全文获取类型
收费全文 | 528篇 |
免费 | 43篇 |
国内免费 | 1篇 |
专业分类
林业 | 13篇 |
农学 | 16篇 |
基础科学 | 4篇 |
48篇 | |
综合类 | 104篇 |
农作物 | 18篇 |
水产渔业 | 11篇 |
畜牧兽医 | 307篇 |
园艺 | 14篇 |
植物保护 | 37篇 |
出版年
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 13篇 |
2017年 | 14篇 |
2016年 | 14篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 29篇 |
2012年 | 14篇 |
2011年 | 30篇 |
2010年 | 14篇 |
2009年 | 18篇 |
2008年 | 14篇 |
2007年 | 33篇 |
2006年 | 18篇 |
2005年 | 17篇 |
2004年 | 10篇 |
2003年 | 13篇 |
2002年 | 8篇 |
2001年 | 15篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 11篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 10篇 |
1991年 | 14篇 |
1990年 | 11篇 |
1989年 | 12篇 |
1988年 | 4篇 |
1987年 | 11篇 |
1986年 | 4篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1983年 | 11篇 |
1982年 | 5篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1975年 | 4篇 |
1974年 | 8篇 |
1973年 | 9篇 |
1972年 | 9篇 |
1970年 | 4篇 |
1967年 | 4篇 |
1926年 | 3篇 |
排序方式: 共有572条查询结果,搜索用时 31 毫秒
561.
"Dip-Pen" nanolithography 总被引:1,自引:0,他引:1
A direct-write "dip-pen" nanolithography (DPN) has been developed to deliver collections of molecules in a positive printing mode. An atomic force microscope (AFM) tip is used to write alkanethiols with 30-nanometer linewidth resolution on a gold thin film in a manner analogous to that of a dip pen. Molecules are delivered from the AFM tip to a solid substrate of interest via capillary transport, making DPN a potentially useful tool for creating and functionalizing nanoscale devices. 相似文献
562.
563.
Gilbert MT Tomsho LP Rendulic S Packard M Drautz DI Sher A Tikhonov A Dalén L Kuznetsova T Kosintsev P Campos PF Higham T Collins MJ Wilson AS Shidlovskiy F Buigues B Ericson PG Germonpré M Götherström A Iacumin P Nikolaev V Nowak-Kemp M Willerslev E Knight JR Irzyk GP Perbost CS Fredrikson KM Harkins TT Sheridan S Miller W Schuster SC 《Science (New York, N.Y.)》2007,317(5846):1927-1930
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections. 相似文献
564.
An early event in RNA interference (RNAi) is the cleavage of the initiating double-stranded RNA (dsRNA) to short pieces, 21 to 23 nucleotides in length. Here we describe a null mutation in dicer-1 (dcr-1), a gene proposed to encode the enzyme that generates these short RNAs. We find that dcr-1(-/-) animals have defects in RNAi under some, but not all, conditions. Mutant animals have germ line defects that lead to sterility, suggesting that cleavage of dsRNA to short pieces is a requisite event in normal development. 相似文献
565.
CA Guymon EN Hoggan NA Clark TP Rieker DM Walba CN Bowman 《Science (New York, N.Y.)》1997,275(5296):57-59
Photopolymerizable diacrylate monomers dissolved in fluid-layer smectic A and smectic C liquid crystal (LC) hosts exhibited significant spatial segregation and orientation that depend strongly on monomer structure. Small, flexible monomers such as 1,6-hexanediol diacrylate (HDDA) oriented parallel to the smectic layers and intercalated, whereas rod-shaped mesogen-like monomers such as 1,4-di-(4-(6-acryloyloxyhexyloxy)benzoyloxy)-2-methylbenzene (C6M) oriented normal to the smectic layers and collected within them. Such spatial segregation caused by the smectic layering dramatically enhanced photopolymerization rates; for HDDA, termination rates were reduced, whereas for C6M, both the termination and propagation rates were increased. These polymerization precursor structures suggest novel materials-design paradigms for gel LCs and nanophase-separated polymer systems. 相似文献
566.
567.
568.
569.
A new form of toxicity called equine fescue oedema is described. The clinical signs included inappetence, depression, and subcutaneous oedema of the head, neck, chest and abdomen. Affected horses had very low plasma albumin values. The toxicity affected 48 of 56 horses on six farms in different states of Australia, and 4 horses have died. All horses were grazing pastures that had been sown with varieties of Mediterranean tall fescue (Festuca arundinacea) that carry the endophyte known as Max P or Max Q. It is proposed that a pyrrolizidine alkaloid, N‐acetyl norloline, which is produced by the Max P endophyte, may be responsible for this new toxicity in horses. 相似文献
570.
DIDIER H. MATHON DVM PhD OLIVIER DOSSIN DVM PhD Diplomate ECVIM‐CA SOPHIE PALIERNE DVM PhD MATTHIEU CREMOUX DVM HERIBERTO RODRIGUEZ DVM PATRICIA MEYNAUD‐COLLARD DVM PhD JAN S. SUCHODOLSKI DVM PhD HERVE P. LEFEBVRE DVM PhD Diplomate ECVPT ANDRE AUTEFAGE DVM PhD Diplomate ECVS 《Veterinary surgery : VS》2009,38(8):967-974
Objective— To describe a laparoscopic‐sutured gastropexy technique in dogs and evaluate the tensile strength of the adhesion and effects on gastric function. Study Design— Experimental study. Animals— Female beagle dogs (n=7). Methods— A laparoscopic‐sutured gastropexy technique was evaluated by ex vivo tensile distraction tests 10 weeks after surgery. The effect of the adhesion on gastric emptying, mucosal permeability, and systemic inflammation were evaluated by monitoring the C‐reactive protein (CRP) and sucrose permeability, and by radiographic evaluation of gastric emptying 2 weeks before and 10 weeks after surgery. Results— Mean (±SD) tensile force to disrupt adhesions was 51.1±16.4 N. There was no significant postoperative increase in CRP concentration or change in sucrose permeability. The area under the curve representing the postprandial decrease in gastric radiographic area increased by 11% after gastropexy. Conclusions— This laparoscopic gastropexy technique had appropriate mechanical and functional characteristics with limited morbidity. Clinical Relevance— This laparoscopic‐sutured gastropexy provides adhesion strength comparable with other gastropexy techniques tested at 10 weeks postoperatively. Only minor changes in gastric emptying were observed 10 weeks after surgery. 相似文献