文化创意产业与旅游业的融合模式研究--以五大连池风景区为例

文章从文化创意产业与旅游业的融合角度着手,以五大连池风景区为例,通过分析五大连池风景区火山与矿泉文化资源,进一步从文化创意与旅游融合的角度提出拓展型融合、渗入型融合、重装型融合的五大连池火山矿泉疗养文化与旅游业的融合模式。     

一株新疆灵芝的鉴定及生长和产糖性能初探

以新疆阿勒泰采集的灵芝子实体XJ-001为试材,采用组织分离纯化获得该菌纯培养,以菌丝体基因组DNA为模板进行ITS片段扩增,测序并进行系统发育分析;以5种不同培养基培养菌株,通过比较生长速度,选出适宜母种培养基;摇瓶发酵研究不同碳氮源对菌株菌丝体生物量、胞内及胞外多糖含量的影响。结果表明:根据形态特征结合rDNA-ITS分析,初步将XJ-001鉴定为Ganoderma lucidum,并获得GenBank登录号为KT997431;菌株在玉米粉、豆饼粉琼脂培养基长势最优,生长速度为18.7mm·d~(-1);以葡萄糖、酵母浸粉为最适碳、氮源,在转速150r·min~(-1),30℃培养5d,菌株XJ-001菌丝体生物量、胞内/外多糖含量分别为8.15、1.18、0.05mg·mL~(-1)。该菌株具有生长迅速和产糖较高的潜在性能,可为开发新疆野生灵芝资源提供参考。     

不同行道树降温增湿及滞尘效应

以海南热带海洋学院(三亚校区)校园行道树优势树种高山榕、大叶相思、印度胶树、菩提树、非洲楝、火焰木、马占相思、香樟、鸭脚木、小叶榄仁、垂叶榕、隆缘桉为研究对象,比较了乔木绿化树种的降温增湿和滞尘能力。结果表明:在相同环境生长的种龄相当的树种,降温增湿效应不同,高山榕、大叶相思、印度胶树的降温增湿能力最好;降温增湿效应比较差的是隆缘桉、小叶榄仁和垂叶榕。不同树种的滞尘能力也有差异,其中滞尘能力较强的乔木树种是火焰木、菩提树、高山榕和印度胶树。该研究结果对校园绿化和城市行道树的规划选择都具有一定的参考价值。     

不同驱虫药驱虫后伊犁马血液生化指标变化的研究

[目的] 研究不同驱虫药对伊犁马血液生化指标的影响,为伊犁马科学驱虫及健康养殖提供参考。[方法] 将平均体重（265.5±35.6）kg、出生日期相近的40匹1岁伊犁马随机分为5组,每组8匹,分别为对照组、试验Ⅰ组、试验Ⅱ组、试验Ⅲ组和试验Ⅳ组。在相同的饲养管理和日粮营养水平条件下,对照组不驱虫,在试验开始第1天,试验Ⅰ组使用伊维菌素驱虫,试验Ⅱ组使用吡喹酮驱虫,试验Ⅲ组使用阿苯达唑驱虫,试验Ⅳ组使用伊维菌素和阿苯达唑的混合药剂驱虫;在试验第14天采集马匹血液样品,测定血浆氮代谢指标、葡萄糖及酶相关指标、脂代谢指标及金属离子指标。[结果] ①试验Ⅲ组总蛋白（TP）浓度最低,与对照组、试验Ⅰ组、试验Ⅱ组及试验Ⅳ组相比,分别降低了8.16%、7.15%、4.98%及6.62%（P>0.05）。对照组及各试验组白蛋白（ALB）、尿素（UREA）浓度均无显著（P>0.05）差异。与对照组相比,试验Ⅲ组及试验Ⅳ组血浆中UREA浓度分别降低了33.15%及36.10%（P>0.05）。②与对照组相比,试验Ⅰ组、试验Ⅱ组、试验Ⅲ组、试验Ⅳ组丙氨酸氨基转移酶（ALT）活力分别降低了26.51%、21.11%、47.14%及52.38%（P>0.05）。试验Ⅲ组血浆中碱性磷酸酶（ALP）活力低于对照组,降低了0.19%（P>0.05）。与对照组相比,试验Ⅱ组和试验Ⅳ组的乳酸脱氢酶（LDH）活力显著（P<0.05）升高,分别提高了17.84%、13.51%;谷草转氨酶（AST）活力分别提高了5.40%和4.08%（P>0.05）。③对照组血浆中甘油三酯（TG）、肌酐（CREA）及尿酸（UA）的含量与各试验组相比均无显著（P>0.05）差异。试验Ⅰ组及试验Ⅳ组血浆中CREA浓度较对照组分别提高13.38%和9.86%（P>0.05）。试验Ⅱ组血浆中胆碱酯酶（CHE）活力显著（P<0.05）高于试验Ⅲ组,提高了17.70%。④对照组血浆中Mg²⁺浓度与各试验组相比差异不显著（P>0.05）;试验Ⅲ组血浆中Ca²⁺浓度最低,与对照组、试验Ⅰ组、试验Ⅱ组及试验Ⅳ组相比,分别降低了23.44%、16.40%、25.89%及21.43%（P<0.05）。[结论] 综合来看,使用伊维菌素和阿苯达唑混合驱虫,对伊犁马血浆中的氮代谢、糖脂代谢指标及肝功能代谢酶活力改善效果更明显。     

On Noise Control of the Environment in Advanced Living District

The living environment is becoming more and more important,one of the main elements which affect it is noises.This paper takes the Zhonghuafang for example,to research how the whole planning and the designing for the living district affect the control of noises.Applying architectural technology,in order to cut down noises,a sound screen around the site is made and the distinctive constitution for the buildings is designed.The methods of disposing noises are proposed to make sure that the residents of the advanced living district are content with the living environment.     

Study and Practice of Safety Grade Assessment for Large Convention and Exhibition Centre

This paper takes "how to estimate the complex security of Large Convention and Exhibition Centre effectively" as the research goal.Based on analyzing the difficulties,target system,technical constitution,probing into the architecture,safety science,and using for reference the mathematics,operational research,statistics and modern system theory and method,trying to explore establishing an evaluation methodological system,which is provided with integrated characters on subject.Based on these ways,it exerts "quality to quantity" fuzzy analytical hierarchy process(FAHP) and aims at discussing the safety grade assessment of the Chongqing International Conference Exhibition Center focusing on designing theories.The aim is to enhance the integration of design practice and safe consciousness,strengthen the building safety management,and perfect city public security system.In practice,it is also an efficient tool for favoring values of architectural environmental safety.     

黑龙江省农业经济增长方式转变的实证分析

在分析农业经济增长理论的基础上，运用算术指数法得到黑龙江省农业的全要素生产率。依据全要素生产率对黑龙江省农业改革以来的增长方式做了定量描述，从实证分析的角度，考察各种要素投入的增长及其对农业经济增长的贡献，衡量改革以来科技进步在黑龙江省农业生产中的状况和作用，进而描述黑龙江省农业经济增长方式的演进过程，为黑龙江省确定今后加快实现农业经济增长方式转变的政策和战略提供参考依据。     

Quercetin activates mitochondrial autophagy via PINK1/parkin pathway to alleviate cerebral ischemia-reperfusion injury in rats

AIM To analyze the regulatory effect of quercetin （QUE） on PTEN-induced putative kinase 1 （PINK1）/parkin mitochondrial autophagy pathway, and to explore the mechanism of quercetin in relieving cerebral ischemia/reperfusion （I/R） injury. METHODS Sixty SD male rats were randomly divided into sham operation group, model group （I/R group）, QUE group,3-methyladenine （3-MA） group and QUE+3-MA group. Administration started in each group 3 days before modeling, once a day, at 30 min after the last administration,except sham group, the other groups used 4-vessel blockage method to establish the whole brain I/R model. On the day after modeling, the neural function was evaluated by neuropathy disability score （NDS）. The volume of cerebral infarction was measured by 2,3,5-triphenyltetrazolium chloride （TTC） staining. The morphological changes of mitochondria in hippocampus were observed by transmission electron microscopy. The contents of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in hippocampus were measured by ELISA. The activity of superoxide dismutase （SOD） and contents of malondialdehyde （MDA） in hippocampus were detected by xanthine oxidase method, thiobarbituric acid condensation method. Western blot was used to detect the proteinex pression of PINK1, parkin and LC3-II in brain tissue. RESULTS Compared with sham group, the hippocampus of the rats in I/R group and QUE+3-MA group showed swelling of mitochondria, destruction or disappearance of internal crista and other pathological damage,also the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA, the protein expression levels of PINK1, parkin and LC3-II were increased （P<0.05）, while NDS score and activity of SOD were decreased （P<0.05）. Compared with I/R group and QUE+3-MA group, the pathological damage degree of hippocampus in QUE group was reduced, the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA were decreased （P<0.05）, the proteinexpression levels of PINK1, parkin and LC3-II, and NDS score and activity of SOD were increased （P<0.05）.The above indexes in 3-MA group were opposite to QUE group. No significant difference in the above indexes between I/R group and QUE+3-MA group was observed （P>0.05）. CONCLUSION Quercetin activates mitochondrial autophagy and reduces cerebral I/R by regulating the expression of PINK1/parkin pathway proteins.     

菌草灵芝研究现状与前景

以菌草灵芝为试材,从菌草灵芝栽培技术研究、成分提取及分析、免疫学研究、毒理安全、质量安全5个方面系统阐述了菌草灵芝研究现状.结果 表明:菌草灵芝不仅筛选和确定了更适菌草栽培的灵芝菌株和菌草栽培配方,以及不同生长期菌草灵芝有效成分含量,而且采用超声、酶法辅助热水提取多糖、三萜类化合物等菌草灵芝有效成分可提高得率,菌草有效活性成分粗多糖的免疫作用优于纯多糖,且菌草灵芝的毒理和质量安全研究分析表明菌草灵芝无不良反应,质量安全可控,可作为保健产品、美容产品或食品的主要原料或辅料应用于各加工领域.同时,为菌草灵芝进一步深入理论研究提供参考依据.