RT-PCR检测猪传染性胃肠炎病毒的研究

根据GenBank发表的猪传染性胃肠炎病毒(TGEV)纤突蛋白S基因的5′端核酸序列设计了1对引物。该对引物扩增的目的片段长度为690bp。在优化RT-PCR反应条件的基础上,建立了TGEV的RT-PCR检测方法。     

利用平衡记分卡加强农机企业的绩效管理

分析了我国农机企业管理中存在的主要问题,并对平衡记分卡的基本原理和作用做了介绍。依据农机企业的特点和现代企业管理理论,阐述了如何在农机企业中建立基于平衡记分卡的绩效指标体系。最后,对相应的绩效考核方法和激励机制做了详细说明。     

高锰酸钾滴定测定乳钙方法的改进

对高锰酸钾滴定法测定乳钙的方法进行了改进；以４％草酸铵直接沉淀乳中钙，以稀氨水洗去多余的草酸铵，再加入０．５ｍｏｌ／Ｌ硫酸使草酸游离，然后以标准高锰酸钾液滴定草酸，间接求出钙含量。２０份乳样用改进的方法和国家标准方法测定结果之间无显著差异（Ｐ＞０．１）；改进方法的重复试验结果的Ｃ．Ｖ％为２．８８％，回收率为（９３．０６±４．８８）％。改进的方法省略了消化过程，使乳钙测定简便化。     

Tetraploid soybeans

1. 1.
   Polyploidy could be induced in four varieties of soybean, both by soaking the seeds in colchicine solution and by treating the apical bud of the germinating seedling through a cotton-wad, the latter being a more successful method. Even though several doses and replicates were tried, the number of polyploids obtained were few and out of four varieties with tetraploid sectors only in the progenies of two of them tetraploid plants were recovered.     

Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis

The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared. In this study, the C. suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C. suis were conducted by iTRAQ labeling and LC-MS/MS. Part of the iTRAQ results were validated by qRT-PCR. Total 174 proteins were identified, among which 40 proteins were differentially expressed, 38 proteins were upregulated and 2 proteins were downregulated. The differentially expressed proteins were mainly involved in metabolism, antibiotics biosynthesis, secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways. The results of this study provide reference for elucidating the developmental mechanism of C. suis sporulated oocyst and facilitated discovering a new biomarker for C. suis.     

Comparing micrometeorology of rain forests in Biosphere-2 and Amazon basin

Micrometeorological variables measured in the BIOSPHERE-2 Center (B2C) enclosed rain forest biome for 1 year were compared with similar measurements made in the Amazon rain forest. In the B2C rain forest, the overlying glass and supporting structure significantly reduces (by approximately a factor of two) the incoming solar radiation. Monthly mean values of above-canopy and within-canopy air temperature, vapor pressure, and vapor pressure deficit are reasonably similar to those of the Amazon rain forest, but there are marked differences in the above-canopy values of these variables in the Arizona summer. Monthly mean diurnal trends also show significant differences. Measurements of vertical air temperature gradient clearly showed two very distinct environments in the 27.4 m high rain forest dome during daylight hours. There is a comparatively cool and fairly well-mixed environment (which is reasonably similar to that found in a natural rain forest) below about 10 m and a hot, thermally stable environment above about 15 m. The nature of the atmospheric turbulence within the B2C rain forest also is significantly different from that normally found in natural rain forests. There is little turbulent mixing above the forest canopy in this enclosed environment. These findings are important for guiding the operation and use of this experimental rain forest facility in future research and for understanding how the rain forest biome functions in an enclosed environment.     

直播早稻覆膜旱作施肥量及密度试验

１９９７年以舟９０３为材料,进行了直播早稻覆膜旱作不同施肥量及密度试验。结果表明：覆膜旱作具有明显的增温作用,从而能提早出苗和缩短余生育期;节能效果明显,直播覆膜旱作可比常规水作法节省氮肥５０％,且产量相当,施用常规水作法７０％的氮肥,则有显著的增产效果。其适宜密度为２０＊２０（ｃｍ）。     

2BHL-4型花生起垄播种机的研制*

按照花生控制下针丰产栽培技术新的农艺要求,设计了全悬挂花生起垄播同,论述了该机的工作过程,结构特点及主要技术性能参数等。