不同果袋类型对紫花杧的套袋效果

以主栽品种紫花杧为试材,探讨不同果袋类型的套袋效果.结果表明:套袋可大幅度降低采收果的果面流胶和雨水污染,极显著地降低果面虫伤率.套袋可改变采收时的果面颜色,但不同果袋类型间存在差异.后熟完成时,果面均为品种固有颜色,不因果袋种类而改变.套袋能促进果实后熟转黄和果色均匀,能显著降低炭疽病的病果率,但不同果袋对蒂腐病的防效存在较大差异.套袋能提高可溶性固形物含量和减轻果实失重.供试品种用外黄内黑复合纸袋套袋效果最为理想.     

Relationship between genetic instability of nm23H1 gene and clinical pathological characteristic in hepatocellular carcinoma

AIM: The aim of this study was to examine the microstatellite instability (MSI) and loss of heterozygosity(LOH) of locus D17S396 on chromosome 17 and their influence on the expression of nm23H1 in hepatocellular carcinoma (HCC),which may provide experimental evidence for the mechanism of nm23H1 gene and tumor metastasis.METHODS: Techniques such as DNA extraction from formalin-fixed paraffin-embedded tissues,PCR-SSCP,ordinary silver stain were used to study MSI and LOH of locus D17S396.Envision immunohistochemistry and Leica-Qwin computer imaging techniques were used to assess the expression of nm23H1.RESULTS: ① The frequency of heredity instability of HCCs was 35.42%.The frequency of LOH in the cases with lymph node or distant organs metastasis or not and with intrahepatic metastasis or embolus of portal vein or not was significantly different (P<0.01),it was higher in stage TNM Ⅲ than that in stageⅠ and Ⅱ.Moreover,it was higher in high tendency to invasion or metastasis cases than that in the low tendency cases (P<0.01).② The expression of nm23H1 was 56.25%.It was significantly different in Edmondson grade,TNM stage and in lymph node or distant organ metastasis cases (P<0.01).The cases with high tendency of invasion or metastasis exhibited lower nm23H1 expression compared with low tendency cases (P<0.01).③ The positive rate of nm23H1 protein in LOH positive group was lower than that in LOH negative group (P<0.05).CONCLUSION: The results indicate that both MSI and LOH of nm23H1 gene control the development of HCC independently in different pathways.LOH inhibits the expression of nm23H1,which endows it with high aggressive and poor prognosis.Increase in the amount of nm23H1 protein expression effectively restrains the tendency to invasion or metastasis of HCCs and improves prognosis of patients.     

不同钠盐胁迫对高冰草种子萌发的影响

通过对高冰草种子在不同钠盐(NaCl、NaHCO3、Na2CO3)溶液中萌发情况的研究,旨在探明高冰草种子在不同盐胁迫下的萌发特性。结果表明:碱性盐(NaHCO3、Na2CO3)胁迫较中性盐(NaCl)胁迫显著降低了高冰草的发芽率。高冰草种子在NaCl浓度150mmol/L、NaHCO3浓度100mmol/L和Na2CO3浓度50mmol/L时萌发受到明显抑制。不同钠盐胁迫下高冰草种子胚根和胚芽的受抑制大小顺序为,中性盐NaCl胁迫:胚根<胚芽;碱性盐NaHCO3和Na2CO3胁迫:胚根>胚芽。     

啶虫脒防治十字花科蔬菜蚜虫药效试验

蚜虫是为害十字花科蔬菜的主要害虫之一,该虫主要以成虫和若虫群集在蔬菜叶片上吸食汁液,危害后造成叶片卷曲变形,植株生长不良,还排出大量的蜜露、蜕皮而污染叶面,降低蔬菜商品价值.此外,蚜虫还传播多种病毒病.而随着农药的使用量的逐年加大,蚜虫的抗药性逐年增强,常用农药防治效果下降.为了了解啶虫脒对十字花科蔬菜蚜虫的防治效果,找寻高效、低毒、安全的替代农药,特进行本试验.     

传染性法氏囊病病毒抗体检测诊断试剂盒的研制与应用

本文用提纯的ＩＢＤＶ细胞毒为抗原,抗鸡Ｉｇ单抗１Ｂ７ＨＲＰ酶结合物为第二抗体建立了检测ＩＢＤＶ抗体的间接ＥＬＩＳＡ。在此基础上研制出ＩＢＤＶ抗体检测诊断试剂盒。经对１２００份血清进行检测,结果表明：本试剂盒特异性强,灵敏度高,重复性好,快速稳定,易于操作,为雏鸡ＩＢＤＶ母原抗体和疫苗注射后特异性抗体检测提供了可靠的诊断工具。     

民航服务专业中高职衔接模式研究

五年制高等职业教育是职业教育发展新时期的新任务,吉林省教育厅高度重视中高职衔接工作,近年来在教育厅的主导下中高职衔接合作院校与招生规模不断扩大.吉林电子信息职业技术学院积极响应省教育厅的号召,深入开展中高职衔接与合作办学,依靠灵活的办学机制与东方领航教育集团联合办学,探索民航服务专业中高职衔接课题.     

RNAi技术在转基因西瓜抗病毒病上的研究与应用

对RNAi技术的概念、研究进展及西瓜病毒病的危害及其发生特点、RNAi技术在西瓜抗病毒病上的研究进展进行了阐述,并对存在的一些问题作了简要探讨与展望。     

杂交马褂木幼林施肥试验

以杂交马褂木幼林为供试对象，进行不同肥料种类和施肥量试验。结果表明：追肥1a后，各施肥处理间树高生长量有极显著差异，以施洞氮复合肥0.25kg／株和马褂木无机专用肥0.25k∥株的树高生长量最大；追肥3a后，各施肥处理间树高生长量有极显著差异，胸径生长量没有显著差异，以施尿素0.25kg／株的树高生长量最大，胸径和树高生长量分别为3.09em和1.85m，比对照分别提高72％和111％。综合成本效益考虑，适合推广的肥料种类和施肥量依次为马褂木无机专用肥0.1ks／株、马褂木无机专用肥0.25k∥株和尿素0.25kg／株。     

陕南秦巴山区不同立地条件下药用植物多样性研究

通过野外实地调查,研究了陕南秦巴山区不同立地条件下药用植物物种多样性变化和不同生活型物种多样性特征。结果表明:从生活型来看,药用植物乔木层、灌木层、草本层物种多样性指数及物种多样性指数和均表现为:北部山地区东部山地区南部浅山丘陵区;均匀度指数灌木层和草本层均表现为:北部山地区东部山地区南部浅山丘陵区,乔木层则表现:东部山地区北部山地区南部浅山区。总体而言,不同立地条件下药用植物物种多样性表现:北部山地区东部山地区南部浅山丘陵区,即表现海拔越高物种多样性越高的规律。各立地条件下生活型药用植物物种多样性略有不同,南部浅山丘陵区表现为:草本层乔木层灌木层;东部和北部山地区表现为:草本层灌木层乔木层。草本层更具有多变性,能适应不同的生境变化,其多样性最高。