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121.
为掌握禾谷镰孢在甘肃中部地区的分布及变异情况,从根部表现有坏死和叶鞘发褐的小麦幼苗的不同部位、小麦地土壤及玉米籽粒、玉米茎秆上分离禾谷镰孢,并以形态学为基础,参照Nelson分类系统进行鉴定。结果表明,在分离到的43个镰刀菌菌株中,有14个菌株经鉴定为禾谷镰孢,均从玉米茎秆上分离到,小麦根部、小麦叶鞘、小麦地土壤、玉米籽粒中分离到的镰刀菌中未见禾谷镰孢。将禾谷镰孢在特定条件下培养后,发现14个禾谷镰孢菌株产生子囊壳的数量不同,为2~90个。在以Fg16为引物的PCR反应中,随机选取的11个禾谷镰孢菌株都产生0.41kb的PCR产物,而6个对照菌株都产生0.50kb的片段,证明引物Fg16可以区分禾谷镰孢菌株群体的遗传多态性。以Tri13为引物的PCR反应显示,11个禾谷镰孢菌株以及3个中国对照菌株都产生脱氧雪腐镰刀菌烯醇(DON)毒素,而3个澳大利亚对照菌株产生雪腐镰刀菌烯醇(NIV)毒素。 相似文献
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Steven R. James Lori Wing Ronald Knight Barbara Harrity Devid H. Lambert Bud Platt Al Reeves Bill Bohl Lind Sanford Alexander D. Pavlista Ed Plissey Richard Sawyer Joe Guenthner Willem Schrage Edward B. Radcilffe Nora Olsen Jeff Miller Rikki Sterrett Gale W. Harding Marcin Topoleweki 《American Journal of Potato Research》2000,77(6):369-390
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R J Williams F D Boudinot J A Smith A P Knight 《American journal of veterinary research》1990,51(3):371-375
Six mature Holstein bulls were given an 8-day course of phenylbutazone (PBZ) orally (loading dose, 12 mg of PBZ/kg of body weight and 7 maintenance doses of 6 mg of PBZ/kg, q 24 h). Plasma concentration-vs-time data were analyzed, using nonlinear regression modeling. The harmonic mean +/- pseudo-SD of the biologic half-life of PBZ was 61.8 +/- 12.8 hours. The arithmetic mean +/- SEM of the total body clearance and apparent volume of distribution were 0.0021 +/- 0.0001 L/h/kg and 0.201 +/- 0.009 L/kg, respectively. The predicted mean minimal plasma concentration of PBZ with this dosage regimen was 75.06 +/- 4.05 micrograms/ml. The predicted minimal plasma drug concentration was compared with the observed minimal plasma drug concentration in another group of bulls treated with PBZ for at least 60 days. Sixteen mature Holstein bulls were given approximately 6 mg of PBZ/kg, PO, daily for various musculoskeletal disorders. The mean observed minimal plasma concentration of PBZ in the 16 bulls was 76.10 +/- 2.04 micrograms/ml, whereas the mean predicted minimal plasma concentration was 74.69 +/- 3.10 micrograms/ml. Dosages of 4 to 6 mg of PBZ/kg, q 24 h, or 10 to 14 mg of PBZ/kg, q 48 h, provided therapeutic plasma concentrations of PBZ with minimal steady-state concentrations between 50 and 70 micrograms/ml. 相似文献
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K L Bryant E T Kornegay J W Knight K E Webb D R Notter 《Journal of animal science》1985,60(1):136-144
Three trials, utilizing a total of 240 crossbred gilts, were conducted to study the influence of 0 (NB) or 220 (SB) microgram supplemental biotin/kg of diet on feedlot performance, plasma biotin and development of toe lesions in developing gilts. Corn-soybean meal diets were fed from weaning to 92 kg body weight. Gilts were housed on expanded-metal floors to 50 kg body weight and on partially slatted concrete floors until completion of the trials. Feedlot performance, hair and structural soundness scores were not different (P greater than .10) between NB and SB gilts. Plasma biotin (PB) levels were elevated (P less than .01) when supplemental biotin was included in the diet. Gilts consuming SB diets had fewer (P less than .01) toe lesions/gilt and lower (P less than .01) toe lesion severity scores/gilt compared with gilts fed NB diets. Heel-horn erosion and heel cracks were the most frequent toe lesions observed. Fewer gilts (P less than .01) developed side-wall toe cracks when fed SB diets. Supplemental biotin reduced the frequency of individual toes containing heel-horn erosion (P less than .10), heel cracks (P less than .05) and side-wall toe cracks (P less than .05) with the severity of these lesions not affected (P greater than .10) by supplemental biotin. These results suggest that biotin levels in corn-soybean meal diets are adequate for feedlot performance, and that supplemental biotin can improve hoof integrity, but will not prevent toe lesions. 相似文献
129.
Sex determination of monomorphic birds by fibreoptic endoscopy 总被引:1,自引:0,他引:1
Fibreoptic endoscopy was performed in 1056 monomorphic birds of 144 different species belonging to 15 Orders. The purpose was to establish their sex by direct inspection of the gonads. The results suggest that although the technique involves surgical intervention it is generally more practical and accurate than chromosome analysis of lymphocytes or analysis of faecal steroids. Surgical anaesthesia is recommended on practical and humane grounds but this and the technique itself can be hazardous in old and fat birds, where a mortality rate of as high as 2 per cent was encountered. Cloacal examination of as high as 2 per cent was encountered. Cloacal examination is a more practical technique for sex determination in penguins. 相似文献
130.
The acute toxicity for sheep of 3 alkaloids that occur in Phalaris acquatica was examined by intravenous and oral administration. The lowest tested dose rates that produced clinically observed signs were, for 5-methoxy dimethyltryptamine, 0.1 mg/kg body weight intravenously and 40 mg/kg orally; for gramine, 10 mg/kg intravenously and 500 mg/kg orally; and for hordenine, 20 mg/kg intravenously and 800 mg/kg orally. All induced the clinical signs observed in the nervous form of phalaris toxicity, but none induced the cardiac, sudden death, syndrome. 相似文献