Fungicide resistance status and chemical control options for the brassica pathogen Pyrenopeziza brassicae

Pyrenopeziza brassicae causes leaf spot disease of Brassicaceae in Europe/Oceania (lineage 1) and North America (lineage 2). In Europe, fungicides currently used for disease management are sterol 14α-demethylase (CYP51) inhibitors (azoles), quinone outside inhibitors (QoIs), and succinate dehydrogenase inhibitors (SDHIs); methyl benzimidazole carbamates (MBCs) are no longer applied. In this study, in vitro screening revealed European populations (collected 2018–2020) had shifted towards decreased azole sensitivity, but the North American population (2014–2016) was highly sensitive. Genotyping revealed CYP51 substitutions G460S or S508T were prevalent in European populations, often with a CYP51 promoter insert. Compared to wildtype CYP51 isolates, those with G460S plus an insert (44/46/151/210/302 bp) were c.25–32-fold and c.50-fold less sensitive to tebuconazole and prochloraz, respectively; those with S508T plus an insert (44/46/151/233 bp) were c.9–15-fold and c.25–40-fold less sensitive to tebuconazole and prochloraz, respectively. Selection for G460S (quantified via pyrosequencing) under different fungicide regimes was investigated in UK field trials, but G460S levels were high (c.76%) before treatment, so further selection during the trials was unclear. Despite the high G460S frequency and low disease pressure, yield data indicated measurable benefit for both azole- and non-azole-based programmes. In vitro screening against the MBC carbendazim showed European populations were predominantly moderately resistant/resistant; the North American population was sensitive. European and North American populations were sensitive to QoI (pyraclostrobin) and SDHI (penthiopyrad) fungicides. Results support an azole plus QoI/SDHI mixing partner for robust disease control and decreased risk of resistance, with continued sensitivity monitoring to ensure optimal strategies are deployed.     

Isoleucine epimerization for dating marine sediments: importance of analyzing monospecific foraminiferal samples

The rate of isoleucine epimerization in fossil planktonic foraminifera is strongly species-dependent. Alloisoleucine/isoleucine ratios of two species of the same age can vary by more than a factor of 2. This finding, in combination with the known temporal and spatial variability of foraminiferal assemblages, demonstrates the critical importance of basing geochronological studies of marine sediments on monospecific samples. One rapidly epimerizing species generates a calibration curve of potentially high precision for dating sediments between the ages of about 50,000 to 400,000 years.     

Morphological development in Brassica oleracea is modulated by in vivo treatment with 5-azacytidine

Summary

Treatment with the demethylating agent 5-azacytidine induced a range of developmental changes at high frequency in Brassica oleracea vars. botrytis, italic and alboglabra. The spectrum of morphological variation was similar to that previously observed in Brassica, either as somaclonal variation as a result of tissue culture, or as a result of mutation with nitroso-N-methylurea. Where floral development permitted, inheritance of developmental variants was demonstrated to the M₂ generation. The role of methylation in determining the development and apparent genetic diversity within Brassica oleracea is discussed.     

Venus winds are zonal and retrograde below the clouds

Winds in the lower atmosphere of Venus, inferred from three-dimensional radio interferometric tracking of the descents of the Pioneer day and north probes, are predominantly easterly with speeds of about 1 meter per second near the surface, 50 meters per second at the bottom of the clouds, and more than 200 meters per second within the densest, middle cloud layer. Between about 25 and 55 kilometers altitude the average flow was slanted equatorward, with superimposed wavelike motions and alternating layers of high and low shear.     

New insight into the genetic structure of the Allolobophora chlorotica aggregate in Europe using microsatellite and mitochondrial data

The taxonomic status of Allolobophora chlorotica is still the subject of considerable discussion. After the recent experimental demonstration that the two regularly observed colour morphs (pink and green) were not fully interfertile and likely represent two distinct species, molecular analyses revealed a more complex picture with the occurrence of five mitochondrial lineages (named L1 to L5) in A. chlorotica, two within the green morph and three within the pink morph. Although nuclear markers (AFLPs) confirmed that the pink morph might consist of three different taxa, AFLPs data suggested that the green morph may be a single taxon. In order to further characterize the population genetic structure within A. chlorotica in addition to test for reproductive isolation between lineages, eight polymorphic microsatellite loci were developed using a microsatellite-enriched genomic library from an individual belonging to lineage L2. The number of alleles per locus varied from 9 to 29 in a set of individuals belonging to L2. Considerable sub-structure was observed within L2 populations suggesting a low dispersal capability in this species and a distribution of the individuals in patches that could function as panmictic units. These markers were transferable to the other lineages but only five loci could be amplified consistently in four of the lineages (L1, L2, L3 and L4). Microsatellite data confirmed that the green morph represents a single taxon. Although the integrity of the three previously documented lineages within the pink morph was also generally supported by the data, microsatellites provided evidence for hybridization between lineages and between morphs in the field. Moreover, mitochondrial data revealed the existence of two additional mitochondrial lineages within the pink morph. The taxonomic status of the pink morph remains thus unclear, requiring a thorough and comprehensive study.