全文获取类型
收费全文 | 4621篇 |
免费 | 300篇 |
国内免费 | 493篇 |
专业分类
林业 | 255篇 |
农学 | 212篇 |
基础科学 | 224篇 |
442篇 | |
综合类 | 2275篇 |
农作物 | 349篇 |
水产渔业 | 357篇 |
畜牧兽医 | 675篇 |
园艺 | 407篇 |
植物保护 | 218篇 |
出版年
2024年 | 38篇 |
2023年 | 116篇 |
2022年 | 210篇 |
2021年 | 198篇 |
2020年 | 189篇 |
2019年 | 196篇 |
2018年 | 156篇 |
2017年 | 243篇 |
2016年 | 170篇 |
2015年 | 219篇 |
2014年 | 254篇 |
2013年 | 314篇 |
2012年 | 394篇 |
2011年 | 404篇 |
2010年 | 351篇 |
2009年 | 316篇 |
2008年 | 409篇 |
2007年 | 314篇 |
2006年 | 233篇 |
2005年 | 180篇 |
2004年 | 127篇 |
2003年 | 84篇 |
2002年 | 90篇 |
2001年 | 81篇 |
2000年 | 76篇 |
1999年 | 23篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1962年 | 2篇 |
1956年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有5414条查询结果,搜索用时 8 毫秒
271.
颗粒日粮中添加柠条对滩羊生长性能血液生化指标瘤胃发酵及羊肉品质的影响 《畜牧与饲料科学》2021,42(3):14-20
[目的]研究颗粒日粮中添加柠条对滩羊生长性能、血液生化指标、瘤胃发酵及羊肉品质的影响。[方法]选择体重相近的健康去势滩羊20只,随机分成两组,每组10只;对照组饲喂含有10%菊花粕的颗粒日粮,试验组饲喂含有6%柠条和4%菊花粕的颗粒日粮;开展周期为50天的育肥试验,测定滩羊的生长性能指标;育肥试验结束后,测定滩羊血液生化指标;屠宰后测定滩羊的屠宰性能指标,并取瘤胃液测定瘤胃发酵指标;取背腰最长肌,测定粗脂肪、粗蛋白、氨基酸含量以及脂肪酸组成。[结果]试验组和对照组滩羊的初始体重和终末体重无显著(P>0.05)差异;试验组平均日增重较对照组降低10.84%,差异不显著(P>0.05);对照组平均日采食量显著(P<0.05)高于试验组;两组试验羊料肉比相同。试验组滩羊血清中的总蛋白含量显著(P<0.05)高于对照组,其他血液生化指标无显著(P>0.05)差异。对照组滩羊屠宰率比试验组高1.59%,差异不显著(P>0.05);两组试验羊肝脏重、肝脏指数、心脏和肺脏重、心脏和肺脏指数差异不显著(P>0.05)。试验组滩羊瘤胃液中丁酸浓度显著(P<0.05)高于对照组,异戊酸浓度显著(P<0.05)低于对照组。对照组和试验组滩羊背腰最长肌中分别检测出18种和20种脂肪酸,粗蛋白含量分别为17.51%和20.92%,粗脂肪含量分别为3.22%和3.89%,总氨基酸含量分别为13.25%和14.09%。[结论]颗粒日粮中加入柠条对滩羊生长性能、血液生化指标和屠宰性能影响不明显,但可改善瘤胃发酵,丰富羊肉中脂肪酸组成及含量,增加肉中粗蛋白、粗脂肪和氨基酸含量。 相似文献
272.
The paper introduced the research progress on the technique of frozen embryo transfer in sheep,illustrated selection of donors and receptors,superovulation,synchronization of estrus,embryo cryopreservation and embryo transplantation. Frozen embryo transfer in sheep is another breakthrough in the high-quality sheep raising,and this technique in China is in its infancy recommendation stage,but it will be comprehensively popularized in the future. 相似文献
273.
AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells. 相似文献
274.
275.
文章主要探讨和阐述了光学系统中的光心如何确定、如何定义、如何计算,并明确指出了确定光心的重要性和实际意义. 相似文献
276.
277.
278.
279.
280.