人员在岗培训在实施兽药GMP现场管理中的作用

论述了对兽药生产企业进行人员在岗培训的必要性,对实施在岗培训的基本程序和应注意的事项进行了总结,旨在为兽药企业进行GMP现场管理提供参考。     

Effect of Ganoderma lucidum spores powder on the expression of IGF-1, NF-κB and apoptosis of nerve cells in the brain from epileptic rat

AIM: To investigate the effect of Ganoderma lucidum spores powder on the expression of insulin-like growth factor-1 (IGF-1), nuclear factor-κB (NF-κB) and apoptosis of nerve cells in rats with epilepsy established by pentetrazole. METHODS: The sub-eclampsia dosage of pentetrazole (PTZ) was used to make epilepsy model. Ganoderma lucidum spores powder group was given from stomach. The enduring time and latent period were recorded. The immune reactivity of IGF-1, NF-κB/P65 and apoptosis of nerve cells were measured with immunohistochemical staining and TUNEL method. RESULTS: In high power sight (×400), there were much more apoptosis cells in hippocampus and brain cortex of model group (18.80±2.13, 16.87±2.00) than those in control group (0.97±0.52, 0.58±0.25). The expressions of IGF-1, NF-κB in model group were higher than those in control group. Compared with model group, the latent period of Ganoderma lucidum spores powder group at the 17th, 21th, 25th days were longer (P<0.05, P<0.05, P<0.01, respectively).There were less apoptosis cells in hippocampus and brain cortex of Ganoderma lucidum spores powder group (12.30±2.46, 10.48±1.33) than those in model group. The expression of NF-κB/P65 in Ganoderma lucidum spores powder group was lower than that in model group, but the immune reactivity of IGF-1 increased more distinctly in Ganoderma lucidum spores powder group than that in model group. CONCLUSION: IGF-1, NF-κB and apoptosis of nerve cells may have play a role in occurrence and development of PTZ-induced epilepsy. Ganoderma lucidum spores powder can suppress expression of NF-κB strongly, and facilitate the immune reactivity of IGF-1, which may be one of the mechanisms by which Ganoderma lucidum spores powder restrains the apoptosis of nerve cells caused by epilepsy to prevent the damages of nerve cells.     

黄河中游多沙粗沙区近40年降水特征分析

本文采用黄河中游多沙粗沙区具有代表性的17个雨量站的近40年旬降水量资料,分析了其降水量变化的特点和分布规律。结果表明:多沙粗沙区降水量年内分布集中,季节差异大;区间降水分配不均匀,且可分为三种明显不同的类型。各区降水量近几年来都呈现出明显的减少趋势。     

卷叶螟绒茧蜂寄生棉大卷叶螟的行为及其与寄主密度的关系

卷叶螟绒茧蜂Apanteles derogatae Watanabe是棉大卷叶螟Sylepta derogata Fabricius幼虫期的一种重要内寄生蜂,对棉大卷叶螟种群有较好的控制作用。在实验室条件下,运用人工接虫观察法研究卷叶螟绒茧蜂对棉大卷叶螟幼虫的寄生行为。结果表明,该蜂搜寻寄主的时间平均为2．38 min,处理寄主的时间仅需0．86 min;寄生寄主的最适龄期为寄主2龄幼虫;对1龄幼虫的致死率达32%,但其寄生率仅为16%,其余16%的个体为穿刺致死。饲喂蜂蜜水能显著增加该蜂的产卵量,延长其产卵历期和增强其穿刺能力。寄生蜂的寄生能力与寄主密度的关系呈抛物线型:在寄生蜂均为16头/m~3雌蜂条件下,当寄主密度从80头/m~3增加到320头/m~3时,寄主的被寄生个体数随之增加,但当寄主密度超过320头/m~3后,寄主的被寄生个体数反而随寄主密度的上升呈下降趋势。     

小麦内生细菌对全蚀病的防治作用及其机制

小麦全蚀病是世界各大小麦产区危害十分严重的一种土传病害,目前对其防治还没有好的抗病品种和特别有效的化学农药。自从成功地用荧光假单胞菌Pseudomonas fluoresens防治小麦全蚀病以来,生物防治逐渐成为防治该病的一种经济而有效的措施[1]。近几年,内生菌由于其独特的优点已成为生物防治的研究热点,但对其防病机制的研究仍不够深入,作者对健康小麦上获得的5株内生细菌防治小麦全蚀病的作用及其机制进行了研究,为其进一步应用提供依据。1材料与方法1.1供试菌及其培养:小麦全蚀病菌9826Gaeu-mannomyces graminisvar.tritici(简称Ggt)与小…     

花椒波瘿蚊研究初报

简述了花椒波瘿蚊的形态特征、危害情况、生活史、主要生活习性、主要天敌及防治措施     

适应不同成熟度大豆的差速脱粒滚筒性能试验与分析

针对南方地区大豆草谷比和未成熟豆荚占比高，造成收获机脱粒清选分离质量差、功耗大等问题，设计了一种前后两段组合且两段转速差可调的脱粒滚筒，研究了脱粒滚筒参数变化对豆荚和籽粒的能量、等效形变量等的影响。以脱粒齿类型、前段滚筒转速、两段滚筒转速差为影响因素，以破碎率、未脱净率和夹带损失率为评价指标，得到了差速与非差速脱粒滚筒的最优参数组合，并通过综合性能试验对比了两种脱粒滚筒的脱粒质量、作业油耗和工作效率。结果表明，差速脱粒滚筒最优参数组合是脱粒齿类型为纹杆齿-杆齿组合式脱粒齿，前段滚筒转速为450 r/min，两段滚筒转速差为150 r/min。此时，相较于传统的杆齿式非差速脱粒滚筒，脱粒质量更高，油耗降低了2.7 L/hm²，最大作业效率增大了10.35%。该研究能够为解决南方地区大豆联合收获机脱粒装置适应性问题提供依据。     

国外抗黄曲霉花生品种资源的引进鉴定研究

为筛选出籽粒和荚果表面均抗黄曲霉感染的花生品种资源，采用福建省花生主产区分离得到的高毒，致病笥强的ＡＦ０４１２２菌株，分别对国外引进的ＩＣＧＶ９１２８７等１１个抗黄曲霉花生品种进行籽粒和荚果表面接菌鉴定。试验结果：ＩＣＧＶ９１２８７，ＩＣＧＶ９４４４９Ａ和ＵＣＧＶ８４３７７等３个品种籽粒与荚果表面均抗黄曲霉素感染。     

鸟氨酸脱羧酶抗酶1基因的结构和功能

鸟氨酸脱羧酶抗酶1(OAZ1)基因可通过特殊的+1移码机制翻译全长的功能蛋白。研究发现,OAZ1能与鸟氨酸脱羧酶(ODC)结合并降解ODC,负调控细胞内多胺的水平;OAZ1还能降解Cyclin D1、Cyclin E1和Smad1周期蛋白,阻滞细胞周期;此外,近年来研究表明,OAZ1还具有抗肿瘤效应和调控动物繁殖的功能。抗酶抑制因子能竞争性结合ODC-OAZ1复合体中的OAZ1,从而阻止ODC降解;天门冬酰胺也能通过抑制OAZ1的翻译来调节ODC的活性。本文就OAZ1基因结构和功能的研究现状作一综述。