Feline diabetes mellitus in the UK: the prevalence within an insured cat population and a questionnaire-based putative risk factor analysis

Prevalence and risk factors for the development of diabetes mellitus (DM) in cats in the United Kingdom have not previously been reported. The prevalence of DM was evaluated in a large insured population and was found to be 1 in 230 cats. In this insured cat population Burmese cats were 3.7 times more likely to develop DM than non-pedigree cats. A convenience-sampling questionnaire-based study was used in order to identify putative risk factors for the development of DM. The univariate risk factor analysis identified being male, neutered, inactive, weighing >or=5 kg and having a history of corticosteroid treatment as significant risk factors for the development of DM in these cats. In addition, male cats treated with megestrol acetate had a significantly increased risk of developing DM compared to females. In contrast, there was no difference in DM occurrence between male and female Burmese cats. A multivariate classification tree-based model on the questionnaire data looking for interactions between risk factors, identified gender as the most important overall risk factor for the development of DM with low physical activity being the next most important risk factor for female cats and breed the next most important for male cats.     

Placement of jejunal feeding tubes for post-gastric feeding

In veterinary patients, postgastric feeding is indicated for uncontrollable gastric vomiting, gastroparesis, biliary tract disease, pancreatitis, and for patients at increased risk for aspirating secondary to decreased mentation, prolonged recumbency, or an unprotected airway. Postgastric feeding may be implemented via the placement of a jejunal feeding tube. These tubes can be placed surgically (jejunostomy tubes) or with fluoroscopic or endoscopic guidance. This article will focus on methods of jejunal feeding-tube placement, advantages and disadvantages of the methods described, and complications associated with jejunal feeding.     

Classification of smoke tainted wines using mid-infrared spectroscopy and chemometrics

In this study, the suitability of mid-infrared (MIR) spectroscopy, combined with principal component analysis (PCA) and linear discriminant analysis (LDA), was evaluated as a rapid analytical technique to identify smoke tainted wines. Control (i.e., unsmoked) and smoke-affected wines (260 in total) from experimental and commercial sources were analyzed by MIR spectroscopy and chemometrics. The concentrations of guaiacol and 4-methylguaiacol were also determined using gas chromatography-mass spectrometry (GC-MS), as markers of smoke taint. LDA models correctly classified 61% of control wines and 70% of smoke-affected wines. Classification rates were found to be influenced by the extent of smoke taint (based on GC-MS and informal sensory assessment), as well as qualitative differences in wine composition due to grape variety and oak maturation. Overall, the potential application of MIR spectroscopy combined with chemometrics as a rapid analytical technique for screening smoke-affected wines was demonstrated.     

Emergence of four weed species in response to rainfall and temperature

The key to achieving effective weed control is to target them when they are young. Understanding their temperature and moisture requirements for emergence will help growers to develop timely control strategies. This study examined the emergence requirements of four key species: Conyza bonariensis, Sonchus oleraceus, Echinochloa colona and Chloris virgata. Two experiments were conducted in a growth room with temperatures of 25/15 and 30/20°C day/night with a photoperiod of 12 h. The first experiment examined emergence under single rainfall events of 0, 2, 5, 10, 20, 30 and 50 mm. The second experiment examined emergence under accumulated rainfall events of 0 mm, 5 mm × 2 (consecutive) days, 5 mm × 4 days, 5 mm × 6 days, 10 mm × 1 day, 10 mm × 2 days and 10 mm × 3 days. The temperature was not significant on emergence in both experiments. In the first experiment, at least 10 mm of rainfall was required for the emergence of all the species, with significantly higher germination rates at rainfall amounts of >20 mm. In the second experiment, the species had higher emergence rates with accumulated rainfall over consecutive days, rather than single rainfall events of the same amount. The length of time that the soil surface remained above the permanent wilting point, allowing the imbibition of water, was also an important factor. This study has provided information on the drivers for emergence of these species and will assist growers in determining what rainfall events are likely to trigger emergence in the field and make timely weed management decisions.     

Nematophagous Verticillium spp. in soils infested with Meloidogyne spp. in Cuba: Isolation and screening

Root-knot nematodes cause substantial economic loss of yield in coffee plantations and vegetable crops in Cuba. At present, methods to control the nematodes are ineffective or inappropriate and alternatives are being sought. The nematophagous fungus Verticillium chlamydosporium (Goddard) was isolated from soils collected from coffee plantations and infected root-knot nematode eggs from roots of tomato plants grown in these soils. A total of 83 isolates were collected and identified morphologically as V. chlamydosporium var. chlamydosporium, V. chlamydosporium var. catenulatum, V. psalliotae, V. suchlasporium and an isolate of V. chlamydosporium var. chlamydosporium with unusually large dictyochlamydospores. From these, 24 that represented a range of origins were selected and screened for their ability to parasitize eggs of root-knot nematodes, colonize the rhizosphere of barley roots and produce chlamydospores. None of the isolates grew at temperatures below 15°C and V. suchlasporium grew at a faster rate at lower temperatures than the other isolates. These were also screened in the glasshouse and V. chlamydosporium var. catenulatum caused the greatest reduction in nematode populations. One isolate of each subspecies of V. chlamydosporium was tested with the standard, Rothamsted isolate 10, on a range of host plants. The greatest reduction in numbers of nematodes occurred on tomato plants (cv. Pixie). The Rothamsted isolate 10 reduced numbers of nematodes toa greater extent than the other isolates, and therefore has the greatest potential as a biological control agent of root-knot nematodes.     

Identification of natural oak lactone precursors in extracts of american and French oak woods by liquid chromatography-tandem mass spectrometry

A method for the screening of potential natural oak lactone precursors in oak wood extracts using LC-MS/MS combined with information-dependent acquisition was developed. The method was applied to extracts of American and French oak woods. As a result, cis-3-methyl-4-galloyloxyoctanoic acid (ring-opened cis-oak lactone gallate), (3S,4S)- and (3S,4R)-3-methyl-4-O-beta-D-glucopyranosyloctanoic acid (ring-opened cis- and trans-oak lactone glucoside), and (3S,4S)-3-methyl-4-O-(6'-O-galloyl)-beta-D-glucopyranosyloctanoic acid (ring-opened cis-oak lactone galloylglucoside) were identified as natural oak lactone precursors in the extracts by comparison with the respective synthetic reference compounds. In addition, the ring-opened oak lactone rutinoside was tentatively identified in the extracts. Three apparent isomers of the ring-opened cis-oak lactone galloylglucoside were also observed.     

Visualization of Channels and Cavities of Corn and Sorghum Starch Granules

Evidence is presented that corn (maize) and sorghum starch granules have channels that connect a central cavity to the external environment. A mutant sorghum starch with numerous, large surface pores was treated with a methanolic solution of merbromin and sectioned. Light, fluorescence, and compositional backscattering electron microscopy revealed channels connecting an internal cavity to the external surface in most granules. Cavities and channels could also be seen in whole corn and sorghum starch granules treated with merbromin in methanol and viewed by fluorescence microscopy. Treatment of sorghum starch granules with an aqueous solution of merbromin revealed that the molecule penetrated the granule matrix under even slightly swelling conditions. Light microscopy showed cavities in unstained, whole, dry corn and sorghum starch granules mounted in immersion oil.     

Milk carbohydrates of the echidna and the platypus

The principal neutral carbohydrate of three samples of echidna milk was identified as a trisaccharide, fucosyllactose. That of a sample of platypus milk was a tetrasaccharide, difucosyllactose. Free lactose was found in small amounts only. The milk carbohydrate of monotremes is distinguished from that of both marsupials and placental mammals by its high fucose content.     

The Fate of Polymeric Quaternary Ammonium Salts from Cosmetics in Wastewater Treatment Plants

Polymeric quaternary ammonium salts or polyquaterniums used in cosmetics have been categorised as chemicals of concern in wastewater treatment plant (WWTP) effluent largely on the basis of emerging evidence of toxicity to aquatic organisms. However, little is known of their environmental fate and behaviour due to analytical difficulties with sample matrices. Their properties of negligible volatilisation and biotransformation enable the common fugacity-based model for WWTPs to be simplified to an equifugacity one where a compound has the same fugacity regardless of phase or position in the plant??s process train. To gain an appreciation of their fate, this approach is used to calculate removal efficiencies in WWTPs. These can be determined without calculating phase-specific fugacity capacity constants. To predict effluent concentrations however, an aquivalence approach is necessary because of the lack of volatility of these compounds. Using previously measured biosolids/water distribution coefficients for common polyquaterniums found in cosmetics and flow rate data from a local municipal WWTP in South East Queensland, Australia, the removal efficiencies of the polyquaterniums of interest are predicted to be only 25% or less, meaning relatively little attenuation in the WWTP. A Monte Carlo simulation shows a roughly normal distribution in the model output of polyquaternium removal efficiency, with a mean and mode of approximately 26%. A sensitivity analysis confirms that the model output is most sensitive to the magnitude of the biosolids/water distribution coefficient compound and shows WWTP data such as biosolids removal efficiency have only a relatively small effect.