Effects of Added Substrate on Production of Red Swamp Crawfish, Procambarus clarkii, in Earthen Ponds without Planted Forage

The effects of addition of substrate consisting of plastic mesh fencing to experimental earthen ponds were evaluated for the culture of the red swamp crawfish, Procambarus clarkii, without planted forage. Mean daily yield (kg/ha/d), number of crawfish harvested (number/ha/d), and mean individual harvest weight were compared for ponds with and without substrate during a 2‐yr period. The surface area of added substrate was equivalent to 50% of the bottom surface area of the pond. Mean daily yield in the ponds containing substrate, based on a comparison with that of the previous year, increased by 80.2% and was principally attributed to a 66.7% increase in the number of crawfish harvested. Comparative values for the ponds without substrate were ?13.1 and ?11.7%. For the second year, the treatment‐dependent response was consistent. Mean daily yield for the substrate ponds increased by 48.8% compared to 15.0% for the ponds lacking substrate. The primary benefit of the substrate appears to be provision of refuge and three‐dimensional distribution of crawfish in the pond, thereby reducing the incidence of cannibalism and increasing the total number of crawfish harvested per hectare per day. The benefit of the substrate in a commercial production setting must be weighed against its cost and longevity as well as the conditions of the particular management scenario into which it may be incorporated.     

猪圆环病毒和猪繁殖与呼吸综合症混合感染的诊治

2002年4月 ,我省某大型规模猪场发生以母猪流产、死胎、弱仔及断奶仔猪发病率、死亡率高为基本特征的疫病 ,经流行病学调查、临床及病理剖检、实验室检验 ,诊断为猪圆环病毒病 (PMWS)和猪繁殖与呼吸综合症 (PRRS)混合发病 ,采取综合防治措施后 ,疫情得到一定控制。现报告如下 :1猪场基本情况该场常年存栏母猪近800头 ,存栏生猪7500多头 ,每月平均产140窝 ,1350 -1450头仔猪 ,年出栏肉猪10000头以上 ,并提供部分种猪。猪瘟免疫程序如下 :种公猪每年春秋2次 ,母猪则在每次配种前免疫 ,每次4头份 ,初生仔猪哺乳前首免 ,次免于50日龄进行 ,…     

优质面包小麦新品种9507引种试验

中优9507是中国农科院作物所育成的强筋力优质面包小麦新品种.该品种1997年秋引入我县试种,1997～2000年通过天津市冬小麦区域及生产试验,1999年被列为农业科技跨越计划核心品种,2000年3月通过市审定.     

小麦面粉沉淀值的微量快速测定(简报)

沉淀值(sedimentation value)是衡量小麦面粉蛋白质品质的一个重要指标。研究表明,沉淀值与面包的体积及面包得分有最密切的相关性。可用沉淀值测定法来估价小麦面粉的品质。许多育种单位都采用沉淀值测定法作为蛋白质品质育种的早代筛选法。按照 ICC-116标准,沉淀值测定需要最少3.2g 面粉,如果要在 F_2对单株进行测定,样品量显得大了些。因而有人发展了一些微量的沉淀值测定法。作者在联邦德国进修期间,对一私人育种公司发展的一种微量测定法进行了研究,现将结果简报如下。     

Flow cytometric analysis of intracellular complexity and CD45 expression for use in rapid differentiation of leukocytes in bovine blood samples.

OBJECTIVE: To develop an efficient and reliable method that accurately differentiates bovine lymphocytes from monocytes in leukograms. SAMPLE POPULATION: Blood samples from 30 healthy cows and 1 calf with bovine leukocyte adhesion deficiency. PROCEDURE: Flow cytometric analysis of intracellular complexity and CD45 expression on bovine leukocytes was compared with results for conventional light microscopy methods. Verification of leukocyte subpopulations determined by intracellular complexity and CD45 expression was conducted, using 2-color phenotypic analysis with selected monoclonal antibodies. RESULTS: The CD45 and side-scatter properties of bovine leukocytes clearly differentiated cell types, including neutrophils, eosinophils, monocytes, and lymphocytes. CONCLUSIONS AND CLINICAL RELEVANCE: This is a rapid assay that is simple to use. More importantly, it is more accurate than the conventional method that involves the use of blood slides and light microscopy, because of the ability of the assay to readily distinguish bovine monocytes and lymphocytes. Rapid preparation of samples and short analysis times allow for efficient and reliable examination of a large number of samples, and the task of viewing slides by light microscopy is eliminated. The labor-savings benefit of this procedure is most apparent in research environments that require frequent processing of batches of blood samples.     

沙门氏菌鞭毛蛋白属共同抗原表位的定位

用淋巴细胞杂交瘤技术研制成４株沙门氏菌属特异单抗ＣＢ８，ｄｅ７，ｃｃ６，ＤＤ４，在免疫转印试验中，证实它们所识别的抗原表位位于鞭毛蛋白上，ＥＬＩＳＡ相加试验及竞争试验显示，ＣＢ８和ｃｃ６与ｄｅ７，ＤＤ４识别的抗原表位不同，表明鞭毛蛋白的属共同抗原具有多种表位的特性。用胰蛋白酶消化鞭毛蛋白，ＳＤＳ－ＰＡＧＥ结果出现两条新带，分子量分别为４２０００（Ｆ４２），２７０００（Ｆ２７），长时间消化，最终只剩下Ｆ２７条带，它可抵制该酶的消化。对该酶切样品的免疫转印结果表明，沙门氏菌鞭毛蛋白属共同抗原表位出现于Ｆ４２和Ｆ２７上，而相应的沙门氏菌分型Ｈ抗原单抗或因子血清识别的表位也在Ｆ４２和Ｆ２７上都得到了证实。这为沙门氏菌鞭毛蛋白抗原的多样性及结构和功能研究提供了证据。     

加工现场微机控制系统抗干扰的研究

针对制造业加工现场的环境特点，分析了微机控制系统的干扰来源和途径，介绍了实用的抗干扰措施。实践证明：这些软硬件抗干扰措施的综合运用，使自动化加工中微机控制系统的稳定性得到了较大的提高。