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221.
Ruijuan Li Jinfeng Zhao Chuanfan Sun Wenjing Lu Chengjin Guo Kai Xiao 《Frontiers of Agriculture in China》2010,4(2):195-209
As a kind of enzyme widely existing in eukaryotic species, especially in grains and oil seeds, phytases play an important
role in the degradation of some phosphates containing organic molecules. So far, phytases derived from various species have
been successfully used as animal feed additives. It has also been experimentally verified that phytases have a potential use
in generating crop germplasm with high phosphorus use efficiency, based on their biochemical role in releasing Pi from the
phytate and its derivatives. In this paper, the biochemical properties, molecular characterizations, functions and the potential
application perspective of phytases are reviewed and commented on, aiming at the further exploration of the biochemical and
molecular characterizations, and promotion of the application of phytases, a kind of important enzyme possessing potential
use in animal feeding and creation of high P use crop cultivars, in the future. 相似文献
222.
223.
Xing-Pu Li Su-Que Lan Ye-Lun Zhang Yu-Ping Liu 《Genetic Resources and Crop Evolution》2010,57(7):1007-1012
Pericarp color in wheat is an important trait related to nutrients and health benefits. Identification of molecular markers
closely linked to the grain color genes will help utilize pericarp trait in wheat breeding. Genetic studies involving F1, F2, and F3 populations from the cross Jizi 439 (purple grain)/Gao8901 (white grain) showed that the purple grain color was controlled
by two genes. By using the bulked segregant analysis (BSA) approach, two DNA pools were established from the dark purple-grain
and white-grain plants respectively. Among the 230 pairs of microsatellite primers used in this study, two markers showed
polymorphism in DNA pools, parents, F1 and F2 populations. Linkage analysis indicated that the two markers Xgwm47 and Xgwm155 were linked with two purple pigment genes. One of the purple pigment genes is located on chromosome 2AS and is 34.7cM away
from the marker Xgwm47, whereas another gene is mapped on 3AL with 14.7cM away from marker Xgwm155. 相似文献
224.
225.
Living in poverty is associated with high levels of protracted stress associated with health problems. Economic and food insecurity
are particularly poignant aspects of poverty and condition the work of securing basic daily needs of families. Recent studies
suggest that levels of stress increase as family food needs rise. This paper presents new findings which clarify the relationship
of food provisioning to stress levels, by examining actual food provisioning strategies and food insecurity among the Northern
Cheyenne Indians of southeastern Montana. Results clearly show that stress varies by types of food acquisition strategies.
Contrary to our expectations, more complex strategies, including relatively unpredictable and cumbersome food provisioning
activities, are not linked to higher stress levels in our analysis. Controlling for food security levels, households using
a combination of local programs and informal subsistence sources are the least stressed, despite the demands of managing a
large number of food sources. Households primarily using Food Stamps are the most likely to experience high levels of stress.
Interviews with Food Stamp recipients show that potential sources of stress include inadequate allocations of Food Stamps,
difficulty achieving and maintaining eligibility, challenges to complying with paperwork and appointment requirements, as
well as personal obstacles and community barriers to making food stamps last. Analyses indicate that contradictions between
local cultural norms for food provisioning and the realities of food insecurity in this context promote strategies emphasizing
greater independence from federal food programs. 相似文献
226.
Changsheng Chen Chaotian Xie Dehua Ji Yan Liang Lingmin Zhao 《Aquaculture International》2010,18(6):1045-1060
To select a reliable and sensitive method for discriminating strains of Porphyra haitanensis, the nucleotide sequence of the internal transcribed spacer 1 to internal transcribed spacer 2 regions (ITS-5.8S) of nuclear
ribosomal DNA and the intergenic spacer region of RUBISCO were compared in five wild and five cultivated Porphyra haitanensis strains. Based on molecular analyses, sequences of ITS-5.8S (about 1,210 bp) could be divided into three regions: ITS1, 5.8S,
and ITS2. The ITS1 and ITS2 sequences of each strain differed, even between individuals collected from the same site. In contrast,
5.8S rDNA and RUBISCO spacer sequences were identical among the ten P. haitanensis strains, although differences were found among different Porphyra species. Phylogenetic analysis also supported these conclusions. These sequence features of highly conserved regions and
diversified regions that occurred repeatedly in ITS-5.8S could be useful in discriminating germplasm of P. haitanensis strains or Porphyra species. In contrast, the RUBISCO spacer is only suitable for identifying Porphyra species. New coupled primers were designed to amplify only the 5.8S rDNA and ITS2 region of Porphyra. The sequences of these amplified fragments can be readily used to identify germplasm or to perform phylogenetic analysis
of Porphyra spp. 相似文献
227.
A study was conducted to investigate the effects of skid trail slope and traffic levels on soil disturbances at two soil depths
(0–10 and 10–20 cm). The treatments were set at four traffic levels (2, 7, 12 and 20), two slope classes (<20% and >20%) and
two soil depths (0–10 and 10–20 cm). Results show that skidder traffic, longitudinal slope and soil depth have significant
effect on soil bulk density in skid trail. Comparison of average soil bulk density in different traffic levels shows that
there are significant differences in average bulk density between different traffic levels and control (p<0.05). The average bulk densities in different slopes and soil depths are significantly increased with increase in traffic
levels, maximized at 12 passes (p<0.05), but there are no significant differences between 12 and 20 passes. The interaction effects between traffic and soil
depth are significant (F
0.05,3=0.109, p<0.001). For all traffic treatments, there are significant differences in soil moisture content between the two slope classes
and the two depths (p<0.001). However, the interaction effects between traffic levels and slope classes are not significant (p >0.05), although skidder traffic and slope affected soil moisture content. 相似文献
228.
Yongju Zhao Jiahua Zhang Hong Wei Xinming Sun Biao Mu Mingju Yu Lingbing Wang 《Tropical animal health and production》2010,42(6):1257-1262
Two experiments were undertaken to select the efficient method applied for goat estrous synchronization. In experiment 1,
a total of 120 does (Capra hircus) were divided into five groups with a randomized block design, and the does of treatment 1 were synchronized by intravaginal
sponges impregnated with 30 mg Levonorgestrel inserted for 10 days. Does of treatments 2, 3, and 4 were treated with further
injection of 25 IU follicle-stimulating hormone (FSH), 0.05 mg prostaglandin F2 alpha (PGF2α), and 25 IU FSH + 0.05 mg PGF2α at sponge withdrawal, respectively. The does in the control group (n = 40) without estrous synchronization treatment and natural estrous does were observed. In experiment 2, a total of 140 does
in five goat farms in breeding and non-breeding seasons were treated with the selective efficient procedure. The results presented
that all the employed treatments were capable of inducing and synchronizing estrous goats. According to estrous response and
economy, the use of intravaginal sponges impregnated with 30 mg Levonorgestrel and 0.05 mg PGF2α (treatment 3) is the first choice for estrous synchronization, and 95.0% of synchronized does demonstrated estrus, which
was significantly higher than that of treatment 1 (P < 0.05) and control group (P < 0.01). The percentages of ovulating of treatments 3 and 4 were the same (95.0%), which were significantly higher than that
of treatment 1 (P < 0.01). The ovulation rates among different groups were not significant (P > 0.05). When the selective procedure was applied to five goat farms, 85.7% (120/140) of does demonstrated estrus, and the
kidding percentage, litter size, and prolificacy rate were 53.6%, 0.95, and 177%, respectively. 相似文献
229.
George Rigos Alexandros Samartzis Morgane Henry Eleni Fountoulaki Efthimia Cotou John Sweetman Simon Davies Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104
The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead
sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet).
Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final
weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells,
haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst
activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue
distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200
ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly
affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory
burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300
ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication
that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point
of view. 相似文献
230.