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51.
L J Nu?ez-Vergara J A Squella E M Gonzalez 《Journal of the Association of Official Analytical Chemists》1979,62(3):556-559
A polarographic method has been developed for the quantitative analysis of cephradine and its dosage forms. Direct determinations on capsules are carried out; excipients and coloring matter do not interfere in the determination. The electroactive product is formed by acidic hydrolysis with 5.0N HCl and heating at 80 degrees C for 60 min. Two polarographic waves are obtained: I = -0.46 V and II = -0.78 v vs. SCE. Both reduction waves are diffusion controlled. Wave I is preferred for analytical purposes. The precise chemical identity of the electroactive product has not been determined, but UV spectral data and the TLC Rf value are reported. A linear relation is established for levels of cephradine between 10(-2) and 10(-5)M in 5.0N HCl. 相似文献
52.
Seven types of fungal melanins were compared with humic compounds from chernozem and podzol soils and with commercial humic acid. The results indicate some similarities but also distinct differences between the melanins and humic substances in spectral characteristics in the UV and visible regions and in their resistance to thermal degradation. 相似文献
53.
Helvécio De-Polli Eiichi Matsui Johanna D?bereiner Eneas Salati 《Soil biology & biochemistry》1977,9(2):119-123
Intact soil cores containing plants of Paspalum notatum or Digitaria decumbens were selected with the acetylene reduction method, and then exposed to 15N15 to confirm nitrogen fixation in tropical grass-bacteria associations. In a preliminary experiment with P. notatum15N2 incorporation was slow but progressive during 24 h in roots but translocation to rhizomes and leaves ceased after 17h. With improved assay chambers, enrichments of 0.151 and 0.563 15N atom % excess were obtained in roots of D. decumbens cv transvala and P. notatum systems respectively, after 3 days. Enrichments in rhizomes were similar to those of roots; however in the leaves only 8% of root enrichment was observed. The addition of sucrose to the soil doubled N2-fixation in roots in both grass species studied, but did not result in increased incorporation into the leaves of P. notatum. 相似文献
54.
Torés Juan Antonio González María Dolores Pozo Eva Urdiales José Luis Medina Miguel Ángel Sánchez-Jiménez Francisca 《Phytoparasitica》1999,27(3):207-213
Chlorpheniramine (CPA) is an antihistaminic that changes the conformation of DNA and inhibits polyamine biosynthesis in mammalian
cells. In the present work, we tested the effect of CPA on four genera of fungi species (Altemaria alternata, Botrytis cinerea, Cladosporium cladosporioides and threePenicillium spp.) grownin vitro. Similar growth inhibitions of these genera were produced by 0.5 raM iprodione, CPA and histidinol, but CPA was the most effective.
The CPA sensitivities of the twoB. cinerea strains were different. Putrescine did not restore the fungal growth inhibited by CPA. 相似文献
55.
Carlos Garrido María Carbú Francisco Javier Fernández-Acero Giles Budge Inmaculada Vallejo Alison Colyer Jesús M. Cantoral 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(4):409-415
Anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry, Fragaria × ananassa. This study identifies the Colletotrichum spp. which causes strawberry anthracnose in the southwest of Spain. A survey of the region was carried out, and the strains
isolated were identified as C. acutatum by using the polymerase chain reaction (PCR) with genus and species-specific primers, demonstrating that this species is
currently the causal agent of strawberry anthracnose in the studied region. The pathogenicity of C. acutatum and C. gloeosporioides strains was evaluated on two principal strawberry cultivars (cvs Camarosa and Ventana) under field conditions, the latter
being more pathogenic than the former. 相似文献
56.
Andrés F. Sánchez-Restrepo Nadia L. Jiménez Viviana A. Confalonieri Luis A. Calcaterra 《国际虫害防治杂志》2019,65(3):244-257
AbstractLeaf-cutting ants (LCA) are considered one of the main herbivores and one of the most destructive pest insects of the Neotropics. Northeastern Argentina harbors the greatest species richness of these ants and in turn comprises the highest surface with forest plantations. Our aim was to establish which species of leaf-cutting ants are most commonly associated with forest plantations by analyzing their geographic distribution using published and unpublished species occurrence data. Also, estimate their potential areas of distribution along a latitudinal gradient that entirely encompasses northeastern Argentina using Ecological Niche Modeling. Only seven of the 20 species recorded were strongly associated with productive systems along the gradient, but only 2–3 species in each region could be considered high-risk species for forest plantations. High-risk species composition shows a turnover between regions. Our models show the potential distribution areas where LCA could become more abundant and dominant, and possibly causing a detrimental effect on the forest plantations in the studied region. We find that ecological niche models are useful tools to assess the environmental suitability of important LCA. 相似文献
57.
George?J.?VandemarkEmail author Niklaus?J.?Grünwald 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(4):309-316
Aphanomyces euteiches causes severe root rot of peas. Resistance is limited in commercial pea cultivars. Real-time fluorescent PCR assay specific for A. euteiches was used to study the relationship between disease severity and pathogen DNA content in infected peas. Five pea genotypes ranging in levels of resistance were inoculated with five isolates of A. euteiches. Plants were visually rated for disease development and the amount of pathogen DNA in roots was determined using the PCR assay. The susceptible genotypes Genie, DSP and Bolero tended to have significantly more disease and more pathogen DNA than the resistant genotypes 90-2079 and PI 180693. PI 180693 consistently had less disease, while 90-2079 had the lowest amount of pathogen DNA. The Spearman correlation between pathogen DNA quantity and disease development was positive and significant (P < 0.05) for three isolates, but was not significant for two other isolates. This suggests that the real-time PCR assay may have limited application as a selection tool for resistance in pea to A. euteiches. Its utility as a selection tool would be dependent on the correlation between disease development and pathogen DNA content for a given pathogen isolate. The accuracy and specificity of the real-time PCR assay suggests considerable application for the assay in the study of mechanisms of disease resistance and the study of microbial population dynamics in plants. 相似文献
58.
Karel?PetrzikEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(4):355-360
Apple mosaic virus (ApMV, genus Ilarvirus) was detected in pears, a previously non-reported virus host. No symptoms were visible on the hosts leaves. Seventeen out of 22 randomly selected pear trees in Italy (Lombardy) and in three regions in the Czech Republic were ApMV-infected. All nine newly sequenced ApMV isolates from pears had a 15-nucleotide insertion in the capsid protein gene in identical position of that of apple isolates compared with isolates from hop and prunes. The insertion is the most prominent (but not essential) modification of the capsid protein gene, which results in a phylogenetic separation of ApMV isolates into three clusters. Sequence analysis data of an additional 15 isolates revealed a sequence correlation with kernelled fruit trees (apple and pear). 相似文献
59.
Zaida Mu?oz Assumpció Moret Sandra Garcés 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(4):331-337
The pathogenic fungi Verticillium alboatrum and Diplodia scrobiculata were assayed for biological control of Diplodia pinea on Aleppo pine (Pinus halepensis) in Catalonia (north-eastern Spain). Young shoots were pre-treated with inoculations of either V. dahliae or D. scrobiculata, by placing colonized agar plugs on wounds made by removing a single needle fascicle. An inoculation with D. pinea was performed 15 days later. Two months after the shoot inoculations, the canker length on the stems was measured and the
percentage of shoot dieback calculated. Verticillium dahliae and D. scrobiculata were found to significantly reduce the canker length of D. pinea (P < 0.05) when compared with positive controls. Diplodia pinea was slightly more sensitive to V. dahliae than to D. scrobiculata, but no significant differences (P > 0.05) were observed in the mean canker length between the two treatments. Trees pre-inoculated with V. dahliae resulted in 31.12% shoot dieback, while those pre-inoculated with D. scrobiculata resulted in 32.18% shoot dieback, compared with positive controls (42.85%). 相似文献
60.