Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.     

东欧蛋鸡国际测定总结

1980—1985年间在捷克斯洛伐克国际家禽测定站进行了蛋用配套杂交鸡4次测定(第10—13次)。苏联、捷克和匈牙利4次均提供样本,保加利亚、波兰和民主德国3次提供样本,第11次测定中古巴提供样     

The effect of seed source, light during germination, and cold-moist stratification on seed germination in three species of Echinacea for organic production

Organic production of one of the most popular botanical supplements, Echinacea, continues to expand in the U.S. Echinacea seeds typically show a high degree of dormancy that can be broken by ethephon or gibberelic acid (GA), but these methods are currently disallowed in organic production. In order to determine the efficacy of non-chemical seed treatments, we evaluated the effect of varying seed source and supplying light, with and without cold-moist stratification, on seed germination of the three most important medicinal species of Echinacea, E. angustifolia DC, E. purpurea (L) Moench, and E. pallida (Nutt.) Nutt. Treatments included cold-moist stratification under 24 h light, 24 h dark, and 16/8 h light/dark to break seed dormancy. We found that germination was greater in the E. purpurea and E. pallida seeds from a commercial organic seed source compared to a public germplasm source. When seeds were not cold-moist stratified, 16-24 h light increased germination in E. angustifolia only. Echinacea angustifolia, E. purpurea, and E. pallida seeds that were cold-moist stratified under 16-24 h of light for 4 wk had a significantly greater percentage and rate of germination compared to seeds germinated in the dark. Therefore, cold-moist stratification under light conditions is recommended as a method to break seed dormancy and increase germination rates in organic production of Echinacea.     

制备鸡冻精的方法

人们一般常将用甘油、乙二醇、二甲亚砜等低温保护剂用于家禽精液的低温保存。但是,其中每一种都有自已的不足之处。有些研究者将NN—乙酰替二甲胺(MAA)作为冷冻红血球、血小板、粒性白细胞、牛羊精液的低温保护剂使用,经使用未发现MAA有优于甘油之处。     

种蛋孵化前的深度消毒

经蛋传递的禽传染病对工厂化养禽业特别危险,因为这类传染原即可通过内源途径——即在母鸡体内形成蛋的时候进入蛋内,也可通过外源途径——即在保存、运输和孵化过程进入蛋内。因此,在防制经蛋传递的疾病时采取的综合性措施中,必须包括使用抗菌药物直接作用于种蛋内部病原体的     

PRRS在同源性和异源性感染中的免疫与诊断

通过建立多种不同的与管理活动相接近的模型是更好了解对PRRSV免疫保护机制的关键。更好的在呼吸与生殖两方面了解抗PRRSV的免疫应答将会带来更敏感、更有影响力以及更有区别性的诊断。     

马立克氏病的防治——当前的主要问题和最新进展(续完)

欧洲的情况表5到表7显示了采用MID免疫接种前后的情况. 荷兰的情况值得一提,荷兰自开始采用疫苗以来就一直使用血清I型疫苗病毒,未发生过马立克氏病,而在其他使用HVT的国家则马立克氏病的发生日见增多。德国自1982年以来在用HVT接种过的鸡群中损失日浙增多。损失尤其多见于产蛋早期。有迹象表明,这些迟发型的MD总是发生于未现抗MDV沉淀素的鸡群中。这可能意味着,感染了MDV的鸡发生了