Polymorphism of mitochondrial tRNA genes associated with the number of pigs born alive

Background: Mutations in mitochondrial tRNA genes have been widely reported association with human reproductions.It is also important to explore the effect on the number of piglets born alive(NBA).Here,1017 sows were used to investigate the association between polymorphisms in mitochondrial tRNA genes and NBA.Results: In total,16 mutations were found in mitochondrial tRNA genes,of which 13 mutations were significantly associated with NBA(P 0.05).The reproductions of mutant carriers were significantly greater than that of wild carriers by 0.989 piglets born alive/sow farrowing.To test whether the mutations altered the structure of mitochondrial tRNAs,the secondary and tertiary structures were predicted.In result,C2255 T changed the secondary structure of t RNA-Val by elongating the T stem and shrinking the T loop,and C2255 T and G2259 A in the tRNA-Val gene,C6217 T and T6219 C in the tRNA-Ala gene,and T15283 C in the tRNA-Glu gene altered the tertiary structure of their tRNAs,respectively by changing the folding form of the T arm,and C16487 T in the tRNA-Thr gene changed the tertiary structure of mitochondrial tRNA-Thr by influencing the folding form of the acceptor arm.Conclusions: Results highlight the effect of mitochondrial tRNA genes on the number of piglets born alive,and suggest that polymorphic sites of the tRNA genes be genetic markers for selection of pig reproduction.     

鸡鸭源性成分荧光LAMP检测方法的建立及应用

为快速鉴别牛羊肉中是否掺杂了鸡肉、鸭肉等其他肉类,基于Genie II等温扩增荧光检测系统,建立了检测鸡鸭源性成分的荧光LAMP检测方法。同时,优化了反应体系和条件,进行了特异性和灵敏度试验,以及市场检测应用。结果显示:鸡鸭源性成分荧光LAMP检测方法具有很强的特异性,除了相对应的动物源性成分外,其他13种动物源性成分均呈阴性;该方法检测鸡、鸭源性成分的灵敏度分别为0.88、3.32 pg/μL,均高于普通PCR 10倍;加入双加速引物,该方法的反应时间仅为15~20 min,10 min左右时就可进行实时初判;市场应用检测结果与普通PCR的符合率为100%。结果表明,本方法特异、灵敏,所需时间短,可用于鸡鸭源性成分的市场快速检测。     

The Anti‐Müllerian Hormone Profile is Linked with the In Vitro Embryo Production Capacity and Embryo Viability after Transfer but Cannot Predict Pregnancy Outcome

The current study investigated the possibility of using the AMH concentration as a predictor of the ability of Korean Hanwoo cows to produce cumulus‐oocyte complexes, embryos that survive after transfer as well as the pregnancy outcome of surrogates. Eight sessions of ovum pick‐up (OPU) were performed with 19 donor cows at an interval of 3–4 days. Antral follicle count (AFC), oocyte quality and in vitro embryo development were recorded for each cow. Embryos produced from cows with different AMH profiles were transferred into recipients (n = 96). Cows in the high (≥0.25 ng/ml) and intermediate (0.1≥ to <0.25 ng/ml) AMH groups had a significantly higher AFC per OPU session (20.40 ± 1.36 and 16.91 ± 1.52, respectively; mean ± standard deviation) than cows in the low AMH group (<0.1 ng/ml; 12.19 ± 2.14). In addition, more cumulus‐oocyte complexes per donor were recovered in the high (11.46 ± 1.22) and intermediate (7.38 ± 0.83) AMH groups than in the low AMH group (4.77 ± 0.44). The percentage of oocytes reached blastocyst stage was significantly higher in the intermediate (47.0%) and high (38.5%) AMH groups than in the low AMH group (32.3%). The number of embryos produced per cow was higher in the high (3.9 ± 0.2) and intermediate (6.9 ± 0.6) AMH groups than in the low AMH group (2.2 ± 0.3). The percentage of embryos that gave birth to viable calves when transferred into recipients was higher for those derived from cows in the intermediate AMH group (50.7%) than for those derived from cows in the low (35.7%) and high (36.4%) AMH groups. In conclusion, a single measurement of AMH concentration predicted the in vitro embryo production potential of donor Korean native cows before OPU and is linked with embryo viability after transfer into recipients.     

早熟棉花新品种金垦1261的选育

早熟陆地棉金垦1261生育前期长势稳健,综合农艺性状优良,高抗枯萎耐黄萎,丰产潜力大,适应性强。在辽宁省2年棉花区域试验中该品种比对照辽棉19号平均增产3.62%。本文概述了其选育过程、特征特性和栽培技术要点。     

微泡技术在有机物分离和提取中的应用

综述了微泡技术在分离、提取和降解有机物甲基叔丁基醚、多环芳烃、多氯联苯、活性淤泥、石油光泽几个方面的应用,与传统方法相比,微泡技术使甲基叔丁醚的利用率提高15倍;使多环芳烃和多氯联苯的去除时间缩短;使活性淤泥中较大固体减少缩短消除时间,并且增加能源生产;使含有有机酸的水溶液浊度从200NTU降低到2NTU,并且和表面光泽完全隔离。展望了微泡技术在色素或某类有机物的分离和提取中的应用,为色素或某类有机物的分离和提取提供更进一步的研究参考。     

碱性蛋白酶对大豆浓缩蛋白起泡性的影响

制取低成本、高蛋白含量的大豆浓缩蛋白时,乙醇产生变性作用,从而降低了大豆浓缩蛋白的功能特性,因此本研究采用碱性蛋白酶对醇法大豆浓缩蛋白进行改性.通过对酶浓度、底物浓度、改性时间与改性温度的单因素实验,针对起泡性进行研究,然后进行正交实验,最终得出碱性蛋白酶提高醇法大豆浓缩蛋白起泡性最佳工艺条件:酶用量(E/S)为6%、底物浓度为(W/V)8%、酶解时间为2h、酶解温度为50℃,在此条件下测得的起泡能力为113.3%,起泡稳定性为56.7%,分别提高到酶改性前的2.5、3.6倍.     

动物育种新方法在警犬育种领域中的应用前景

<正>警犬繁育工作既是警犬工作的基础,也是警犬工作的重点之一。动物育种从理论上讲,包括选种、选配、品系繁育和杂交改良,而在实际应用中,则根据动物的不同特点     

上蔟环境对茧质形成的影响研究——1.上蔟环境对蚕茧外观形质的影响

通过调查不同上蔟环境中所结蚕茧的性状，发现合理上蔟环境中所结的蚕茧茧质优；而不合理上蔟环境中所结的蚕茧次下茧多，且茧形小，茧色差、缩皱粗、乱、深，丝胶溶失率低，解舒差。     

犬的高原反应及应对策略探讨

<正>一、高原反应机理及主要生理表现高原反应是指到达一定海拔高度后,机体为适应因海拔高度而造成的低压、低氧、气候干燥寒冷、风速大、太阳辐射和紫外线照射量明显增大等变化而产生的自然生理反应,海拔高度一般达到2700米左右时,就会有高原反应。因组织细胞不能充分获得氧和利用氧,不能及时适应缺氧环境使生物氧化功能降低,使糖和脂肪不能     

呼伦贝尔盟航空森林消防工作探讨

介绍了海拉尔航空护林站的发展历史和现状。以海拉尔航空护林站的航空森林消防工作为例,阐述了呼伦贝尔盟航空森林消防工作坚持以科学发展现为指导,强化基础设施建设,争取资金投入,拓展航空护林业务等方面的发展思路,提出了今后一个时期发展的总体目标。