Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room

In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue samples which were kept in 4℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.     

对水坝防渗加固技术的探讨

水坝的防渗加固问题多年以来就一直是水利工程施工的一个重要方面。贵州省余庆县松烟镇地处长江、珠江上游,因此为了有效保障当地人们和长江、珠江中下游地区的安全和利益。对该地水坝的防渗加固问题要尤为重视,对水坝防渗加固的技术问题进行探讨。     

直播稻田杂草的生态特点和控制技术研究

近年来,随着直播稻种植面积不断扩大和栽培技术不断改革,田间草相也随生态条件的不同而发生了变化。水稻前期干、湿栽培,十分有利老草复活和新草的萌发生长。直播稻田杂草危害已成为阻碍直播稻优质高产和大面积推广应用的主要障碍。为了明确直播稻田杂草的生态特点和杂草萌发规律,科学制定防除策略,积极探索直播稻田杂草的化除技术,安全、有效、经济地将杂草危害损失控制在最低限度,我们于2003年开展了直播稻田杂草的生态特点和防除技术研究,初步摸清直播稻田出草规律,得出化除配套技术,并进行了大面积推广应用,取得了显著的灭草、增产效果…     

Expression of urotensinⅡ receptor GPR14 in aorta of apoE knockout mice

AIM: To investigate the expression of the urotensin Ⅱ (UⅡ) receptor GPR14 in the aorta of apoE knockout mouse. METHODS: The expression of GPR14 in the aorta of apoE knockout C57BL/6J mice at various ages (18 weeks, 28 weeks, and 38 weeks old, respectively) was determined with competitive RT-PCR. A binding assay of ［125I］-UⅡ on the aortic tissue was also performed in 28 weeks group. RESULTS: We found significant upregulation of GPR14 mRNA at all three ages. Compared with wild type group at the same age, the GPR14 mRNA level in apoE knockout mice increased 54.2% in 18 week group (P<0.05), 50.0% in 28 weeks group (P<0.05) and 97.0% in 38 weeks group (P<0.01). In the knockout group or in the wild type group, expressions of GPR14 in the 28 weeks time point were significantly higher than that in other two age groups, and there was no difference between the 18 weeks and 38 weeks group. In the binding assay, the Bmax of ［125I］-UⅡ to the aorta of apoE knockout mouse at 28 weeks increased 64% compared with the wild type (P<0.01), and no difference about the Kd between the two groups was observed. CONCLUSION: UⅡ and its receptor probably play an important role in the development of atherosclerosis.     

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.     

小麦禾谷孢囊线虫寄生范围的研究

经测试表明,禾谷孢囊线虫(Heterodera avenae)对长江中游地区栽种的小麦、裸大麦、米大麦、家燕麦、野燕麦、黑麦草、鹅冠草、苇状羊茅、球茎草芦和鸭茅均能侵染,这些植物是该线虫的良好寄主,但感染率差异不显著。该线虫能侵染玉米,但很难完成生活史,不侵染供试的红三叶草和紫苜蓿2种非禾本科牧草。在不同的寄主根内,线虫发育速度有差异,单位重量根内虫口数亦有异,黑麦草内最多,鲜根有线虫4.45条/g,鸭茅最少为1.26条/g。     

感染烟草花叶病毒(TMV)的烟叶在显症前的荧光光谱变化

通过测试ＴＭＶ侵染叶片的叶绿素含量与荧光光谱特征的动态变化，分析影响荧光光谱的因素，在以４８０ｎｍ作为激发波长时，叶片６８５ｎｍ与７４０ｎｍ荧光强度之比（Ｆ６８５／Ｆ７４０）随着病毒侵染时间的延长而增加，叶绿素含量则随着病毒侵染时间的延长而降低，因此提出可用Ｆ６８５／Ｆ７４０与叶绿素含量之比作为ＴＭＶ早期诊断的指标。     

天水毛白杨种源试验的研究

本文介绍了毛白杨８个种源、２２０个无性系，在甘肃天水地区开展的种源选择试验及优良无性系筛选结果。经过７年的研究，摸清了毛白杨的变异规律及模式，筛选出３个种源，３０个优良无性系，遗传增益达２２％～２８％，为造林区别提供了科学依据。试验证明无性系有种是一种投资少，见效快，经济效益高的良种选有方法，可降低成本５～６倍。     

湖南水稻白翅叶蝉的研究

白翅叶蝉Erythroneura subrufa(Motschulsky)成虫与若虫均以刺吸口器刺进水稻叶片组织内吸取叶汁和叶绿素,被害初期,叶片上出现零星小白点,随后出现长短不一的点状白色斑条,受害严重的稻叶上满布白色斑纹,叶绿素丧失,叶片干萎。在稻叶蝉中除一点小叶蝉被害状出现小白点外,其他叶蝉为害水稻时,并不直接破坏叶绿素,亦不呈现白色斑纹,而是直接吸食叶液,使禾苗发黄而死。因而纠正了过去认为黑尾叶蝉、白翅叶蝉为害水稻后,都会造成叶片出现白色或褐色斑纹的报道。白翅叶蝉在湖南一年发生三代,在晚稻收割后以成虫迁向麦田、杂草等场所越冬,因其寿命很长,故长期不同世代交错。白翅叶蝉除为害水稻外,在初冬与早春还为害冬小麦。近年在湖南及我国南方部分稻区有回升趋势。