FC‐14 Clonality studies of feline cutaneous lymphocytosis

A previous study described cutaneous lymphocytosis (CL) in 23 cats. The process resembles cutaneous pseudolymphoma in humans, a heterogeneous group of benign reactive proliferations of well‐differentiated lymphocytes in the skin of humans. Morphological and immunophenotypic characteristics do not offer reliable criteria to accurately predict the clinical outcome of feline CL or pseudolymphoma in humans. Presence of clonal cell populations is more consistent with a neoplastic process. In a previous study, feline CL lesions (20 cats) were evaluated for clonality using PCR, and only two cats had monoclonal T‐cell populations. Because false‐negative results may occur, the purpose of this study was to repeat the PCR using a revised primer set based on analysis of additional feline T‐cell receptor γ (TCRγ) sequences. DNA was isolated from 29 skin lesions and six internal organs of 20 cats. DNA integrity was assessed by glyceraldehyde‐3‐phosphate dehydrogenase PCR. Polymerase chain reaction clonality was performed using the revised primer set specific for feline TCRγ, and duplicate samples were evaluated. The PCR products were assessed by heteroduplex analysis. Clonal rearrangement of TCRγ was detected in 14 cats (24 of 35 tissues: 21 of 29 skin lesions and three of six internal organs); eight of these cats are still alive and six were euthanized. Monoclonal populations were seen in three of five cats that had involvement of internal organs. These findings indicate that feline CL is best considered as a slowly progressive process which may be reactive, but often evolves into a low‐grade indolent lymphoma. Funding: George H. Muller Fund for Research in Dermatology.     

Epidemiological Study of Non‐contagious Intramammary Infections in Nine Commercial Dairy Herds following a Staphylococcus aureus Control Programme

Changes in prevalence in intramammary infection, by pathogen type, in herds applying a stringent contagious mastitis control programme was studied. Enrollment of 1651 lactating cows and collection of milk samples was made in this ancillary study to a cohort study of the dynamics of mastitis prevalence after adoption of a strict contagious mastitis control programme that targeted the elimination of mastitis caused by Staphylococcus aureus. Nine commercial dairies in Italy were used. Aseptic collection of milk samples from all lactating cows was performed at the time of enrollment, from all cows within 7–14 days of entering the lactating herd after the date of enrollment, and from all lactating cows at 2, 4, 7, 10 and 14 months after the date of enrollment. Prevalence of intramammary infection by pathogen type was determined from culture of milk samples. Application of the strict contagious mastitis programme did not lead to an increased risk of non‐contagious mastitis. The risk of coliform, environmental streptococcal and coagulase‐negative staphylococcal intramammary infections decreased after adoption of the programme. The data reported herein indicate that the overall risk for any intramammary infections decreases with adoption of a strict contagious mastitis programme, and that such a programme therefore does not necessarily lead to an increase in environmental mastitis.     

Germination ecology of seeds of the annual weeds Capsella bursa-pastoris and Descurainia sophia originating from high northern latitudes

Low temperatures may inhibit dormancy break in seeds of winter annuals, therefore it was hypothesized that seeds of Capsella bursa‐pastoris and Descurainia sophia that mature at high latitudes in late summer–early autumn would not germinate until they had been exposed to high summer temperatures. Consequently, germination would be delayed until the second autumn. Most freshly matured seeds of both species collected in August and September in southern Sweden were dormant. After 3 weeks of burial at simulated August (20/10°C) and September (15/6°C) temperatures, 28 and 27%, respectively, of the C. bursa‐pastoris and 56 and 59%, respectively, of the D. sophia seeds germinated in light at 15/6°C. In contrast, in germination phenology studies conducted in Sweden, only a few seeds of either species germinated during the first autumn following dispersal. However, there was a peak of germination of both species the following spring, demonstrating that dormancy was lost during exposure to the low habitat temperatures between late summer and early autumn and spring. Nearly 100% of the seeds of both species subjected to simulated annual seasonal temperature changes were viable after 30.5 months of burial. In the burial study, exhumed seeds of C. bursa‐pastoris were capable of germinating to 98–100% in light at the simulated spring–autumn temperature regime (15/6°C) in both spring and autumn, while those of D. sophia did so only in autumn. In early spring, however, seeds of D. sophia germinated to 17–50% at 15/6°C. Thus, most seeds of these two annual weeds that mature in late summer do not germinate in the first autumn, but they may do so the following spring or in some subsequent autumn or spring.     

Evaluation of sampling methods and assessment of the sample size to estimate the weed seedbank in soil, taking into account spatial variability

As field sampling is time consuming, it is necessary to develop efficient sampling techniques to obtain accurate estimates of the weed seedbank in soil. The relative efficiency between sampling schemes depends on the spatial variability in weed seed density across agricultural fields. Spatial variability of the weed seed density was characterized by theoretical correlograms. A systematic sampling (square grill) scheme was considered and it was found that, taking into account spatial variability, this sampling scheme was more efficient than simple random sampling. As a result, the sample size can be reduced in comparison with that given in previous studies, where spatial correlation was ignored. The reduction depends on the correlation structure defined as a function of the ratio, τ, between the nugget effect and the sill of the variogram. The maximum reduction of the sample size, without loss of either precision or confidence level corresponds to the case where there is no nugget effect, τ = 0. The opposite extreme case, where the reduction is nil, corresponds to the case of a pure nugget effect τ = 1. The abaci based on given expressions are provided to determine the sample size in species whose spatial pattern can be fitted either to a Poisson or to a negative binomial distribution.     

Spatial distribution of Lolium rigidum seedlings following seed dispersal by combine harvesters

This paper considers the relationships between the dispersal of seeds and the distribution pattern of an annual weed. A comparative study of seed dispersal by combine harvesters, with and without a straw chopper attached, was established using Lolium rigidum, a common weed in Mediterranean cereal crops. Seed dispersal distance was quantified and the relationships between dispersal and fine‐scale seedling distribution evaluated. Primary dispersal of L. rigidum seeds occurs in a very limited space around the parent plants, but the density of seed is low because most seeds do not fall from spikes spontaneously. In contrast, many seeds are spread by combine harvesters. In this study the maximum dispersal exceeded 18 m from established stands in cereal fields, although the modal distance was close to the origin. In addition, the action of the combine harvesters tended to accumulate L. rigidum seeds predominantly under the straw swath, with some lateral movement. This action could explain the fine‐scale banded pattern of L. rigidum in cereal fields. Although the treatment of straw by the standard and straw chopper combines differed, the resultant seed distribution showed few differences.     

Fireblight monitoring in Lithuania1

Lithuania has requested that its whole territory should be recognized by the EU as a protected zone for Erwinia amylovora . Fireblight monitoring was performed in 1998/2002 with the aim of detecting and identifying the bacterium, and of determining its distribution in the country. The study consisted of periodic surveys (at least twice a year) of nurseries, orchards, collective farms and host plants, growing individually or in small groups, as well as the surrounding zone within a radius of 250 m. Tests, under conditions of quality control, were applied to host plants with and without symptoms, using detection methods such as ELISA and immunofluorescence (with polyclonal antibodies), semi-selective plating and pathogenicity.     

Expression of the tobacco β-1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR.     

Comparative studies of acetylcholinesterases purified from two field populations of the oriental migratory locust (Locusta migratoria manilensis): implications of insecticide resistance

Acetylcholinesterase (AChE) was purified by affinity chromatography from two populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from Huanghua and Pingshan Counties, Hebei Province of China. The purification factors and yields were 1661-fold and 19.3%, respectively, for the Huanghua population, and 3897-fold and 39.6% for the Pingshan population. Both the purification factor and yield were significantly lower in the Huanghua population than in the Pingshan population. AChE activity was almost completely inhibited by 10⁻⁶ M eserine and BW284C51, but ?5.8% of AChE activity was inhibited by ethopropazine at the same concentration, suggesting that purified AChE from either population was a typical insect AChE. However, AChE purified from the Huanghua population was 62-, 2.0-, and 1.6-fold less sensitive to inhibition by the three organophosphate compounds, chlorpyrifos oxon, demeton-S-methyl, and paraoxon, respectively, than that from the Pingshan population. Significantly lower purification factor and low yield associated with reduced sensitivity of AChE to inhibition by the organophosphates indicated that AChE purified from the Huanghua population was biochemically and pharmacologically different from that of the Pingshan population. Reduced sensitivity of AChE appeared to contribute to organophosphate resistance in the locust from Huanghua County, where insecticides have commonly been used to manage outbreaks of the locust.     

Effectiveness of the resistance inducer prohexadione‐Ca against fireblight in shoots of apple trees inoculated with Erwinia amylovora1

Due to the lack of effective, non‐phytotoxic and publicly acceptable materials for controlling fireblight in pome fruit trees, novel strategies against Erwinia amylovora are being sought. Resistance‐inducing compounds, such as prohexadione‐Ca, represent promising alternatives. Prohexadione‐Ca is the active substance of the bioregulator Regalis, currently being introduced in several European countries and overseas. Prohexadione‐Ca reduces shoot elongation due to inhibition of gibberellin biosynthesis. Furthermore, it leads to significant changes in the spectrum of flavonoids and their phenolic precursors in pome fruits, which causes reduced susceptibility to fireblight and other pathogens. In 2002 and 2003, container‐grown apple trees of the cultivars ‘Idared’ and ‘Freedom’ were treated with different dosages of prohexadione‐Ca two weeks before inoculation with E. amylovora. The effect of prohexadione‐Ca against shoot blight was determined by measuring the lengths of necrotic lesions and symptoms on vascular bundles caused by the pathogen. Treatments with prohexadione‐Ca turned out to be much superior to the ones with streptomycin, kasugamycin and a bacterial antagonist, which were used for comparison. Acibenzolar‐S‐methyl (Bion), another resistance‐inducing compound, was included in some of the experiments and gave intermediate results. The simultaneous control of excessive shoot growth and shoot infections by fireblight is seen as a major advantage of using prohexadione‐Ca in pome fruit trees.     

Differences in the growth stages of Erysiphe pisi on cultivars of field pea (Pisum sativum L.)

Journal of Plant Diseases and Protection - The infection process of Erysiphe pisi on field pea cvs. ‘Highlight’ and ‘Tara’ carrying resistance gene erl, line...