Lenalidomide alone or combined with bortezomib inhibits growth and induces apoptosis in KM3 cells

AIM: To investigate the effects of lenalidomide alone or combined with bortezomib on induction of apoptosis in multiple myeloma(MM) KM3 cells.METHODS: The KM3 cells were treated with lenalidomide alone or combined with bortezomib. The number of viable cells was determined by trypan blue exclusion. Apoptotic cells were simultaneously stained with annexin V-FITC and PI and determined by flow cytometry. RT-PCR was used to examine the level of p65 mRNA. Western blotting was used to examine the protein levels of P65, pP65, caspase-3,-8, -9, and poly (ADP-ribose) polymerase (PARP). RESULTS: Lenalidomide-inhibited the cell growth and induced apoptosis of KM3 cells. The mechanism responsible for lenalidomide-mediated inhibition was via NF-κB and activation of caspase-mediated induction of apoptosis. A synergistic effect was observed when combination of lenalidomide with bortezomib was used. CONCLUSION: Lenalidomide inhibits the growth and induces apoptosis in KM3 cells. There is a synergistical effect when combination of lenalidomide with bortezomib is in use.     

沈阳市水稻生产全程机械化发展探讨

在客观评价沈阳市水稻生产全程机械化发展现状的基础上,从农艺、生产率、机具质量、培训力度、发展水平5方面,详细剖析制约沈阳市水稻生产机械化发展的主要因素,并提出加大政府投入力度、抓好示范区建设、完善机械化信息管理体系等6个切实可行的对策.     

Roles of atypical chemokine receptors in progression and metastasis of tumor

Chemokines and their receptors have been implicated mostly in tumor progression and metastasis. Atypical chemokine receptors (ACKRs) comprise a group of 7-transmembrane domain proteins structurally similar to G protein-coupled receptors. However, ACKRs do not induce classical signaling via the typical G protein-mediated pathways. ACKRs efficiently internalize the cognate chemokine ligands and act as scavengers instead. ACJRs are composed of at least 3 members of chemokine receptors:Duffy antigen receptor for chemokines (DARC, also known as ACKR1), D6 (also known as ACKR2) and ChemoCentryx chemokine receptor (CCX-CKR, also known as ACKR4). These receptors bind to and/or internalize their chemoattractant ligands without activating signal transduction cascades leading to cell migration. In this review, we summarize the recent progress regarding the roles of ACKRs in the progression and metastasis of tumor.     

吉林省不同产地人参中微量元素含量测定

以人参为试材,采用原子吸收光谱法,对吉林省集安市、通化市、临江市、敦化市、蛟河市、桦甸市、抚松县、长白县、靖宇县、汪清县、江源区11个地区采集的生晒参样本进行了微量元素含量测定,其中包括Cr、Mn、Ni、Fe、Zn、Cu、Co和Na元素。结果表明:不同产地之间,除Zn元素外,其余微量元素含量差异均较大。该研究可为人参产地鉴定提供辅助依据。     

我国蜂业是低碳经济产业

和其他种植、养殖业比较而论,我国蜂业属低碳产业。它是利用蜜蜂群体生命活动的本能,生产天然蜂产品,并为植物授粉增加子实量以及维护了植物多样性,是低能耗、低污染、基本上是零排放的、公益性很强的蜜蜂产业。     

基于MSP430F149的便携式温湿度监测仪的设计

为了及时、方便地获取植物病害预测预报等所必需的气象数据,作者设计了一种便携式温湿度气象监测仪,并给出了其硬、软件设计方法。该监测仪以超低功耗单片机MSP430F149、智能传感器SHT15和U盘为主要元器件,能实时地进行温度、湿度和叶面湿度的采集、显示和存储。该监测仪具有体积小、重量轻、携带方便、性价比高和数据存储量大等特点,既可单独使用,也可作为下位机嵌入到系统中实现数据采集和处理的功能。     

水稻OsRFP1基因的原核表达与蛋白纯化

以推导的氨基酸序列分析,水稻OsRFP1基因编码一分子量为34.24kDa锌指蛋白。将OsRFP1基因编码区cDNA序列插入到pGEX4T-3载体中构建OsRFP1-gst融合基因的表达载体,并将质粒转化宿主菌大肠杆菌BL21DE3。经IPTG诱导,融合蛋白在BL21DE3细胞中获得表达。利用亲和层析的方法对融合蛋白进行了纯化,SDS-PAGE蛋白电泳显示获得一60kDa的唯一蛋白条带,即OsRFP1-GST融合蛋白。     

丛枝菌根真菌对番茄植株内源激素含量的影响

于温室盆栽条件下对番茄(Lycospersicon esukurentamu)幼苗接种丛枝菌根(AM)真菌摩西球囊霉(Glomusmosseae)、地表球囊霉(Glomus versiforme)、根内球囊霉(Glomus intraradices)、幼套球囊霉(Glomus etunicatum)或珠状巨孢囊霉(Gigaspora margarita)10d后定期采样,应用间接酶联免疫吸附分析法(ELISA)测定各处理番茄植株根和叶片内源激素吲哚乙酸(IAA)、赤霉素(GA)、玉米素核苷(ZR)和脱落酸(ABA)含量。接种20d G.mosseae处理的番茄根系菌根侵染率高达68.5%,显著高于其他接种处理;供试AM真菌显著增加了番茄植株鲜重、株高、地上部和地下部干重、其根或叶内IAA、GA、ZR和ABA含量均显著高于不接种对照,以G.mosseae处理的根或叶中IAA、GA、ZR和ABA含量最高。     

海水磷酸盐分析方法的优化和试剂稳定性研究

对《海洋调查规范》中测定海水磷酸盐的常规方法进行了优化 ,达到了简化过程 ,缩短分析时间以适应水下现场自动分析的目标。此外 ,实验还对磷酸盐测定所使用的试剂及标准贮备液的稳定性进行了一系列的研究 ,改进试剂保存方法 ,延长试剂使用寿命 ,以满足长期现场测定的要求     

几种杀螨剂对3种叶螨的毒力比较

作者于 1 996年对二斑叶螨 (TetranychusurticaeKoch)、朱砂叶螨 [T .cinnabarinus(Boisdural) ]和山楂叶螨 (T .viennensisZacher)进行了三氯杀螨醇、甲氰菊酯、哒螨酮、霸螨灵等 4种杀螨剂的毒力测定 ,并对 3种叶螨在LC50 和LC95水平上 ,比较了相互之间的毒力差别。结果表明 ,二斑叶螨的毒力最高 ,朱砂叶螨次之 ,山楂叶螨毒力最低。