Relationship between anti-CCP antibody and migration or invasion ability of fibroblast-like synoviocytes from rheumatoid arthritis patients

AIM:To investigate the relationship between anti-cyclic citrullinated peptide (anti-CCP) antibody and migration or invasion ability of fibroblast-like synoviocytes (FLS) from rheumatoid arthritis (RA) patients. METHODS:FLS was isolated from 22 patients with active RA, 12 patients with osteoarthritis (OA) and 6 patients with joint injuries without arthritis history. The migration and invasion abilities were assessed by Transwell assay. RESULTS:Among the patients of RA, OA and normal controls, the numbers of migratory cells were (29.33±10.93), (9.28±7.87) and (7.00±4.07)/field, respectively, and the numbers of invasive cells were (14.35±7.67), (3.96±4.37) and (3.08±1.03)/field, respectively. The numbers of migratory and invasive cells were higher in RA than those in the other groups. The migration and invasion abilities of RA FLS from anti-CCP antibody-positive RA patients were significantly increased as compared with anti-CCP antibody-negative RA patients. Correlation analysis revealed that the migration and invasion abilities of RA FLS were correlated with the positive and high titer of anti-CCP antibody.CONCLUSION: The migration and invasion abilities of FLS from anti-CCP antibody-positive RA patients are increased. Anti-CCP antibody is possibly correlated with the increased migration and invasion abilities of RA FLS.     

低温胁迫下增施锌肥对水稻氮代谢与干物质积累的影响

[目的]施锌是缓解低温胁迫对水稻伤害的有效途径之一,低温胁迫下研究增加施锌量对水稻氮代谢与物质积累的影响,为低温年提高水稻抗低温能力提供理论依据.[方法]采用三叶一心期水稻幼苗进行水培试验,设置低(Zn?0.08?μmol/L)、常规(Zn?0.15?μmol/L)、高(Zn?0.30?μmol/L)?3个ZnSO4·...     

工厂化养鳖——蔬菜水培系统中氮素的转化利用

工厂化养鳖废水加适量硝酸调节后,用于生菜水培系统可以取得良好的净化与利用效果.系统在25 ℃经2周运行,循环液中的总有机氮有85%左右转化为硝态氮,有9%左右被生菜吸收,CODcr与NH4+-N含量分别降至100 mg/L与20～30 mg/L以下,基本上达到排放标准.运行期间,生菜生长良好,重量增长率可达20%～25%.     

富硒女贞子水提物、粗多糖对小鼠免疫的影响

将100只小鼠随机分为5组(雌雄各半),在第1~5天、第8~12天给药组予以相应药物灌服,对照组给予生理盐水,第3、10天用鸡卵清蛋白给小鼠注射免疫,第24天检测相应抗体生成水平和血清中IFN-γ、IL-2、IL-4水平,探讨富硒女贞子水提物、粗多糖对小鼠免疫的影响。结果表明:各给药组的IFN-γ、IL-2、IL-4生成水平均高于对照组,其中组Ⅲ显著高于组Ⅰ、组Ⅱ(P0.05);组Ⅳ公鼠的IFN-γ水平显著高于组Ⅴ(P0.05);与组Ⅰ相比,各给药组卵清蛋白抗体生成水平差异不显著(P0.05)。证明富硒女贞子粗多糖、普通女贞子水提物对小鼠血清中IFN-γ、IL-2、IL-4的生成有明显促进作用,对小鼠的特异性体液免疫作用不明显。     

浅析我国蔗糖研究现状

采用文献计量学的方法.对我国近10年来在期刊上发表的有关蔗糖的文献进行统计分析,着重研究有关蔗糖文献的年限分布、作者分布及研究方向等方面的内容,从而揭示我国蔗糖的研究现状,为我国今后对蔗糖的研究及学术信息交流提供参考依据.     

CPV-Bt对湖南马尾松毛虫的药效试验

以湖南马尾松毛虫发生地采集的马尾松毛虫为试验材料,选用CPV、Bt 2种微生物复配后对其进行室内药效试验,结果表明：Bt对越冬代松毛虫前12d死亡率的影响显著,死亡率与Bt浓度呈正比,Bt浓度为6×10^7个芽孢／mL时,死亡率可达70．36％;Bt使用浓度为5×10^6芽孢／mL时,第一代松毛虫（群体饲养）处理6d平均死亡率可达70．41％,Bt使用浓度为1×10^7芽孢／mL时,平均死亡率可达75．09％;CPV对越冬代松毛虫处理12d后残留种群带毒率影响显著,带毒率与CPV浓度呈正比,当CPV浓度为2×10^5个多角体／mL时,其带毒率可达53．68％;复配药荆按其对第一代松毛虫（单虫饲养）的药效（前6d死亡率）可分为5类,其平均药效（死亡率）最高达100％,最低的仅为43．12％;CPV-Bt复配药刺防治马尾松毛虫的越冬代和第一代,若只需达到70％杀虫率和50％残留种群带毒率水平,越冬代宜选用2×10^5个多角体／mL＋6×10^7个芽孢／mL,第一代宜选用5×10^4个多角体／mL＋1×10^7个芽孢／mL。     

燕麦与苜蓿不同比例组合对驴盲肠体外发酵的影响

研究燕麦干草与苜蓿不同比例组合对驴盲肠液体外发酵的影响,旨在为肉驴养殖中粗饲料的科学利用提供理论依据。将燕麦干草和苜蓿按干物质比为80∶20、60∶40、40∶60和20∶80分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组,通过体外产气法与人工瘤胃持续发酵法培养2、4、8、12和24 h,各时间点3个重复,发酵终止后测定发酵参数和饲草养分降解率,通过24 h时发酵参数的加权估算值计算饲草组合效应值。结果表明:1)燕麦与苜蓿不同比例组合影响了其养分降解率,随着苜蓿比例的增加,干物质(DM)和酸性洗涤纤维(ADF)降解率增大,Ⅳ组显著高于Ⅰ组(P<0.05),各组中性洗涤纤维(NDF)降解率差异不显著(P>0.05)。2)随着苜蓿含量的增加,pH逐渐升高,Ⅳ组显著高于Ⅰ组(P<0.05);各时间点产气量(GP)均为Ⅱ组显著高于其余3组(P<0.05),且24 h时Ⅳ组显著高于Ⅰ和Ⅲ组(P<0.05);从发酵12 h开始Ⅲ、Ⅳ组氨态氮(NH_3-N)浓度显著高于Ⅰ、Ⅱ组(P<0.05);24 h时Ⅱ组微生物蛋白(MCP)浓度显著高于Ⅲ组(P<0.05),与Ⅰ、Ⅳ组无显著差异(P>0.05);燕麦与苜蓿不同比例组合对挥发性脂肪酸(VFA)有显著影响,24 h时Ⅰ和Ⅳ组丙酸浓度显著高于Ⅱ和Ⅲ组(P<0.05),而Ⅰ、Ⅱ组总VFA(TVFA)含量显著高于Ⅲ、Ⅳ组(P<0.05)。3)发酵24 h后Ⅰ、Ⅱ和Ⅳ组均产生正组合效应,且Ⅳ组效应值最大,而Ⅲ组产生负组合效应。综上所述:燕麦与苜蓿按20∶80组合时能提高DM、NDF和ADF降解率,且多项组合效应值更高;燕麦与苜蓿饲喂驴以20∶80组合效果较好。     

Nucleostemin specific RNAi influences cell proliferation in HeLa cells in vitro and in vivo

AIM: To examine Nucleostemin (NS) expression in tumor cells, and observe the effect of NS specific RNA interference on the cell proliferation in Hela cells. METHODS: Total RNA was extracted from 6 kinds of cultured tumor lines, the NS expression level was measured by RT-PCR and Northern blot. An NS-specific siRNA expression vector was constructed to transfect HeLa cell (NS-siRNA-HeLa), and the proliferation of the cell was observed. RESULTS: NS was highly expressed in 6 kinds of tumor cells. NS expression level in the NS-siRNA-HeLa cells was remarkably reduced, and the percentage of G0/G1 cells increased. The neoplasm forming ability in nude mice by the NS-siRNA-HeLa cells was decreased. CONCLUSION: NS is highly expressed among tumor cells. NS-specific siRNA inhibits the entry of the cell cycle into the S phase, and remarkably reduces the proliferation ability of HeLa cells in vitro and in vivo.     

一株水禽养殖废水COD降解菌的分离鉴定及性能研究

从水禽养殖运动场污水中分离、筛选得到1株菌TMS17,经菌落形态特征、生理生化特征鉴定为枯草芽孢杆菌(Bacillus subtilis).枯草芽孢杆菌TMS17可有效降低水禽养殖运动场废水中的化学需氧量(COD).在投菌终浓度为104 CFU/mL、25℃、pH 7.0的条件下进行应用效果试验,结果表明水禽养殖运动场废水48 h的COD降解率达到54.36％,降解后废水达到排放标准.     

表层压缩技术在杨木实木地板生产中的应用

为扩大意杨木材的应用领域、实现高附加值利用,对意杨木材进行表层压缩处理,再将其制成实木地板.对表层压缩意杨木材的性能,及地板成品漆膜性能的检测结果表明:表面压缩处理后,意杨木材表层密度增加;再经热处理后,表面硬度、弹性模量,弦向尺寸稳定性均有增加,但静曲强度略有降低 ;制成的杨木实木地板的漆膜硬度增加.