椭圆嗜蓝层孔菌子实体化学成分研究

椭圆嗜蓝层孔菌(Phellinus ellipsoidea)子实体甲醇提取物依次用石油醚和乙酸乙酯萃取,其乙酸乙酯分部经柱层析分离得到11个化合物。经鉴定这11个化合物分别为:苯并(1,2-b,5,4-b′)二呋喃-3,5-二酮-8-甲酸甲酯(1)、原儿茶酸(2)、4-(3,4-二羟苯基)-3-丁烯-2-酮(3)、原儿茶醛(4)、Hispidin(5)、Hispolon(6)、Inoscavin A(7)、Phelligridin K(8)、Inoscavin C(9)、Inoscavin E(10)和Inonoblins B(11),其中化合物2、4、7、10为首次从该物种中分离得到。     

我国马铃薯价格波动规律研究--基于X-12和H-P滤波法的实证分析

马铃薯作为重要的农产品之一,其价格波动受多种因素影响。本文采用X-12模型和H-P滤波法,选取2005年1月至2015年12月全国农产品批发市场价格的月度数据,将其波动分解为季节性、周期性、趋势性和不规则性波动4种类型。结果显示,马铃薯生产的季节性是价格季节性波动的主要原因;成本上升及消费量增加推动价格趋势性上涨但总体趋于平缓;马铃薯价格呈明显的周期性波动,平均周期约为47个月;天气变化、市场突发事件等是价格不规则性波动的主要影响因素。对此,需扎实推进马铃薯生产稳定发展、健全全产业链信息分析预警机制、完善马铃薯价格保险制度,并推进马铃薯一二三产业融合发展,确保薯农收益稳定增长和市场供需基本平衡,促进市场平稳运行。     

Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

Role of type II innate lymphoid cells in browning of white adipose tissue

Type II innate lymphoid cells (ILC2s) are widely distributed in the blood, intestines, trachea, lung, spleen, liver, animal fat and skin, and involved in the innate immune responses. ILC2s have attracted much attention for its important roles in the conversion of white adipose to beige adipose. Studies have shown that ILC2s are essential for the proliferation and differentiation of adipocyte precursor cells, and they also play a vital role in anti-parasitic infection and allergic inflammation. This review discusses the discovery, differentiation, development, distribution and function of ILC2s, and their relationships with the browning of white adipose tissue for providing valuable references on understanding the pathogenesis, prevention and treatment of obesity and fat metabolism disorders.     

后海、后三里、大肠俞对大鼠便秘及胃肠运动神经递质的影响

后海(GV-1)、后三里(ST-36)、大肠俞(BL-25)是治疗动物便秘的常用穴位,本文旨在明确3个穴位对大鼠便秘的缓解作用及其对胃肠运动相关神经递质的调节作用,比较3个穴位作用途径的异同,探索便秘的优势穴位.选取SPF健康大鼠60只,随机分为6组.除对照A组外,其他5组大鼠每只每天灌服3 mg· (kg·d)-1洛...     

一种改进的大麦根尖染色体压片法及其应用

以大麦根尖为实验材料,采用改良的苯酚品红为染色液,在《遗传学实验》的基础上探索适宜的预处理时间、解离时间和染色时间,省略了纤维素酶和果胶酶处理步骤,形成了一套快速、简单的大麦根尖压片方法,该方法获得的染色体图像清晰,便于计数,可用于大麦倍性鉴定和染色体变异方面的研究。     

小麦持久条锈病抗源品种89144(BJ144)芒性状遗传分析

分别以小麦持久条锈病抗源品种89144-2-3-11-2、89144-2-14-4-1-2为父本,感病小麦品种铭贤169为母本进行常规杂交,F1代种子单粒播种,在F2代成株期进行芒的分离遗传分析。结果表明,供试顶芒品系和全芒小麦杂交后,2组合F2代群体芒的性状分离均符合1∶2的理论比例,全芒对顶芒均为显性,且全芒受1对显性基因的控制。这是否说明小麦芒性或顶芒还存在隐性性状的可能,抑或与该小麦材料是外源DNA导入小麦的变异后代有关,需要进一步研究。     

绵羊GP5基因的生物信息学分析

为探讨绵羊血小板糖蛋白V基因(Glycoprotein V,Platelet,GP5)编码蛋白的结构和功能,以NCBI网站的GenBank数据库中发布的绵羊GP5基因序列(登录号为XM₀27965957.1)为基础,采用生物信息学在线工具和软件对绵羊的GP5基因进行生物信息学分析。结果表明,绵羊GP5基因序列中包含1个最大长度为1 620 bp的开放阅读框,推测其编码539个氨基酸,其中亮氨酸含量最多,为22.6%。GP5基因所编码的蛋白其相对分子质量约为59 305.38 KDa,理论等电点为9.40;亚细胞定位结果表明其主要位于内质网(44.4%)。GP5蛋白含有信号肽和跨膜螺旋结构,在二级结构预测中,该蛋白以无规卷曲为主,为70.14%。GP5蛋白三级结构主要由无规卷曲折叠缠绕形成,与二级结构预测结构一致。     

复合有益菌剂对工厂化大菱鲆育苗水净化效果研究

On the basis of purifying effect of single strain probiotic bacteria on water of intensive turbot breeding, the purifying effect of multistrain bacteria product was studied. The results of experiments showed that the free multistrain mixed bacteria had better purifying effect on turbot breeding water than that of any singlestrain bacteria. this phenomenon can be explained with the principle of “ biopump”. The immobilized multistrain probiotics product, biofilm, also showed excellent purifying effect on wate r of turbot breeding. The removal rates of COD and ammonia in 5 days were up to 73.1% and 80.0%, respectively. The concentration of nitrite kept low, which indicated most ammonia had been transformed into nitrate and accumulation of harmful intermediate productnitrite was decreased. This paper supplied theoretical and technical foundation for saving sea water through biopurification of wastewater from marine fish culture.     

不同虾类的过敏原及其过敏原性

近年来食品安全问题受到越来越多人的重视,食物过敏这一食源性疾病已引起广大消费者、生产者和研究者的普遍关注。根据流行病学调查,在欧洲约有2％～2．5％的人受到食物过敏疾病的困扰,婴幼儿的发病率更高,达到4％～6％,儿童约为2％～3％,成年人食物过敏者比例相对较低,但仍有1％～2％。在联合国粮农组织提出的八大类容易引起过敏的食物中,虾蟹等甲壳类动物及其制品是其中重要的一类。我国虽然还没有类似的调查,但有关食用虾蟹等引起过敏的报道屡见不鲜。