A pilot study on mechanisms of hyperadiponectinemia in two rat models with different in vivo insulin sensitivity

AIM:To explore the possible mechanisms of hyperadiponectinemia in the insulin-resistant Zucker fatty rats (ZF) and insulin-sensitive nephrotic syndrome rats induced by puromycin aminonucleoside (PA).METHODS:8-week old Zucker rats were fed with standard rat chow for 4 weeks and Wistar rats were intraperitoneally injected single dose of PA 10 d before experiments.Blood samples and 24 h urines were collected for detecting the lipid profiles, adiponectin (ADPN), albumin and urine albumin excretion rate (UAER).A frequently sample intravenous glucose tolerance test (FSIVGTT) was conducted.The insulin sensitivity index (SI) and glucose effectiveness index (SG) were calculated according to Bergmans minimal model.Epididymal white adipose tissues were then extracted for weighting.Correlations between ADPN and other parameters were analyzed by Pearsons correlation.RESULTS:Both ZF rats and PA-injected rats showed significantly higher plasma ADPN levels compared with their own controls ［(7.44±1.23)mg/L vs (2.44±0.33)mg/L and (8.64±0.88)mg/L vs (2.95±0.46)mg/L, respectively, P<0.01］.SI and SG were markedly decreased and negatively correlated to ADPN in Zucker rats, but not changed and correlated to ADPN in Wistar rats.Hyperlipidemia was observed in either ZF rats or PA-injected rats and was positively correlated to ADPN levels.Two hyperadiponectinemic rats showed markedly higher UAER ［(64.8±14.2)mg/24 h vs (14.9±14.6)mg/24 h and (275.1±64.5)mg/24 h vs (15.4± 4.5)mg/24 h, respectively, P<0.01］ and positively correlated with ADPN.The ratio of white adipose tissue to body weight was higher in ZF rats than that in their control rats despite its disproportion with ADPN, while it was comparable between PA-injected and normal rats.The renal creatinine clearance rate was not altered in both strains of rats.CONCLUSION:Hyperadiponectinemia is a common property in ZF rats and PA-induced nephrotic syndrome rats, and it cant be interpreted by changes of in vivo insulin sensitivity.Markedly increased UAER in both rats is the key mechanism of hyperadiponectinemia.     

种茎种植法及芽向对华南205木薯产量的影响

田间试验结果表明:种茎不同种植法对华南205木薯的产量及经济性状没有显著的影响,但平放种植法略优于斜插种植法;种茎芽眼方向对木薯的产量有显著的影响,鲜薯、薯干和淀粉产量均表现为芽眼向南>向东>向西>向北;当采用平放种植法时,木薯产量表现为种茎芽眼向南>向西>向北>向东;当采用斜插种植法时,产量表现为种茎芽眼向东>向南>向西>向北。因此,建议采用起垄、平放、种茎芽眼向南的木薯机械化种植技术。     

转Bt甘蔗遗传多样性与性状分析

采用6对多态性较好的AFLP引物对13个转Bt基因甘蔗品系及其供体品种的遗传多样性进行分析。结果表明:多态性位点率偏低。第1组的多态性位点率为21.00%,第2组多态性位点率为31.80%,总的多态性位点率为42.50%。聚类分析明显把15个品系(种)分为2大类,类间的遗传相似系数为0.54,第1组全部的6个材料聚为Ⅰ类,第2组的9个材料聚为Ⅱ类。外源Bt基因的导入可在一定程度上增加种质的多样性,但增加幅度不大。虽然转Bt基因甘蔗品系的遗传多样性不高,但性状变化比较明显,经济性状普遍变差,抗虫性可分为1～5级。     

鸡病毒性关节炎病毒S1133株在鸡胚成纤维细胞培养传代效果观察

鸡病毒性关节炎病毒Ｓ１１３３株，在鸡胚成纤维细胞培养传代，至第６代后，细胞产毒效价明显提高，已达到了鸡培养的产毒水平。这为开发细胞培养工艺提供重要依据。     

基于不变矩和神经网络的常用电气符号自动识别方法研究

工程图纸中电气符号的自动识别是实现电气工程图纸矢量化的基础。提出了提取工程图纸中电气符号的不变特征。把不变特征值输入神经网络进行训练，得到神经网络模型识别旋转、平移、缩放电气元件图元的方法。实验结果表明：所建立的网络模型具有较好的性能。将此方法用于工程图纸的矢量化处理过程，只需识别出电力工程图纸中各个符号的类型，避免了图纸矢量化过程中的跟踪和拟合的过程，因此优化了矢量化流程。     

基于图像处理的葡萄叶片含水率诊断初探

随着图像处理与分析技术的蓬勃发展,许多专家学者利用图像获取工具比人眼更精细的分辨能力,应用图像处理技术进行信息诊断研究。以温室大棚中的葡萄植株为研究对象,使用卡西欧ex-2750数码相机采集葡萄冠层叶片图像,并通过烘干法测量叶片样本的含水率,通过MATLAB计算叶片图像的灰度均值。通过对提取的15组葡萄叶片样本数据的多项式曲线拟合,建立了基于叶片图像灰度均值的叶片含水率估算模型,模型的确定系数R2高达0.780 3。结果表明,利用叶片图像的灰度均值可以对葡萄叶片的含水率进行诊断。     

基于多路复用的群集邮件系统的研究与实践

在分析目前高校邮件系统面临问题及传统解决方案存在不足的基础上，讨论了实现横向可伸缩性，实现可用于连接到多个邮件服务器的单一域名，以使整个邮件服务器阵列作为一个单一主机呈现给邮件用户的多路复用器技术，并给出了中国农业大学基于此技术的群集邮件系统的应用实践。     

宁波市杨梅虫害种类及其发生规律

连续3a对慈溪、余姚不同杨梅林分的害虫种类调查及分类鉴定，结果表明，为害杨梅的害虫共有30种分属5目20科26属。杨梅各害虫因不同的生物学特牲而在林间表现出不同的时空(时间和空间)规律。     

Apoptosis in diabetic foot ulcers and human fibroblast cells treated with AGEs

AIM：To investigate cell apoptosis in diabetic foot ulcers and the effect of advanced glycosylation end products (AGEs) on apoptosis in human fibroblast cells. METHODS：Diabetic foot patients (n=18) and 18 age-matched non-diabetic controls were recruited. The clinical and biochemical features were compared by statistics methods. Skin biopsies were obtained from foot. Cleaved caspase-3 was measured by immunohistochemistry using the technique of streptavidin-biotin complex (SABC) staining. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) technique was used to detect apoptosis of the skin tissues. Human primary foreskin fibroblasts were isolated and cultured in the presence of 5.6 mmo/L glucose, 25 mmo/L glucose, fluctuant glucose (changing the glucose from 5.6 mmo/L to 25 mmo/L every 8 h) or AGEs (150 mg/L, containing 5.6 mmo/L glucose). After 72 h treatment, Western blotting was used to determine the levels of the apoptotic protein cleaved-caspase-3. Other cells were trypsinized, washed with cold PBS and incubated with PI and Annexin V-FITC, then analyzed by flow cytometry to detect cell apoptosis. RESULTS：Diabetic patients had higher levels of fasting blood glucose (FBG), 2-hour postprandial blood glucose (2 h PBG) and glycosylated hemoglobin A1c (HbA1c), and longer wound duration. The protein level of cleaved caspase-3 was significantly higher in diabetic group, suggesting that apoptosis was increased in diabetic skin tissues. TUNEL analysis showed that apoptotic index was higher in diabetic group compared with that in non-diabetic group (8.4%±1.5% vs 3.8%±08%), which further confirmed that cell apoptosis was increased in diabetic foot tissues. In human fibroblasts, the levels of cleaved caspase-3 in normal group, sustained high glucose group, fluctuant high glucose group and AGEs group were 080±0.13, 1.22±0.18, 1.46±0.32 and 1.83±0.25, respectively. The apoptotic rates detected by flow cytometry were 2.43%±0.19%, 2.89%±0.51%, 3.99%±0.24% and 6.83%±0.36%, respectively. Both the level of cleaved caspase-3 and the apoptotic rate in AGEs group were higher than those in normal glucose group and sustained high glucose group. CONCLUSION：Increased apoptosis in diabetic foot ulcers is one of the most important reasons for impaired wound healing. As compared to sustained high glucose and glucose fluctuations, AGEs induce greater apoptosis in human fibroblast cells.     

平衡施肥对甘蔗产质量及效益的影响

试验结果表明,平衡施肥能促进甘蔗生长发育,改善经济性状,提高产量、糖分含量及经济效益,降低生产成本。平衡施肥的甘蔗产量达119701.0kg/hm2,比常规施肥的增产11757.8 kg/hm2,增长10.9%;糖分含量提高0.28%,产糖量19271.9 kg/hm2,比常规施肥的增产2216.9 kg/hm2,增长13.0%,降低生产成本520.0元/hm2,净增效益47917.7元/hm2,比常规施肥的增收5811.1元/hm2,增长13.8%,效果显著。