A pulmonary granular cell tumour with associated hypertrophic osteopathy in a horse

Abstract Extract A 15-year-old pony mare developed firm irregular bony swellings on all four legs. These were most severe around the carpal and fetlock joints of the forelegs, restricting both flexion and extension. Over about 6 months the horse had periodic bouts of coughing. She showed moderate weight loss and a depressed demeanour.     

Rotaviral diarrhea in pigs: brief review.

Rotavirus is a name given to a group of viruses that have similar characteristics and are generally capable of causing diarrhea in the young. Infection of pigs with porcine rotavirus is common and widespread and can result in diarrhea, especially in 1- to 4-week-old pigs. This virus is frequently associated with a diarrheal syndrome popularity known as "white scours," "milk scours," or "3-week-old scours." Pigs less than 1 week old are infrequently infected, presumably because of adequate passive immunity. The infection resembles enzootic transmissible gastroenteritis. Diagnosis can be made by immunofluorescent staining of mucosal scrappings from the small intestines.     

Infection pressure of potato virus YN

Potato virus Y^N (PVY^N) infection was determined by the tobacco test in Swifterbant (Eastern Flevoland). In plots with beet, wheat and seed potatoes the infection exhibited an identical course. No differences were found either between PVY^N infection in the border and that in the middle of a field planted with ware potatoes, although infection pressure was clearly higher here than in the plot with seed potatoes. A barrier crop of 10 rows of wheat did not decrease the infection pressure of the virus.From August onwards, the spread of PVY^N in Lienden (Betuwe) was followed. Here virus transmission was found continuously, even until mid-November.Potato volunteers outside as well as in potato fields are serious infection sources. In 1976 and in 1977 virus spread was detected before the flight ofMyzus persicae, as determined with yellow Moericke traps. Infection pressure can be measured more efficiently by the tobacco test than by aphid trapping. The effect of rogueing at the time of virus spread should be reconsidered.If infection pressures in different areas or successive years are to be compared, the tobacco test should be standardized. A proposal to this effect is made.Samenvatting In Swifterbant (Oostelijk Flevoland) werd de infectie met het aardappel-Y^N-virus (PVY^N) bepaald met behulp van de tabakstoests. In percelen met bieten, tarwe en pootaardappelen bleek de infectie hetzelfde verloop te hebben. Tussen het infectieverloop van PVY^N in de rand en in het midden van een veld consumptieaardappelen werd eveneens geen verschil gevonden. Wel was de infectiedruk hier duidelijk hoger dan in het pootgoedperceel. Een barrier crop van 10 rijen tarwe verminderde de infectiedruk niet.De volgende conclusies kunnen worden getrokken. Aardappelopslag buiten en in aardappelvelden vormt een zeer belangrijke infectiebron. Zowel in 1976 als in 1977 vond de virusverspreiding plaats vóór de vlucht vanMyzus persicae begon, zoals deze werd bepaald met behulp van de gele Moericke vangbakken. Het effect van opzuiveren ten tijde van de virusverspreiding dient aan een nader onderzoek te worden onderworpen. De infectiedruk kan met de tabakstoets op meer directe wijze worden vastgesteld dan met bladluisvangsten mogelijk is.Wil men overgaan tot het vergelijken van de infectiedruk in verschillende gebieden of in verschillende jaren, dan dient de tabakstoets te worden gestandaardiseerd. Een voorstel hiertoe wordt gedaan.     

The virus-vector relationship of the tomato leafcurl virus (TLCV) and its vector,Bemisia tabaci gennadius (Hemiptera: aleyrodidae)

Basic studies carried out in India showed that the incubation period of TLCV in plants varied from 8 days in August to 90 days in winter. The acquisition threshold for the whitefly,Bemisia tabaci Gen., was 31 min; it resulted in 3% transmission. An acquisition access of 24 h for a female whitefly on a TLCV source resulted in 30% transmission. A minimum feeding period of 32 min was required by a viruliferous whitefly to cause infection on tomato test plants; this gave 4% transmission. With inoculation access of 24 h on tomato test plants, the transmission rose to 24%. Starving the vector for 1 h pre-acquisition or 1 h pre-inoculation resulted in higher levels of transmission of TLCV: 36 and 40%, respectively, compared with 20% for non-starved whiteflies. Extending the fasting period beyond 1 h resulted in a reduced transmission level. The whiteflies could acquire the virus from the cotyledonary leaves of an infected tomato plant, with a resultant 28% transmission; but infection did not occur when the whiteflies had an inoculation access to such leaves. Higher transmission rates were obtained when the younger leaves on tomato plants were used for acquisition and inoculation. Transmission was 8 and 38% when five whiteflies per plant were allowed 24 h of acquisition access to 11- and 2-month-old virus sources, respectively. After an acquisition access of 24 h to a TLCV source, male and female whiteflies retained their infectivity for 5 and 53 days, respectively. Nymphs can acquire and transmit the virus. When ten whiteflies of each sex were given 24 h of acquisition and of inoculation access, the subsequent transmission rate of males and females was 56 and 86%, respectively. This virus is not transovarially transmitted. Whitefly colonies raised on brinjal were more efficient (70 and 84% transmission in two experiments) than those raised on chilli, cotton, cowpea, tobacco or tomato.