鹿茸及鹿血中布鲁氏菌实时荧光定量PCR检测方法的建立

为建立可应用于快速检测鹿茸及鹿血中布鲁氏菌的方法,保证鹿产品的药用、食用安全,试验根据布鲁氏菌特异性基因IS711设计合成引物和探针,建立实时荧光定量PCR方法,对反应条件进行优化,并绘制标准动力学曲线,Y=-3.14X+37.62,R2=0.997.结果 表明:该方法具有良好的重复性、敏感性和特异性,组内、组间重复性...     

烤烟控水节肥育苗措施对水肥利用和烟苗素质的影响

为探明有利于减少水肥投入和降低养分残留的烤烟育苗方法,在烤烟育苗环节以常规水肥管理(CM)为对照,设置了控水节肥模式一(OM1)和控水节肥模式二(OM2)两种控水节肥育苗措施,考察了两个试验点不同控水节肥措施下的水肥利用、肥料残留和烟苗生长状况.结果表明,与常规对照(CM)相比,控水节肥(OM)处理下烟株出苗率、茎高、...     

石榴茎窗蛾生物学特性及防治研究

详述了石榴茎窗蛾的形态特性、生活史及生活习性,试验发现用４０％久效磷乳油３倍液,敌敌畏３倍液和磷化铝毒从排粪孔注入或都忆,防效均达９４％以上。     

Protective mechanism of metallothionein on cultivated rat cardiomyocytes in hypoxic preconditioning

AIM: Studying the mechanism of protective role of metallothionein(MT) in hypoxic preconditioning(HPC) of cultivated rat cardiomyocytes. METHODS:Using the model of hypoxia/reoxygenation of cultivated rat cardiomyocytes. Determining the contents of MT, malonyldialdehyde (MDA)-metabolism product of lipid peroxidation and the activities of Na⁺-K⁺ATPase, Ca²⁺-Mg²⁺ATPase of cardiomyocytes 24h after HPC, also determining the relevant changes after using MT antibody. RESULTS: After 24 h in HPC, the contents of MT and activities of Na⁺-K⁺ATPase, Ca²⁺-Mg²⁺ATPase were obviously higher than those in the control and hypoxia/reoxygenation(P＜0.05), and the contents of MDA were decreased remarkedly (P＜0.01). Then after using MT antibody, the activities of two enzyme were progressively decreased and the contents of MDA were significantly higher than those in the control and MT antibody-free groups(P＜0.01). CONCLUSION: HPC may induce excessive synthesis of MT, and MT can protect myocardial reoxygenation injury by eliminating lipid peroxidation and rising the activities of Na⁺-K⁺ATPase and Ca²⁺-Mg²⁺ATPase.     

Effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits

AIM:To investigate the effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits. METHODS:Test substances were administered intravenously into a marginal ear vein. Temperature responses were measured using rectal thermistor probes. RESULTS:Endotoxin (0.4 μg/kg) stimulated a biphasic rise in colonic temperature. Pretreatment with dimethyl sulfoxide significantly attenuated the biphasic febrile response to endotoxin (0.4 μg/kg) of conscious rabbits in a dose-dependent manner. Rabbits treated with dimethyl sulfoxide 10 min after injection of endotoxin developed fever, which was significantly milder compared with rabbits treated with an equal volume of normal saline. No significant differences were found between thermal response index of rabbits injected with dimethyl sulfoxide and those with normal saline. CONCLUSION:Dimethyl sulfoxide inhibit endotoxin-induced fever in rabbits.     

Role and regulation of calcineurin in angiotensin Ⅱ-stimulated cardiac myocyte hypertrophy of rats

AIM: To study the role and regulation of calcineurin(CaN) in angiotensin II(AngⅡ)-stimulated cardiacmyocyte hypertrophy of rats. METHODS: Using AngⅡ to induce the cultured cardiac myocyte hypertrophy of rats, and investigating the effect of CaN inhibitor on [³H]-leucine incorporation of AngⅡ-stimulated cardiomyocytes and the regulation of various factors on CaN activity in cardiomyocytes.RESULTS: AngⅡ can stimulate the CaN activity in cultured neonatal rat cardiomyocytes in a dose- and time-dependent manner. In cardiac myocytes incubated with 10, 100, 1000 nmol·L^-1 of AngⅡ for 12h, the CaN activities increased respectively by 13%,57%(P＜0.05) and 228%(P＜0.01) compared with that in non-stimulated cardiomyocytes. The CaN activities in AngⅡ-stimulated cardiomyocytes were significantly inhibited by losartan(50 μmol·L^-1), H₇(50 μmol·L^-1)and Fura-2/AM(4 μmol·L^-1),while no effect was observed with PD98059(50 μmol·L^-1).The [³H]-leucine incorporation in AngⅡ-stimulated cardiomyocytes increased by 46%(P＜0.01) compared with that in control group, which was dramatically inhibited by cyclosporin A(0.5~5μg/mL). CONCLUSIONS: Calcineurin, a Ca²⁺/calmodulin-dependent protein phosphatase, may play an important role in AngⅡ-induced cardiac myocyte hypertrophy. The activation of CaN may dependent on the sustained increases of [Ca²⁺]i and be regulated by some protein kinases (such as PKC,etc.).     

Correlation between intraventricular pressure and early phase proto-oncogene expression in volume overload rats

AIM: To investigate the effect of intraventricular pressure change by volume overload (VOL) on expression of proto-oncogene c-fos,c-jun,c-myc and egr-1. METHODS: Left ventricular systolic pressure(LVSP) and left ventricular end diastolic pressure (LVEDP) of rat with VOL induced by aortacaval fistula operation and rats of control group were measured at 30 min, 1, 4, 6, 12 and 48 h after the operation,these mRNAs at the foregoing time points were measured by slot bloting method and quantified with densitometry. RESULTS: Be compared with the control group, VOL rats LVSP decreased (P＜0.05) and declined most remarkably at 48 h,LVEDP elevated significantly at 30 min (P＜0.05), reached a maximal value at 12 h and the levels of control group at 48 h (P＞0.05) after the operation.The proto-oncogene expression signals were not detected in the control,negative controls and VOL rats at 30 min after the operation. The c-fos,c-jun and egr-1 mRNA signals appeared earlier,at 1 h, and c-myc mRNA increased later at 4 h.All reached peak value at 4 h and then declined gradually.The c-fos mRNA were not detected at 48 h. The c-myc,c-0jun and egr-1 mRNA persisted throught the entire observation period from 1 h to 48 h. CONCLUSIONS: During VOL early phase the overload have effect on expression of the proto-oncogene mRNA,c-fos,c-jun and egr-1 mRNA appear earlier, c-myc later,egr-1,c-jun and c-myc persist longer period, but the expressions do not strengthen with the ventricule wall load increase.This sequential induction pattern may reflect the time course regularity of the proto-oncogenes expression induced by VOL,and indicate the proto-oncogenes expression initiate while the heart load accumulate some extent and duration and the load magnitude may not play a critical role.     

Some recent advances in the molecular mechanisms underlying senescence

Aging or senescence is a process in which individuals undergo an exponential decline in vitality, leading to death. Recent years, much progress on the molecular mechanisms underlying senes-cence have been made. (1) Some senescence-related gene such as SEN6A, hic-5, dinl and MORF 4 have been clarified; (2) In 1997, through a set of experiments sponsered scientists of Department of Bi-ology Massachusetts Institute of Technology, it was found that the accommulation of extrachromosomal r DNA circles (ERC) in budding yeast s nucleolus is responsible for cell- senescence and the researchers propose that when enough of these circles accumulate, they clog the nucleus and prevent the cell from reading or eplicating its genome, causing it to stop dividing and ultimately to die; (3) In another work finished National Institute on Aging and the Geron biotech company of Melo, it was proved that a cell s biological clock, which tells the cell how and how many times to divide, lies in its telon eres, little bits of DNA that coat the tips of the chromosome and it was clarified that a powerful enzyme, telomerase, with the potential to rejuvenate the human boss aging tissues could effectively extend the shortened telomere.Although there is a long way to go, scientists still believe that it will be made reality in the future to greatly extend the life-span of human.     

Basic fibroblast growth factor promoting the healing of palatal perforation in rats

AIM: To study the influence of basic fibroblast growth factor(bFGF) on treating ed palatal perforation in rats. METHODS: bFGF was given to the early palatal perforation in rat. The granulation tissues in perforations were grossly and pathologically obserVed. RESULTS: The the of wound healing was significantly in- crease in the bFGF group (P<0.01 ). bFGF could obviously promote the proliferation of the granulation tissues and fibroblasts. The number of AgNORs granules in fibrablasts were 3.73 ±0.52 in the buy group and 2.11 ±0.31 in control group (P < 0.05). CONCLUSION: bFGF could promote the growth of granulation tissues and had a strong promotion on the wound healing in palatal perforation. It was helpful in repairing palatal perforation.