (I_ca-L), plays a major role in determining myocyte action potential plateau characteristics as well as initiating the myocyte excitation-contraction coupling; (2) T-type Ca²⁺ channel current (I_ca-T), probably plays an important role in pacemaker activity. The alterations of L-type calcium channel abundance and function in cardiac hypertrophy and heart failure are determined by the the species difference, especially by the stage of the disease process and the degree of the disease. Both abundance and function of L-type calcium channel decrease in severe hypertrophy and heart failure.     

Adhesion of bone marrow stromal cell in children with acute lymphoblastic leukemia

AIM and METHOD: The adhesion behavior of bone marrow stromal cells(BMSC) in children with acute lymphoblastic leukemia(ALL) to bone marrow mononuclear cells(BMMC) and Raji cells were investigated by MTT method. The expression of adhesion molecules ICAM-1 and VCAM-1 were detected by flow cytometry. RESULTS: The adhesion ability of BMSC in acute period of ALL to BMMC was lower than that of control group. The adhesion ability of BMSC in long term remission period of ALL to Raji cells higher than that of control group. The expression of ICAM-1 on the surface of BMSC in acute period of ALL is much lower than that of control group. CONCLUSIONS: The adhesion of BMSC to BMMC or tumor cells in children with ALL was abnormal. The abnormal adhesion behavior might be partially due to the changed expression of adhesion molecules on BMSC.     

Protective effect of C1q/tumor necrosis factor-related protein 3 on vascular endothelium in rats with hyperuricemia

AIM To study whether C1q/tumor necrosis factor （TNF）-related protein 3 （CTRP3）protect vascular endothelium in rats with hyperuricemia and its potential mechanisms. METHODS An animal model of hyperuricemia was established by using male SD rats drinking 10% fructose water （n=10）. The rats drinking normal water served as normal controls （n=10）. After 12 weeks, the rats were given a single injection with Ad-CTRP3 or Ad-GFP. The experiment was ended at 14th day after transfection.The serum levels of uric acid and nitric oxide （NO） were evaluated. The serum contents of TNF-α and interleukin-6 （IL-6） were measured by ELISA. HE staining and TUNEL assay were used to assess the morphological changes of intima and apoptosis of endothelial cells in thoracic aorta, respectively. The mRNA levels of endothelial nitric oxide synthase （eNOS）, TNF-α and IL-6 were detected by RT-qPCR. The protein levels of CTRP3 and Toll-like receptor 4 （TLR4） were determined by Western blot. RESULTS Compared with normal control group, the rats with hyperuricemia showed lower CTRP3 and higher TLR4 protein levels in the thoracic aorta （P<0.05）. Hyperuricemic rats had higher serum contents of uric acid, TNF-α and IL-6 （P<0.05）. Also, the intima structure disturbance of thoracic aorta, increased apoptotic rate, higher mRNA levels of TNF-α and IL-6 as well as lower mRNA levels of eNOS were observed （P<0.05）. By contrast, CTRP3 over-expression decreased TLR4 protein levels, reduced inflammatory cytokines, and obviously improved the morphology and function of thoracic aorta in the rats with hyperuricemia. CONCLUSION CTRP3 protect vascular endothelium in rats with hyperuricemia maybe via down-regulation of TLR4- mediated inflammatory signaling pathway.     

盐度变化对军曹鱼稚鱼相关免疫因子及其生长的影响

研究了盐度(5、10、20、30及对照37)对军曹鱼(Rachycentron canadum)稚鱼生长及其血清溶菌酶、碱性磷酸酶(ALP)、旁路途径补体(ACP)溶血活性(ACH50)和总免疫球蛋白(T-Ig)含量等免疫因子的影响。实验周期为14 d。结果表明,在逐渐达到设定盐度后养殖14 d,盐度30组稚鱼特定生长率(SGR)最高(5.77%/d),而盐度5和10组的SGR(分别为4.24%/d和4.38%/d)显著低于其他组(P<0.05)。在低盐度环境中血清溶菌酶活性在第7天各组都有不同程度升高,其中以盐度20组升高最为显著,其次为盐度10和30组,但第14天各组溶菌酶活性又都回落至对照组水平;而稚鱼在低盐度环境中血清ALP活性受到明显抑制,且活性与盐度呈一定正相关;各组间ACP活性在第7天各盐度组间无明显差异,但实验结束时盐度5和10两组出现显著升高;而T-Ig含量在盐度10组中始终明显高于其他组。研究显示,军曹鱼稚鱼在盐度20~37范围内都可正常生长,尽管稚鱼有较强的低盐度耐受能力,但过低盐度明显影响其生长率并导致体色变黑、蛀鳍和应激增强等异常表现。此外,盐度变化还影响稚鱼多种免疫相关因子。[中国水产科学,2007,14(1):120-125]     

两种温度条件下杀虫单对赤子爱胜蚓蛋白含量SOD和AChE活性的影响

为评价土壤的农药污染程度,选用杀虫剂杀虫单对赤子爱胜蚓(Eiseniafoetida)进行染毒试验,采用滤纸法研究两种温度下农药污染对赤子爱胜蚓的毒理和其体内蛋白含量、超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)酶活力的影响。结果发现,杀虫单单一作用时,在10℃和20℃两种温度下,蚯蚓死亡率分别为20%和30%;随杀虫单浓度的增大,蚯蚓体内蛋白含量出现不同程度的升高;SOD活性随农药浓度的升高整体表现为下降趋势,其降低率分别为32.2%和21.2%,但规律不很明显;时间和浓度对SOD的影响达到极显著(P<0.01);农药对蚯蚓体内AChE活力的抑制作用不明显,这与该农药是较弱的胆碱酯酶抑制剂有关,且随暴露时间的延长,AChE活力表现出一定的恢复现象。试验结果表明,温度对蚯蚓死亡率及蛋白含量、SOD、AChE酶的活性有一定影响,可通过对比两种温度下的蚯蚓体内酶活性的大小来进行生物监测和环境影响评价。     

公安院校图书馆队伍现状分析与对策研究

近年来,人们的思想观念和思维方式随着改革开放和社会主义市场经济体制的建立和社会生活的变化,也发生了巨大变化。我国公安院校图书馆工作人员也存在不愿意从事图书馆工作而愿意从事教学工作或公安机关其它业务部门工作的倾向,本文对产生这种现象的客观和主观原因进行了分析,并提出了解决的办法。     

中草药对奶牛常见病原菌的体外抑菌试验

采用琼脂扩散法(打孔法),选择白头翁、夏枯草、黄柏、乌梅、鱼腥草等16种中草药煎剂和复方制剂对从病牛分离出的4种主要致病菌进行了体外抑菌试验,结果发现,乌梅、黄连、白头翁、诃子、黄芩等的综合效果好,8个复方制剂除复方2和复方3对大肠杆菌无抑菌作用,其余均敏感.为中草药防治奶牛常见病的科学配方筛选提供了依据.     

土壤酸度对茶叶产量及品质成分含量的影响

为了分析土壤酸度对茶叶产量及品质成分的影响,本研究选择4个不同酸度土壤的茶园(pH分别为3.29、4.74、5.32、6.38)为研究点,于2016年4月种植铁观音茶树,于2018年5月、2019年5月(春茶采摘时间)和2018年10月、2019年10月(秋茶采摘时间)采样测定茶叶鲜叶产量及鲜叶品质成分(茶多酚、茶氨酸...     

间作对咖啡和澳洲坚果根系形态、分布及养分累积的影响

为探明澳洲坚果与咖啡间作对地上部生长及地下部互作效应,为生产推广复合高效栽培模式提供理论依据,布置了咖啡和澳洲坚果间作盆栽实验,以二者分别单作为对照,分析植株的生长量、生物量、养分含量、根系形态以及土壤养分的差异.结果表明:(1)间作咖啡和间作澳洲坚果根干重、茎干重、单株总干重分别为40.86、50.38、148.34...     

香蕉TGA转录因子家族的鉴定及枯萎病菌胁迫下的表达分析

尖孢镰刀菌（Fusarium oxysporum f. sp. Cubense, Foc）引起的香蕉枯萎病是我国香蕉的主要病害之一,严重影响香蕉的产量和品质。TGA防御反应基因在香蕉应对尖孢镰刀菌胁迫的应答转录调控过程中至关重要。本研究以拟南芥TGA转录因子家族成员蛋白为查询序列,在香蕉基因组数据库中Blast筛选出TGA转录因子家族成员,并对该家族成员进行生物信息学分析。共鉴定得到9个香蕉TGA家族成员,分别命名为MaTGA1~MaTGA9;香蕉TGA家族蛋白富含酸性氨基酸,大部分蛋白以α螺旋为主;亚细胞定位主要在细胞核内。进化树分析表明,香蕉MaTGA转录因子家族的9个成员可分为Class Ⅰ和Class Ⅱ两类,基因结构及功能结构域的分布情况也呈现出高度一致。进一步通过RT-qPCR分析发现,MaTGA2、MaTGA3和MaTGA8在香蕉枯萎病菌侵染后的‘威廉斯’（易感病）及‘南天黄’（抗病）中均显著下调表达,MaTGA1、MaTGA6、MaTGA7和MaTGA9均呈现先下降后上升的趋势;然而MaTGA4和MaTGA5仅在‘威廉斯’中上调表达,以上结果表明MaTGA2、MaTGA3和MaTGA8在香蕉抗枯萎病中发挥重要的生物学功能。本研究结果为香蕉TGA转录因子功能挖掘奠定理论基础。