标记抗体染色法检查布氏杆菌的研究

为了建立一种快速、简便、敏感、特异地检测动物病理材料中布氏杆菌的方法,选用四种标记抗体法,对5种共15个生物型的布氏杆菌,以及大量与流产有关或常常污染病理材料的对照细菌,分别进行了纯菌、感染实验动物及牦牛组织材料的染色检查,并与柯氏染色法及细菌分离培养法进行了比较。结果表明,四种方法对于各种材料中的布氏杆菌(粗糙型犬布氏菌除外)均呈阳性反应,而绝大多数对照菌均呈阴性反应。其中尤以SPA法染色背景更为清晰,非特异性反应更少。这些方法既简便、快速,检出率也较高。对于布氏杆菌病的诊断和疫情监测,是很有价值的。     

双孢菇的营养价值及栽培技术

1营养价值及保健功能双孢菇具有食用、保健功效。蘑菇的菌肉肥嫩,味道鲜美,营养丰富。每100g鲜菇中含蛋白质3.7g,脂肪0.2g,糖3.0g,纤维素0.8g,磷110g,铁0.6mg,灰分0.8g,VC 3mg,VB10.1mg,VB20.35mg,烟酸149mg。蘑菇既是一种高档的菌类蔬菜,又是一种有保健作用的营养食品。含高蛋     

绢丝昆虫茧丝蛋白酶抑制剂含量的比较分析

以 6种绢丝昆虫的茧壳为材料 ,对茧丝蛋白酶抑制剂的含量及热稳定性进行了研究。结果表明 :家蚕品种间茧丝蛋白酶抑制剂的含量差异极显著 ,外层丝中含量较多 ,F1代的含量呈双亲中间型 ,偏向于含量较多的亲本 ,雌雄间含量无显著差异 ;家蚕茧丝蛋白酶抑制剂对热稳定性较强 ;天蚕绿茧、野蚕的茧丝中有少量的蛋白酶抑制剂存在 ,蓖麻蚕的茧丝中有微量蛋白酶抑制剂存在 ,天蚕黄茧、柞蚕茧、樗蚕茧的茧丝中基本无蛋白酶抑制剂存在。     

Vaccination of the brushtail possum (Trichosurus vulpecula) against Mycobacterium bovis infection with bacille Calmette-Guérin: the response to multiple doses.

In New Zealand, the brushtail possum (Trichosurus vulpecula) is the principal wildlife vector of bovine tuberculosis. Control of infected possum populations contributes to the control of tuberculosis in domestic livestock. Vaccination is potentially a complementary strategy to population control, but to be cost-effective, administration of the vaccine to possums would need to be from an appropriately designed automatic vaccinator. Possums themselves would activate the vaccinator so that it would deliver an aerosol spray of vaccine. There would be no direct way to prevent possums receiving multiple doses of vaccine. This study examined the effect on protective immunity of repeated vaccination. Captive possums were vaccinated with BCG strain pasteur 1173P2 either 12 times at weekly intervals, twice at 6-weekly intervals, or once. Vaccination was by a combination of intranasal aerosol and conjunctival instillation. Eight weeks after the last dose of vaccine, all possums were challenged intratracheally with Mycobacterium bovis strain 83/6235. Vaccination induced a significant immune response as measured by the lymphocyte proliferation assay (LPA). A significant level of protection, as measured by the response to challenge, developed in all the vaccinated possum groups, but protection was greatest in the group vaccinated 12 times. It was concluded that protection would be enhanced if vaccinations were repeated at short intervals (weekly), but no benefit or detriment resulted from revaccination after longer intervals (1-2 months).     

氟砜霉素和氯霉素与抗菌增效剂的体外联合抑菌效果比较

应用试管二倍稀释法测定了氟砜霉素、氯霉素、TMP和OMP对15株畜禽常见病原菌的最小柳菌浓度（MIC）。结果表明，氟砜霉素的体外抗菌活性强，优于或等于氯霉素；TMP和OMP的体外抗菌活性偏低，但OMP优于TMP。氟砜霉素和氯霉素与TMP、OMP对14株细菌的联合药敏试验经肉汤稀释棋盘法测定，结果表明，氟砜霉素和氯霉素分别与TMP、OMP体外联合，抑菌效果相同，呈协同作用的均占50%与28.6%，即TMP或OMP对氟砜霉素和氯霉素的增效作用相等，但TMP优于OMP。在14株细菌对氟砜霉素和氯霉素分别与TMP、OMP的总共56组体外联合试验中，呈协同作用的占39.3%，主要为大肠杆菌、多杀性巴氏杆菌、沙门氏菌及金黄色葡萄球菌；无关作用的点60.7%；无拮抗现象。故临床联合用药前，仍需进行联合药敏试验，呈协同作用者才可联用。     

Pharmacokinetics and pharmacodynamics of butorphanol in llamas after intravenous and intramuscular administration.

OBJECTIVE: To evaluate disposition of butorphanol after i.v. and i.m. administration, effects on physiologic variables, and analgesic efficacy after i.m. administration in llamas. DESIGN: Nonrandomized crossover study. ANIMALS: 6 healthy adult male llamas. PROCEDURE: Butorphanol (0.1 mg/kg [0.045 mg/lb] of body weight) was administered i.m. first and i.v. 1 month later. Blood samples were collected intermittently for 24 hours after administration. Plasma butorphanol versus time curves were subjected to pharmacokinetic analysis. Two months later, butorphanol (0.1 mg/kg) was administered i.m., and physiologic variables and analgesia were assessed. RESULTS: Extrapolated peak plasma concentrations after i.v. and i.m. administration were 94.8 +/- 53.1 and 34.3 +/- 11.6 ng/ml, respectively. Volume of distribution at steady state after i.v. administration was 0.822 +/- 0.329 L/kg per minute and systemic clearance was 0.050 +/- 0.014 L/kg per minute. Slope of the elimination phase was significantly different, and elimination half-life was significantly shorter after i.v. (15.9 +/- 9.1 minutes) versus i.m. (66.8 +/- 13.5 minutes) administration. Bioavailability was 110 +/- 49% after i.m. administration. Heart rate decreased and rectal temperature increased. Somatic analgesia was increased for various periods. Two llamas became transiently sedated, and 2 became transiently excited after butorphanol administration. CONCLUSIONS AND CLINICAL RELEVANCE: Although i.v. administration of butorphanol results in a short half-life that may limit its analgesic usefulness, the elimination half-life of butorphanol administered i.m. is likely to be clinically useful. The relationship among plasma butorphanol concentration, time, and analgesia differed with the somatic analgesia model; clinically useful analgesia may occur at lower plasma concentrations than those reported here.