Cloning of Buffalo BMP1 Gene and its Expression Pattern Investigation in Different Tissues

Buffalo BMP1 gene was cloned in the present study, the BMP1 sequence was systemically analysed by bioinformatics techniques, and the expression level of BMP1 gene in different tissues were also assayed with Real-time fluorescence quantitative PCR (QRT-PCR).The results showed that with RT-PCR a 3 195 bp buffalo BMP1 gene was cloned and sequenced, including the whole ORF of 2 967 bp (coding 988 amino acid).The sequence multialigned results showed that buffalo BMP1 gene shared 99%, 96%, 96%, 96% and 95% of similar amino acid sequence with Bos taurus, Sus scrofa, Equus, Homa sapiens and Mus musclus, respectively.It was predicted that buffalo BMP1 protein contained a signal peptide domain, a preregion, a metalloproteinases domain, five complement-Uegf-BMP-1 domain (CUB domain) and two epitheloid growth factor-like domain (EGF-like domain).In addition, we also analyzed the expression level in different tissues through QRT-PCR, the results showed that BMP1 gene mRNA existed in all nine tissues with the most abundant expression in heart, followed by testis, ovary, genital ridge, while with lower amount of bone and other tissues, the minimal expression in liver was observed.     

Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

木薯优良新品种桂垦09-26选育初报

2008年以华南5号为母本,华南205为父本,进行木薯品种间杂交,以选育高产、高淀粉、高抗逆性木薯新品种。当年获得杂交种子936颗,经多代系统选育,于2013年获得综合性状优良的新品系桂垦09-26,鲜薯产量达48.64 t/hm2,比对照华南205增产90.43%;淀粉含量达30.0%,与华南205相近。综合各种农艺性状表现,桂垦09-26可以进一步参加区域性试验和生产性试验。     

桃果实中多菌灵残留量的高效液相色谱-串联质谱结合QuEchERS检测方法

为分析桃果实中多菌灵残留量,采用QuEchERS方法对样品进行前处理,建立了快速检测桃果实中多菌灵残留量的高效液相-串联质谱法。样品经乙腈提取、QuEchERS方法脱水盐析和萃取净化,采用InertsilODS-2(5mm,150mm×2.1mm)色谱柱进行分离,梯度洗脱,在多反应检测模式下测定。经方法学验证,在添加不同浓度的标准溶液时,多菌灵残留量的平均回收率为89.5%~94.4%,RSD为1.95%。该方法简单、快速,准确度高,能够满足多菌灵残留分析的要求。     

高原丘陵区砒砂岩土壤特性的实验分析研究

本文以内蒙古鄂尔多斯高原丘陵区准格尔旗为实验区,用BT-2002型激光粒度分布仪对地表砒砂岩土样机械组成进行分析,得到砒砂岩土样粒径分布的基本特征;并通过滴定及分光光度计对地表砒砂岩的主要物质、化学成分组成进行测试,通过分析整理,对该区域的地表砒砂岩的性质有了清晰的认识,为后续对该地区的水蚀研究提供基础依据。     

非洲凌霄在广州的栽培及园林应用

非洲凌霄(Podranea ricasoliana (Tanf.)Sprague)是紫葳科非洲凌霄属植物，原产非洲南部，近年引入广州栽培，通过近5年试种和繁殖试验，取得较好的效果。文章介绍其形态特征、生态习性、观赏特性、繁殖技术与苗圃栽培，并阐述其在园林中的多种应用方式，为其在广州园林的推广应用提供参考。     

巴西橡胶树乳管细胞HbWRKY1基因克隆及表达分析

采用PCR和RACE技术,从巴西橡胶树无性系热研7-33-97的乳管细胞中克隆了WRKY转录因子家族的一个成员HbWRKY1。该基因全长为1755 bp,含有1个1440 bp阅读框,编码479个氨基酸。生物信息学分析表明,HbWRKY1含有2个WRKY结构域和C2H2锌指结构基序,与蓖麻、杨树、葡萄、黄瓜、拟南芥的WRKY成员的氨基酸序列同源性分别为64.14%、60.04%、42.54%、40.61%和35.4%,属于Ⅰ类WRKY家族成员。实时荧光定量PCR分析表明,割胶和乙烯显著上调HbWRKY1基因的表达,但茉莉酸和机械伤害对HbWRKY1的表达没有明显影响,表明HbWRKY1可能在乙烯信号途径对防卫蛋白的调节中起重要作用。     

BPA不影响卵母细胞减数分裂相关基因Dazl的甲基化

为了探讨环境雌激素BPA对小鼠卵母细胞减数分裂相关基因Dazl甲基化的影响,本研究通过给孕鼠饮用含有BPA的水方式使胎鼠在发育过程中接触BPA,利用重亚硫酸盐测序法,分析了胎鼠生殖嵴卵母细胞不同发育时期Dazl甲基化水平的变化。结果显示:Dazl在减数分裂期间处于低甲基化水平,无论对照组或处理组均低于10%,说明Dazl的低甲基化对维持减数分裂的正常进行有重要作用;对照组与处理组的甲基化水平相当,差异不显著,说明本研究的BPA浓度不影响卵母细胞Dazl的甲基化水平。     

环植所科研主体向海口转移工作与方案研究

中国热带农业科学院环境与植物保护研究所是从事热带农业环境与植物保护领域研究的国家级公益类非营利性研究所。海口环境与植物保护研究实验室项目工程即将竣工，该所科研主体由儋州向海口转移工作已列入所2011年计划的第一项重点工作。从工作方案入手，重点从领导管理、群众参与、宣传引导、督导落实等方面提出建议确保转移工作平稳有序。     

马克思主义生态思想的实践视阈 ——对促进安徽省旅游业可持续发展的启示

马克思恩格斯生态思想是建设社会主义生态文明的理论渊源和行动指南，对于促进资源节约型、环境友好型社会建设有着重要的指导意义。在安徽省旅游业已经进入了转型升级的关键时期，返本开新，深入挖掘马克思主义生态思想的时代精华，对于我们贯彻科学发展观，促进旅游业的可续、健康、快速发展有着不可或缺的启迪作用。